##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512215_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2735767 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.439841550833826 31.0 28.0 33.0 16.0 34.0 2 25.479207476367687 31.0 23.0 34.0 2.0 34.0 3 27.180419969975514 31.0 25.0 34.0 16.0 34.0 4 31.643087660608526 35.0 32.0 37.0 19.0 37.0 5 32.054998470264465 35.0 33.0 37.0 20.0 37.0 6 32.25178167585178 35.0 33.0 37.0 22.0 37.0 7 32.2226238564907 35.0 33.0 37.0 22.0 37.0 8 32.21787381747057 35.0 33.0 37.0 21.0 37.0 9 33.63232943448766 38.0 34.0 39.0 22.0 39.0 10 33.474312688178486 38.0 33.0 39.0 19.0 39.0 11 33.48522699484276 38.0 33.0 39.0 19.0 39.0 12 33.34149984264011 38.0 33.0 39.0 18.0 39.0 13 33.342074087449696 38.0 33.0 39.0 18.0 39.0 14 34.30517840152323 39.0 33.0 41.0 17.0 41.0 15 34.23163485779308 39.0 33.0 41.0 17.0 41.0 16 34.168688707773725 39.0 33.0 41.0 17.0 41.0 17 34.125108607567824 39.0 33.0 41.0 17.0 41.0 18 34.05802906460967 39.0 33.0 41.0 17.0 41.0 19 34.059410030167044 39.0 34.0 41.0 16.0 41.0 20 33.94989046947346 39.0 33.0 41.0 11.0 41.0 21 33.86053344455138 39.0 33.0 41.0 10.0 41.0 22 33.73740636538126 39.0 33.0 41.0 10.0 41.0 23 33.60473644137092 39.0 33.0 41.0 2.0 41.0 24 33.46183355526988 39.0 33.0 41.0 2.0 41.0 25 33.31337354387271 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7041770507321807 1101 2 3.615491320387882 1101 3 2.1286272533579584 1101 4 1.1507902356753128 1101 5 0.6951256242760309 1101 6 0.7237606877274985 1101 7 0.5403707815316068 1101 8 0.535541833824908 1101 9 0.42681409342281995 1101 10 0.6095667400940883 1101 11 0.525469653770358 1101 12 0.6646761232862275 1101 13 0.7369061707100144 1101 14 0.6488905660581779 1101 15 0.7605649620539694 1101 16 0.8660988690192681 1101 17 0.5650610846393391 1101 18 0.5867104420594771 1101 19 0.4611962235283613 1101 20 0.6108318776154604 1101 21 0.8213452535672872 1101 22 0.7817822362487448 1101 23 0.7409684702131543 1101 24 0.5820831746208484 1101 25 0.5193543352014842 1102 1 1.599442494747624 1102 2 4.318146794739786 1102 3 2.792973985293976 1102 4 1.9936718545390697 1102 5 1.5290114488761404 1102 6 1.4789547721180156 1102 7 1.4061233325520135 1102 8 1.2406900687945708 1102 9 1.2347746560262394 1102 10 1.4165679121845471 1102 11 1.4292582638089684 1102 12 1.529450668239349 1102 13 1.5330000068931326 1102 14 1.6474771628590617 1102 15 1.5111510072828764 1102 16 1.384321773045741 1102 17 1.54344532347001 1102 18 1.6236933433280925 1102 19 1.6421101093529629 1102 20 1.4922601043115478 1102 21 1.6960860147366148 1102 22 1.6966160751897306 1102 23 1.6732254338094066 1102 24 1.5453595121828982 1102 25 1.5690209788859875 1103 1 1.166918189589211 1103 2 3.8747903136365345 1103 3 2.5036708693318204 1103 4 1.535741101773592 1103 5 1.1141175745930383 1103 6 0.8274500581427517 1103 7 0.7654883756068642 1103 8 0.9127104606182357 1103 9 0.8694776837545461 1103 10 0.9328784299865447 1103 11 1.0119987419057281 1103 12 0.9889389118954952 1103 13 0.899865254193017 1103 14 1.1979338547431837 1103 15 1.3565620299785834 1103 16 1.2546438266069586 1103 17 1.3395215313751123 1103 18 1.1304481349164703 1103 19 1.3183261842536993 1103 20 1.3895274692238857 1103 21 1.2642211759267923 1103 22 1.2542703618843873 1103 23 1.4624033884179894 1103 24 1.5777553880672812 1103 25 1.4911699633797042 1104 1 1.5134433848232796 1104 2 4.186530671655039 1104 3 2.7371576587633655 1104 4 1.9104399262537406 1104 5 