FastQCFastQC Report
Thu 26 May 2016
SRR1512216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786253
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT56210.20174047367557793No Hit
GTATCAACGCAGAGTACTTTTTTTT49520.17772973236816614No Hit
CTGTAGGACGTGGAATATGGCAAGA48740.17493027374039616No Hit
GTCCTAAAGTGTGTATTTCTCATTT44840.16093298060154623No Hit
CTTTAGGACGTGAAATATGGCGAGG42040.15088364193775655No Hit
GGTATCAACGCAGAGTACTTTTTTT29970.10756381419777744No Hit
TATCAACGCAGAGTACTTTTTTTTT29580.10616408488389245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG902.2439053E-713.6176271
TAGGACC8200.012.4101194
CGGTAGG956.0874463E-611.8258341
TCCAACG3200.011.48428718
GGTATCA17450.011.4715821
GGCGAGG13900.011.04999819
AGGACGT30400.010.9412565
TGTAGGA26350.010.7364782
CTAGGAC3700.010.6958083
GTAGGAC27000.010.6439323
CCAACGA2400.010.60088119
GGACGTG30850.010.5984016
GTATTAC2151.8189894E-1210.4507371
GACGTGG15450.010.4287847
TATGTCG1554.1927706E-910.334908516
AGGACCT18350.010.3211015
TAGGACG32900.010.1671334
GGACCTG17350.010.1556616
ACGTGGA15800.010.0183188
GTGTAGG4200.09.9700481
Produced by FastQC (version 0.11.5)