FastQCFastQC Report
Thu 26 May 2016
SRR1512214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988427
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT48570.16252697489348075No Hit
GTATCAACGCAGAGTACTTTTTTTT43760.14643155077905534No Hit
CTGTAGGACGTGGAATATGGCAAGA41800.13987291642057845No Hit
GTCCTAAAGTGTGTATTTCTCATTT37790.1264544859218579No Hit
CTTTAGGACGTGAAATATGGCGAGG33340.11156370893449966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8100.013.1427874
CGCTTAA550.001760105313.020611
GTCCTAA12300.012.1434971
GTAGGAC26100.010.7564723
GTCCTAC25400.010.75408651
AGGACGT27250.010.7519675
GGACGTG27000.010.7119536
TGTAGGA25250.010.6964232
TTAGGAC15500.010.6365413
GTATAAA3950.010.61897851
TCCAACG3150.010.46823218
AGCGACC901.0371151E-410.4668318
GTCTAGG2550.010.4310781
GGCGAGG12750.010.41897119
TAGGACG28900.010.3988884
GTATAAG3350.010.3831741
GACGTGA13800.010.1718817
CTTACAC3550.010.0843833
CTGTAGG26800.010.0014391
GTCCTAG3700.09.95397951
Produced by FastQC (version 0.11.5)