##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512217_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1987825 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.142128708513074 31.0 31.0 34.0 28.0 34.0 2 31.330744406575025 33.0 31.0 34.0 28.0 34.0 3 31.431308087985613 33.0 31.0 34.0 28.0 34.0 4 35.005457220831815 37.0 35.0 37.0 32.0 37.0 5 34.654895174374005 37.0 35.0 37.0 32.0 37.0 6 34.608786990806536 37.0 35.0 37.0 31.0 37.0 7 34.44858576584961 37.0 35.0 37.0 30.0 37.0 8 34.369007332134366 37.0 35.0 37.0 30.0 37.0 9 35.907801743111186 39.0 35.0 39.0 30.0 39.0 10 35.729576798762466 39.0 35.0 39.0 30.0 39.0 11 35.72403606957353 39.0 35.0 39.0 30.0 39.0 12 35.55475004087382 39.0 35.0 39.0 30.0 39.0 13 35.54679008464025 39.0 35.0 39.0 30.0 39.0 14 36.608291474350104 40.0 36.0 41.0 30.0 41.0 15 36.563828304805504 40.0 36.0 41.0 30.0 41.0 16 36.47336913460692 40.0 36.0 41.0 30.0 41.0 17 36.42468728384038 40.0 36.0 41.0 30.0 41.0 18 36.36622841547923 40.0 36.0 41.0 30.0 41.0 19 36.396232565743965 40.0 36.0 41.0 30.0 41.0 20 36.284684516997224 40.0 36.0 41.0 29.0 41.0 21 36.18226101392225 39.0 36.0 41.0 29.0 41.0 22 36.28199212707356 40.0 36.0 41.0 30.0 41.0 23 36.19330373649592 40.0 36.0 41.0 29.0 41.0 24 36.08626513903387 40.0 36.0 41.0 29.0 41.0 25 35.668158917409734 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1976515220552031 1101 2 0.2331693355426303 1101 3 0.265029174337414 1101 4 0.297678454470244 1101 5 0.32363316520005014 1101 6 0.34332420501000627 1101 7 0.3706428135608064 1101 8 0.2448272525290207 1101 9 0.17611467230342015 1101 10 0.29739766225807074 1101 11 0.4758179609349966 1101 12 0.35652948776373705 1101 13 0.23586488425193863 1101 14 0.1442706927088011 1101 15 0.037270156680889954 1101 16 0.07035405763118519 1101 17 0.13655905809657298 1101 18 0.13808023425808358 1101 19 0.19085367823470278 1101 20 0.23071309049190347 1101 21 0.3177925237146084 1101 22 0.2881751954456888 1101 23 0.05223809158064796 1101 24 0.04860332701751702 1101 25 0.11012579698881808 1102 1 0.26251742482342166 1102 2 0.3356646455662151 1102 3 0.3061381802319225 1102 4 0.3144399143351819 1102 5 0.28615894582284795 1102 6 0.3895170021297645 1102 7 0.31516592354926587 1102 8 0.3581120655393164 1102 9 0.43944044497684587 1102 10 0.38775587738120265 1102 11 0.5318539364661632 1102 12 0.5535581805703558 1102 13 0.5619435496011178 1102 14 0.4765697324436218 1102 15 0.6353092950053068 1102 16 0.5420658792943058 1102 17 0.5743864394925566 1102 18 0.6294190847981298 1102 19 0.5303734842703136 1102 20 0.6407104046602612 1102 21 0.6062622397207846 1102 22 0.569373584881923 1102 23 0.6250447330373206 1102 24 0.7728698304884958 1102 25 0.6806765152222809 1103 1 0.2299622512357864 1103 2 0.3041710475948314 1103 3 0.2515073668433203 1103 4 0.3199054493690099 1103 5 0.2502515172472286 1103 6 0.35122356672272304 1103 7 0.34094861698997647 1103 8 0.25687796978373 1103 9 0.4134927617020523 1103 10 0.39296701783324295 1103 11 0.4453668295050832 1103 12 0.4088138682775977 1103 13 0.5438553051408945 1103 14 0.41618317113353953 1103 15 0.6209664607958345 