FastQCFastQC Report
Thu 26 May 2016
SRR1512216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786253
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66040.23702083048452527No Hit
GTCCTACAGTGGACATTTCTAAATT62720.22510518606888896No Hit
CTGTAGGACGTGGAATATGGCAAGA54540.19574676097253194No Hit
GTCCTAAAGTGTGTATTTCTCATTT49080.17615055057814205No Hit
CTTTAGGACGTGAAATATGGCGAGG46760.16782395568528774No Hit
TATCAACGCAGAGTACTTTTTTTTT42490.15249871422300848No Hit
GGTATCAACGCAGAGTACTTTTTTT41790.14998637955706104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA600.005873922311.0842754
TAGGACC7850.010.8926474
CTAGTAC1855.456968E-1210.78473
GGACGTG29650.010.5700816
GGCGAGG12500.010.56110719
AGGACGT30850.010.5324995
GACGTGG15950.010.4199697
TGTAGGA29750.010.2832282
TTAGGAC18850.010.2316393
TGCATCG750.002646830410.1341955
CTGTAGG29600.010.0211271
TAGGACG34550.09.9820354
GATATAC7150.09.8712821
GGTATCA19650.09.8532921
GACGTGA15350.09.8373437
TTAAGGT1554.0616214E-89.8072864
GTAGGAC29350.09.7112173
GTCCTAA16250.09.62583351
TAGAAAT7550.09.5637274
TTTAGGA21650.09.3472152
Produced by FastQC (version 0.11.5)