FastQCFastQC Report
Thu 26 May 2016
SRR1512214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988427
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61850.2069650689141813No Hit
GTCCTACAGTGGACATTTCTAAATT54270.1816005544053778No Hit
CTGTAGGACGTGGAATATGGCAAGA51030.17075873026177316No Hit
GTCCTAAAGTGTGTATTTCTCATTT41570.13910328075606332No Hit
CTTTAGGACGTGAAATATGGCGAGG39910.13354851900347575No Hit
TATCAACGCAGAGTACTTTTTTTTT39670.1327454209187643No Hit
GGTATCAACGCAGAGTACTTTTTTT38320.12822799419226236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9050.011.757584
TCCAACG1900.011.49661718
GGTATCA18900.011.3024671
AGGACGT25100.011.0145755
TTTCGCA700.001495766210.8539516
GGACGTG25200.010.70326
ATAGGAC3300.010.6521253
GTCCTAC25650.010.5960641
GACGTGG13600.010.4050277
GTAGGAC26550.010.3772423
GTCCTAA14750.010.2799781
TAGGACA8600.010.2738444
TGTAGGA27250.010.2501292
ACACCGT1303.8605685E-710.227766
TTAGGAC15400.010.1791553
TAGGACG27350.010.1779144
GCACCGT1502.458728E-810.1303536
GGCGAGG12200.09.88709719
GTCCTAG3800.09.7874161
TCCTACA27900.09.7389092
Produced by FastQC (version 0.11.5)