1.6576477258168225 1104 6 1.352185110982532 1104 7 1.2632582425907941 1104 8 1.2032372745927802 1104 9 1.4061596773244815 1104 10 1.3944832278435157 1104 11 1.2651950684373574 1104 12 1.317179385894633 1104 13 1.4971636536602517 1104 14 1.7511312413128763 1104 15 1.228154631162333 1104 16 1.5957509802426415 1104 17 1.499641839459585 1104 18 1.463541712582341 1104 19 1.460274010427412 1104 20 1.500049219957802 1104 21 1.4508708071582603 1104 22 1.4978590022670986 1104 23 1.5813677187607738 1104 24 1.7863961324384832 1104 25 1.5490664363212971 1105 1 1.0360365345917621 1105 2 3.7953911046804336 1105 3 2.4711664947218743 1105 4 1.7141275297019511 1105 5 1.058288970103611 1105 6 0.9201428831672729 1105 7 0.6523149325112456 1105 8 0.821319483833733 1105 9 0.8386667115038264 1105 10 0.8862405818670922 1105 11 0.8830368709106651 1105 12 0.8452133282783905 1105 13 0.9627901234776104 1105 14 1.0887968332550741 1105 15 1.1177509273123576 1105 16 0.885433206478119 1105 17 0.9318317222092958 1105 18 1.046151731216483 1105 19 1.145557466013642 1105 20 1.1639146316622444 1105 21 1.082882097749156 1105 22 1.3725198596770127 1105 23 0.9751773417335414 1105 24 0.9986866778551615 1105 25 1.064169277160346 1106 1 -0.05516530542766063 1106 2 2.6515783084495617 1106 3 1.175807965511833 1106 4 0.37921680257963786 1106 5 -0.19095255513036946 1106 6 -0.13476971767790502 1106 7 -0.32494747000213664 1106 8 -0.3414634306420119 1106 9 -0.23754505099767442 1106 10 -0.10462089462652102 1106 11 -0.254308782641516 1106 12 -0.034881371324942734 1106 13 -0.2791178095585991 1106 14 -0.16787904097216 1106 15 -0.4816419688032667 1106 16 -0.15050544618551243 1106 17 -0.06614901733379952 1106 18 -0.3035337131386626 1106 19 -0.3875928454485944 1106 20 -0.2479057902671471 1106 21 -0.38343007390742656 1106 22 -0.47064791629643565 1106 23 -0.4716180618710766 1106 24 -0.34321942859586585 1106 25 -0.7457680532913002 1107 1 0.9887075050995904 1107 2 3.7101728225422086 1107 3 2.2675924413720594 1107 4 1.6156342916878437 1107 5 1.050648538494599 1107 6 1.1103389779742656 1107 7 0.8390573757345052 1107 8 0.8344034671974114 1107 9 0.7416838544882367 1107 10 0.8532567616373683 1107 11 0.9138338331671463 1107 12 0.869702073149135 1107 13 0.8462934112113842 1107 14 1.1124579377777266 1107 15 0.9512279076082208 1107 16 1.0126580640838938 1107 17 0.9829040634877586 1107 18 0.9176152599208223 1107 19 1.0989202247034484 1107 20 0.9877303793780925 1107 21 1.0403229268927845 1107 22 0.958816607576594 1107 23 1.024883227361613 1107 24 1.1814760457528521 1107 25 1.4149881483624753 1108 1 1.0171025141284566 1108 2 3.4486130276449423 1108 3 2.138398287110384 1108 4 1.32429954581551 1108 5 0.9706534280526711 1108 6 0.8376731598658296 1108 7 0.8100679878864767 1108 8 0.7173214468009661 1108 9 0.7432340254934147 1108 10 0.6353292874951109 1108 11 0.857324770668761 1108 12 0.7516880615901158 1108 13 0.9145654827800271 1108 14 0.9186922536793958 1108 15 0.9882328075145708 1108 16 0.9194106990124169 1108 17 0.9032500579307339 1108 18 0.9990993572152647 1108 19 1.0467862818569458 1108 20 1.0072936564685762 1108 21 1.0205369032676828 1108 22 1.1297959162086073 1108 23 1.0310861103899285 1108 24 1.209479760782159 1108 25 0.9807220477462337 1109 1 0.6436408880413715 1109 2 3.198703653179983 1109 3 1.8723742148234415 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-2.0701393633931815 2316 1 -1.7992691564803849 2316 2 -0.6198619221318538 2316 3 -0.9181814251152787 2316 4 -1.5656111281364495 2316 5 -1.8064118292989022 2316 6 -1.8389264696112768 2316 7 -1.8424835462494755 2316 8 -1.9174206281909463 2316 9 -1.8737098466385973 2316 10 -1.8644761699337273 2316 11 -2.6549093235115073 2316 12 -2.1989160716749403 2316 13 -2.109725047435557 2316 14 -2.4489608174490627 2316 15 -2.57513752359646 2316 16 -2.3202513066322936 2316 17 -2.3832741947953586 2316 18 -2.9507873472229633 2316 19 -2.853556949856209 2316 20 -2.7971201236868524 2316 21 -2.7450707311265106 2316 22 -2.675368530669271 2316 23 -2.691746582361727 2316 24 -2.577908464847294 2316 25 -2.5776074857062774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 151619.0 3 7.0 4 1326.0 5 6428.0 6 7417.0 7 6804.0 8 6433.0 9 6108.0 10 5954.0 11 5616.0 12 5599.0 13 5580.0 14 5778.0 15 5878.0 16 5915.0 17 6667.0 18 7620.0 19 9326.0 20 12082.0 21 15499.0 22 19385.0 23 22656.0 24 26189.0 25 29446.0 26 33243.0 27 36737.0 28 42337.0 29 50283.0 30 60671.0 31 76001.0 32 94981.0 33 121922.0 34 160127.0 35 215749.0 36 299662.0 37 371362.0 38 797360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.456442014250484 18.423060150955838 12.7466630016372 25.37383483315648 2 15.994091827160101 20.283043623467297 40.54863308894861 23.17423146042399 3 19.495848878943274 23.36130964369407 29.266088815312123 27.87675266205053 4 12.41615239894333 16.127469919770217 37.873327662772446 33.583050018514 5 13.34130428505059 37.512697536010926 35.81368588772362 13.33231229121486 6 32.681779070285984 36.63924473072624 17.672271678206396 13.006704520781382 7 28.835374221278602 31.131747852553065 21.36425481452887 18.66862311163946 8 24.989993665396213 38.02345009644462 19.98061238402247 17.005943854136703 9 26.17660787632865 15.75338835507556 19.891204185151732 38.178799583444054 10 15.017847580021677 29.25683517535956 34.03965763351872 21.685659611100043 11 35.015129549739626 22.21130486974843 24.22570261570505 18.547862964806892 12 23.5557878692066 24.120492805485853 31.687663231308242 20.636056093999304 13 29.34003517112386 21.54978841399871 25.603203781608595 23.50697263326884 14 20.72671473124144 22.169821665903928 27.171934421243936 29.931529181610696 15 22.9867528923333 31.23464096174857 22.987666712845066 22.790939433073063 16 22.19743128709426 28.143295828921104 27.83248719646081 21.826785687523827 17 21.737633358396383 28.65510842114844 27.788733470357673 21.818524750097502 18 21.5503835161533 27.608264442265668 29.872439113017908 20.968912928563125 19 23.647511275994006 26.778178287730398 27.037528891364815 22.536781544910788 20 23.49235150508066 27.571829033685987 27.901133393304328 21.03468606792903 21 22.955199132027964 26.858331639598592 26.812710467865585 23.373758760507858 22 23.05892467521264 28.164686865910276 27.200404428388502 21.57598403048858 23 22.581944591675228 27.7472593751485 27.432676948046016 22.238119085130258 24 23.315948339993295 26.685751531437933 28.279134242685345 21.719165885883427 25 22.929176100909416 27.28361070067941 27.894116176256635 21.89309702215454 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 270.0 1 270.0 2 347.5 3 425.0 4 425.0 5 425.0 6 1089.5 7 1754.0 8 1754.0 9 1754.0 10 2800.0 11 3846.0 12 3846.0 13 3846.0 14 6762.0 15 9678.0 16 9678.0 17 9678.0 18 18444.5 19 27211.0 20 27211.0 21 27211.0 22 44859.0 23 62507.0 24 62507.0 25 62507.0 26 92178.5 27 121850.0 28 121850.0 29 121850.0 30 163312.5 31 204775.0 32 204775.0 33 204775.0 34 232410.5 35 