FastQCFastQC Report
Thu 26 May 2016
SRR1512215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735767
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58130.212481545394765No Hit
CTGTAGGACGTGGAATATGGCAAGA50550.1847745074781588No Hit
GTCCTAAAGTGTGTATTTCTCATTT43930.16057654032671642No Hit
GTATCAACGCAGAGTACTTTTTTTT40320.14738097213688153No Hit
CTTTAGGACGTGAAATATGGCGAGG36710.13418540394704664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG4200.013.90590118
CCAACGA2450.012.68833319
TAGGACC7700.012.5997964
GCACCGT1052.2484528E-812.559026
CTACGAC550.003272002611.9881563
CCGGTAC550.003272002611.9881563
GCCGGTT2700.011.51180511
CCTACAC4700.011.2229553
CCGGTTT2800.011.101480512
TAGAACA11950.010.95634
GGCGAGG12700.010.82943619
GGACGTG27400.010.794346
AGGACGT27950.010.7841335
TGTAGGA28550.010.7658452
CCGTGCG700.001602331210.7646789
GTAGGAC28950.010.7369863
GTCCTAC28000.010.7211811
AACAGTG8700.010.6102077
GACGTGA13150.010.601157
GAACAGT7800.010.50610356
Produced by FastQC (version 0.11.5)