FastQCFastQC Report
Thu 26 May 2016
SRR1512215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735767
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT69820.2552117925247289No Hit
CTGTAGGACGTGGAATATGGCAAGA60170.21993832077073813No Hit
GTATCAACGCAGAGTACTTTTTTTT52890.19332786746824565No Hit
GTCCTAAAGTGTGTATTTCTCATTT47630.1741010839007854No Hit
CTTTAGGACGTGAAATATGGCGAGG45440.16609601621775538No Hit
GGTATCAACGCAGAGTACTTTTTTT33120.1210629413981527No Hit
TATCAACGCAGAGTACTTTTTTTTT32290.11802905729910479No Hit
GTCCTACAGTGTGCATTTCTCATTT31540.11528759576382053No Hit
TTGTAGAACAGTGTATATCAATGAG27400.10015472808905145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC1006.6393113E-1014.2454768
TAGGACC9750.012.7651834
TCCAACG4050.012.42815218
CGTATAC550.003067525812.0919323
AGGACCG2000.011.4009665
TGTAGGA32050.011.1460292
GTCCTAG5000.011.0654441
CCAACGA2500.011.01650119
AGGACGT31000.010.9718975
GGCGAGG13550.010.86370919
GGACCGT1408.401912E-910.8536976
GGTATCA14900.010.8196021
CTGTAGG31600.010.807041
GCCGGTT2750.010.70569211
GGACGTG31200.010.592796
GTAGGAC30500.010.528763
TAGGACG33300.010.4993884
TTAGGAC17650.010.496643
GTCCTAC29500.010.4445891
TGTAGAA12850.010.351072
Produced by FastQC (version 0.11.5)