1103 16 0.5306437881990433 1103 17 0.4671396629285596 1103 18 0.7001921087064247 1103 19 0.4758819956305018 1103 20 0.3281621239812509 1103 21 0.5618241316380619 1103 22 0.4775337564487856 1103 23 0.610120908984733 1103 24 0.6173512589789425 1103 25 0.5936727221483054 1104 1 0.18607631509043543 1104 2 0.23294904414910178 1104 3 0.14446745713297204 1104 4 0.2317610657532967 1104 5 0.30708000460734297 1104 6 0.3512997874179433 1104 7 0.3276210819728007 1104 8 0.21016826475504757 1104 9 0.31013943914494035 1104 10 0.3073102005433981 1104 11 0.3173770380892549 1104 12 0.47893912521062987 1104 13 0.528642485873192 1104 14 0.7292284955620829 1104 15 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-0.1583259133361281 2313 6 -0.1861125421326406 2313 7 -0.20765780788602228 2313 8 -0.16211696145728638 2313 9 -0.19985423955227333 2313 10 -0.05482895842231983 2313 11 -0.2959003316467559 2313 12 -0.07586619371414827 2313 13 -0.11694125633805896 2313 14 -0.16279386980922084 2313 15 -0.21318442199554255 2313 16 -0.2613824477279536 2313 17 -0.4270333134756896 2313 18 -0.2921018145015921 2313 19 -0.08674524756699498 2313 20 -0.13377357388335298 2313 21 -0.20696139637831834 2313 22 -0.08525242160399671 2313 23 -0.128925215006376 2313 24 -0.2396595552631453 2313 25 -0.16688274444821616 2314 1 -0.3230239668222019 2314 2 -0.35854675810074 2314 3 -0.4157574723888686 2314 4 -0.2767054132142732 2314 5 -0.4403565741489288 2314 6 -0.29453472130970226 2314 7 -0.26303025633874455 2314 8 -0.39765512944399717 2314 9 -0.5325352456825527 2314 10 -0.45315118798099263 2314 11 -0.4135838490346728 2314 12 -0.36965067461137835 2314 13 -0.5026311168028883 2314 14 -0.4698616127440616 2314 15 -0.3722630094952706 2314 16 -0.5118958473605772 2314 17 -0.4311246684908525 2314 18 -0.48310525359460144 2314 19 -0.40517916941603005 2314 20 -0.4329637128552548 2314 21 -0.4315247426564923 2314 22 -0.30637335000264443 2314 23 -0.33883779820645543 2314 24 -0.3005690920964881 2314 25 -0.23961416348871012 2315 1 -0.3282655141542641 2315 2 -0.37597934438426606 2315 3 -0.24910257059665142 2315 4 -0.27069704582524423 2315 5 -0.5334604385355561 2315 6 -0.6184683800371147 2315 7 -0.5354582148858995 2315 8 -0.6198683034480652 2315 9 -0.6648768723979401 2315 10 -0.5962349995006093 2315 11 -0.6281518921390727 2315 12 -0.7085867172046889 2315 13 -0.7446256455447369 2315 14 -0.8909771436796987 2315 15 -0.7977840586959744 2315 16 -0.960483411743688 2315 17 -0.9398087232476016 2315 18 -0.9822976179676175 2315 19 -0.9505262535871992 2315 20 -0.9612617193597188 2315 21 -0.9704314453617968 2315 22 -0.7703631725318161 2315 23 -0.7791784485029893 2315 24 -0.8835546386524626 2315 25 -0.9008400029387928 2316 1 -0.6584150727722289 2316 2 -0.6484794534378331 2316 3 -0.6417276412849802 2316 4 -0.6416630953862565 2316 5 -0.6826388427065453 2316 6 -0.8206670571682082 2316 7 -0.8636901991381691 2316 8 -0.7487529192710127 2316 9 -0.9812510986579639 2316 10 -1.0263801959758396 2316 11 -0.955256007054416 2316 12 -1.109896381787351 2316 13 -1.0464611644687736 2316 14 -1.4210440966758426 2316 15 -1.3055115355972546 