FastQCFastQC Report
Thu 26 May 2016
SRR1512213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2705492
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50930.18824672185317864No Hit
GTATCAACGCAGAGTACTTTTTTTT48950.18092827478329265No Hit
CTGTAGGACGTGGAATATGGCAAGA46460.1717247731651027No Hit
GTCCTAAAGTGTGTATTTCTCATTT41540.15353954105205264No Hit
CTTTAGGACGTGAAATATGGCGAGG37580.13890264691228066No Hit
GGTATCAACGCAGAGTACTTTTTTT30470.11262276879769004No Hit
TATCAACGCAGAGTACTTTTTTTTT30010.11092252351882763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8400.012.5620424
ACCGTCC752.2409816E-411.3058398
ATAGGAC4250.011.0841563
TCCAACG2750.010.96465618
GGTATCA16500.010.961711
GTATTAG2450.010.8441681
GGCGAGG12500.010.77962719
AACCGTG1159.815612E-710.6506247
CGTTAGG1251.0095428E-610.6272841
GTCCTAC24650.010.4465431
AGGACCT16700.010.4370265
TGTAGGA25700.010.3775852
AGGACGT28550.010.2960355
GGACGTG27900.010.1984076
GGACCTG16850.010.0647316
GACGTGG14800.09.9946487
GTCTAGA3800.09.9496341
TTAGGAC18000.09.9449513
TCCTACA27600.09.9223532
TAGGACT4600.09.8311644
Produced by FastQC (version 0.11.5)