FastQCFastQC Report
Thu 26 May 2016
SRR1512217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987825
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60960.3066668343541308No Hit
GTCCTACAGTGGACATTTCTAAATT58630.29494548061323306No Hit
CTGTAGGACGTGGAATATGGCAAGA50180.25243670846276706No Hit
GGTATCAACGCAGAGTACTTTTTTT40740.2049476186284004No Hit
TATCAACGCAGAGTACTTTTTTTTT37620.18925207198822833No Hit
GTCCTAAAGTGTGTATTTCTCATTT34600.1740595877403695No Hit
CTTTAGGACGTGAAATATGGCGAGG30430.15308188598090877No Hit
GTCCTACAGTGTGCATTTCTCATTT29550.14865493692855256No Hit
CTGTAGGACCTGGAATATGGCGAGA22870.11505036912203036No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21610.10871178297888395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACT400.005278581814.2504914
TAGGACC8850.014.1699794
TCCAACG2800.012.8887618
ATAGGAC2900.011.793513
CCAACGA1701.8189894E-1211.73304119
CCAACGT1552.5465852E-1111.64292619
CGTTTCC1800.011.60742214
GTAGGAC26050.011.5973283
TGTAGGA27150.011.5123672
CCGTTTC1850.011.29370713
CGGTTTC1950.011.20156913
AGGACGT22950.011.1768565
GGCGAGG9100.011.06380919
GGACGTG22500.011.0587076
TAGGACT3450.011.0148734
GATATAC7350.010.9027821
ATCCCGT1752.0008883E-1110.85369310
CTGTAGG27600.010.8534041
TGAGTTA8300.010.75561712
ACTGTTC8600.010.7117128
Produced by FastQC (version 0.11.5)