2316 16 -1.070682926720572 2316 17 -1.2617837835807535 2316 18 -1.214596848383458 2316 19 -1.1907626188455254 2316 20 -1.1018797131591853 2316 21 -0.9900650175126202 2316 22 -1.0147807105463187 2316 23 -0.9874941465971219 2316 24 -1.0187641649434127 2316 25 -1.0579123135107764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24002.0 3 1.0 4 416.0 5 4139.0 6 5311.0 7 4095.0 8 3372.0 9 3106.0 10 2943.0 11 2703.0 12 2573.0 13 2505.0 14 2571.0 15 2525.0 16 2650.0 17 2806.0 18 2969.0 19 3290.0 20 4133.0 21 5291.0 22 7046.0 23 8962.0 24 11030.0 25 13221.0 26 15453.0 27 17895.0 28 21198.0 29 26045.0 30 33729.0 31 44265.0 32 56835.0 33 75181.0 34 99622.0 35 132401.0 36 191493.0 37 329425.0 38 822623.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.458853636416464 19.174672786938256 13.228224321258681 25.138249255386608 2 14.572459849332814 19.706211562889088 41.81530064266221 23.906027945115895 3 19.736194081470952 23.12436959993963 29.446153459182778 27.693282859406636 4 12.406776250424459 16.294945480613233 37.752719681058444 33.54555858790386 5 12.925626405922108 37.713284988903595 36.09119685174095 13.269891753433344 6 32.784475494573215 37.03852200269138 17.254838831386063 12.92216367134934 7 28.957025895136645 31.141574333756743 20.908731905474575 18.992667865632036 8 24.820343843145146 38.99151082212972 19.41795681209362 16.770188522631518 9 25.722485631280417 15.857733955453826 20.09638675436721 38.323393658898546 10 14.690578899047955 29.76061776061776 34.15496836995208 21.3938349703822 11 35.072252336095985 22.358608026360468 23.882534931394865 18.686604706148678 12 23.45639077886635 24.531988479871217 31.674518632173353 20.33710210908908 13 29.52920906015369 21.623935708626263 25.559141272496323 23.287713958723728 14 20.126570497906002 22.698527284846502 27.357237181341414 29.817665035906078 15 22.6352420358935 31.85184812546376 23.05122432809729 22.461685510545447 16 21.49580571730409 28.813904644523536 28.40109164539132 21.289197992781055 17 21.134254775948587 29.32255102939142 28.205853130934567 21.33734106372543 18 20.84640247506697 27.874485933117853 30.74088513827927 20.5382264535359 19 23.041464917686415 27.17045011507552 27.790424207362317 21.997660759875743 20 22.954082980141614 27.73986643693484 28.865217008539485 20.44083357438406 21 22.20592859029341 27.21798951114912 27.565505011759083 23.010576886798383 22 22.212569013871946 28.708211235898535 28.028372718926462 21.050847031303057 23 21.633846037754832 28.146642687359304 28.405820431879064 21.813690843006803 24 22.539509262636297 26.904229497063376 29.45500735728749 21.10125388301284 25 22.119896582066477 27.86161436823043 28.86133112102001 21.15715792868308 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 144.0 1 144.0 2 261.0 3 378.0 4 378.0 5 378.0 6 1067.5 7 1757.0 8 1757.0 9 1757.0 10 2886.0 11 4015.0 12 4015.0 13 4015.0 14 6271.0 15 8527.0 16 8527.0 17 8527.0 18 15028.0 19 21529.0 20 21529.0 21 21529.0 22 35219.5 23 48910.0 24 48910.0 25 48910.0 26 70894.0 27 92878.0 28 92878.0 29 92878.0 30 123390.0 31 153902.0 32 153902.0 33 153902.0 34 174778.5 35 195655.0 