260046.0 36 260046.0 37 260046.0 38 281720.5 39 303395.0 40 303395.0 41 303395.0 42 322965.5 43 342536.0 44 342536.0 45 342536.0 46 351097.5 47 359659.0 48 359659.0 49 359659.0 50 348009.5 51 336360.0 52 336360.0 53 336360.0 54 301324.0 55 266288.0 56 266288.0 57 266288.0 58 229273.5 59 192259.0 60 192259.0 61 192259.0 62 157778.0 63 123297.0 64 123297.0 65 123297.0 66 95481.0 67 67665.0 68 67665.0 69 67665.0 70 49765.5 71 31866.0 72 31866.0 73 31866.0 74 22529.5 75 13193.0 76 13193.0 77 13193.0 78 8997.5 79 4802.0 80 4802.0 81 4802.0 82 3152.0 83 1502.0 84 1502.0 85 1502.0 86 961.0 87 420.0 88 420.0 89 420.0 90 272.5 91 125.0 92 125.0 93 125.0 94 77.5 95 30.0 96 30.0 97 30.0 98 19.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 8.063808065526048 3 0.0 4 0.0 5 0.0 6 3.655282047045673E-5 7 0.007054694350798149 8 0.0 9 0.0 10 0.0037283876879865866 11 0.002229722048697861 12 7.310564094091346E-5 13 0.0 14 1.096584614113702E-4 15 0.0 16 0.0 17 0.0 18 0.010563765115961994 19 0.0032166482014001924 20 0.0 21 0.007127799991739063 22 0.0019738523054046637 23 0.002814567176225168 24 0.009723050245141491 25 0.006031215377625361 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2735767.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.45260425393074 #Duplication Level Percentage of deduplicated Percentage of total 1 86.51542402573638 65.27814050874898 2 9.466607383412457 14.28560361055918 3 2.109772733980072 4.775635414881956 4 0.7398977139961145 2.233088376101475 5 0.3573456706136301 1.348133073333286 6 0.21188300473075494 0.9592274702450033 7 0.1282684039378637 0.6774729584424873 8 0.08727574151308447 0.5268145588284093 9 0.06662194488433931 0.4524119317786716 >10 0.28510160113257965 3.6831868558798435 >50 0.017630351357758502 0.937282081617309 >100 0.011128184294358906 1.7591269238892902 >500 0.00157070472808841 0.8248928207812475 >1k 0.0013743666369614026 1.8564810499112305 >5k 9.816904549724305E-5 0.40250236500160097 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5813 0.212481545394765 No Hit CTGTAGGACGTGGAATATGGCAAGA 5055 0.1847745074781588 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4393 0.16057654032671642 No Hit GTATCAACGCAGAGTACTTTTTTTT 4032 0.14738097213688153 No Hit CTTTAGGACGTGAAATATGGCGAGG 3671 0.13418540394704664 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.655282047045673E-5 0.0 5 0.0 0.0 0.0 3.655282047045673E-5 0.0 6 0.0 0.0 0.0 3.655282047045673E-5 0.0 7 0.0 0.0 0.0 3.655282047045673E-5 0.0 8 0.0 0.0 0.0 3.655282047045673E-5 0.0 9 0.0 0.0 0.0 3.655282047045673E-5 0.0 10 0.0 0.0 0.0 3.655282047045673E-5 3.655282047045673E-5 11 0.0 0.0 0.0 3.655282047045673E-5 3.655282047045673E-5 12 0.0 0.0 0.0 3.655282047045673E-5 3.655282047045673E-5 13 0.0 0.0 0.0 3.655282047045673E-5 3.655282047045673E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 420 0.0 13.905901 18 CCAACGA 245 0.0 12.688333 19 TAGGACC 770 0.0 12.599796 4 GCACCGT 105 2.2484528E-8 12.55902 6 CTACGAC 55 0.0032720026 11.988156 3 CCGGTAC 55 0.0032720026 11.988156 3 GCCGGTT 270 0.0 11.511805 11 CCTACAC 470 0.0 11.222955 3 CCGGTTT 280 0.0 11.1014805 12 TAGAACA 1195 0.0 10.9563 4 GGCGAGG 1270 0.0 10.829436 19 GGACGTG 2740 0.0 10.79434 6 AGGACGT 2795 0.0 10.784133 5 TGTAGGA 2855 0.0 10.765845 2 CCGTGCG 70 0.0016023312 10.764678 9 GTAGGAC 2895 0.0 10.736986 3 GTCCTAC 2800 0.0 10.721181 1 AACAGTG 870 0.0 10.610207 7 GACGTGA 1315 0.0 10.60115 7 GAACAGT 780 0.0 10.5061035 6 >>END_MODULE