36 195655.0 37 195655.0 38 211187.5 39 226720.0 40 226720.0 41 226720.0 42 240375.0 43 254030.0 44 254030.0 45 254030.0 46 259284.0 47 264538.0 48 264538.0 49 264538.0 50 253579.0 51 242620.0 52 242620.0 53 242620.0 54 213805.5 55 184991.0 56 184991.0 57 184991.0 58 157849.0 59 130707.0 60 130707.0 61 130707.0 62 106063.0 63 81419.0 64 81419.0 65 81419.0 66 62397.5 67 43376.0 68 43376.0 69 43376.0 70 31665.0 71 19954.0 72 19954.0 73 19954.0 74 13891.5 75 7829.0 76 7829.0 77 7829.0 78 5344.0 79 2859.0 80 2859.0 81 2859.0 82 1837.5 83 816.0 84 816.0 85 816.0 86 509.0 87 202.0 88 202.0 89 202.0 90 130.0 91 58.0 92 58.0 93 58.0 94 34.0 95 10.0 96 10.0 97 10.0 98 5.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4331367197816709 2 0.0 3 0.0 4 0.0 5 0.03496283626576786 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.008149610755473949 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1987825.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.82320517313237 #Duplication Level Percentage of deduplicated Percentage of total 1 85.82789619221903 63.36090390176492 2 10.14352313596625 14.97654779289703 3 2.13972004450054 4.738829755746819 4 0.7579624478923436 2.2382086921714452 5 0.32760356607940183 1.2092372637064752 6 0.2019959083708243 0.8947191232675563 7 0.12337701326405587 0.6375660594688468 8 0.08953967650266208 0.5288084727673537 9 0.06496169725507814 0.4316112634370889 >10 0.28609464168310805 3.719219480877129 >50 0.019495340826810273 1.0229177477306202 >100 0.013884508412937485 2.0945145147567836 >500 0.002177008014847718 1.144934509971038 >1k 0.0015647245106717661 2.1493449703346856 >5k 2.0409450139196952E-4 0.8526364511022516 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6096 0.3066668343541308 No Hit GTCCTACAGTGGACATTTCTAAATT 5863 0.29494548061323306 No Hit CTGTAGGACGTGGAATATGGCAAGA 5018 0.25243670846276706 No Hit GGTATCAACGCAGAGTACTTTTTTT 4074 0.2049476186284004 No Hit TATCAACGCAGAGTACTTTTTTTTT 3762 0.18925207198822833 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3460 0.1740595877403695 No Hit CTTTAGGACGTGAAATATGGCGAGG 3043 0.15308188598090877 No Hit GTCCTACAGTGTGCATTTCTCATTT 2955 0.14865493692855256 No Hit CTGTAGGACCTGGAATATGGCGAGA 2287 0.11505036912203036 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2161 0.10871178297888395 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 5.030623923132066E-5 0.0 13 0.0 0.0 0.0 5.030623923132066E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTACT 40 0.0052785818 14.250491 4 TAGGACC 885 0.0 14.169979 4 TCCAACG 280 0.0 12.88876 18 ATAGGAC 290 0.0 11.79351 3 CCAACGA 170 1.8189894E-12 11.733041 19 CCAACGT 155 2.5465852E-11 11.642926 19 CGTTTCC 180 0.0 11.607422 14 GTAGGAC 2605 0.0 11.597328 3 TGTAGGA 2715 0.0 11.512367 2 CCGTTTC 185 0.0 11.293707 13 CGGTTTC 195 0.0 11.201569 13 AGGACGT 2295 0.0 11.176856 5 GGCGAGG 910 0.0 11.063809 19 GGACGTG 2250 0.0 11.058707 6 TAGGACT 345 0.0 11.014873 4 GATATAC 735 0.0 10.902782 1 ATCCCGT 175 2.0008883E-11 10.853693 10 CTGTAGG 2760 0.0 10.853404 1 TGAGTTA 830 0.0 10.755617 12 ACTGTTC 860 0.0 10.711712 8 >>END_MODULE