##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512213_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2705492 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.724488189209207 31.0 28.0 33.0 20.0 34.0 2 25.818694344688506 31.0 25.0 34.0 2.0 34.0 3 27.48070665150738 31.0 26.0 34.0 16.0 34.0 4 31.971484668962244 35.0 32.0 37.0 19.0 37.0 5 32.36875067455383 35.0 33.0 37.0 25.0 37.0 6 32.565888570359846 35.0 33.0 37.0 25.0 37.0 7 32.54106572852553 35.0 33.0 37.0 25.0 37.0 8 32.54128343384493 36.0 33.0 37.0 25.0 37.0 9 33.97825423250189 38.0 34.0 39.0 25.0 39.0 10 33.829547453845734 38.0 34.0 39.0 24.0 39.0 11 33.83681415432017 38.0 34.0 39.0 24.0 39.0 12 33.695771416067764 38.0 34.0 39.0 23.0 39.0 13 33.693499740527784 38.0 34.0 39.0 23.0 39.0 14 34.67074306632583 39.0 34.0 41.0 22.0 41.0 15 34.591254751446314 39.0 34.0 41.0 22.0 41.0 16 34.53409028745973 39.0 34.0 41.0 21.0 41.0 17 34.492564753471825 39.0 34.0 41.0 19.0 41.0 18 34.43336997485115 39.0 34.0 41.0 19.0 41.0 19 34.44132453542646 39.0 34.0 41.0 18.0 41.0 20 34.3398173049486 39.0 34.0 41.0 18.0 41.0 21 34.2436776748924 39.0 34.0 41.0 18.0 41.0 22 34.12042726424621 39.0 34.0 41.0 18.0 41.0 23 33.98791088644875 39.0 34.0 41.0 15.0 41.0 24 33.85085115757134 39.0 34.0 41.0 8.0 41.0 25 33.69744985385283 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6830047429216783 1101 2 3.563106399033618 1101 3 2.1611420261341756 1101 4 1.06342950706766 1101 5 0.7682477916956003 1101 6 0.808366031616174 1101 7 0.6246256562678525 1101 8 0.443937887249092 1101 9 0.5874960863015488 1101 10 0.43805938506994835 1101 11 0.4820850279383322 1101 12 0.5819417371707303 1101 13 0.49869421475246156 1101 14 0.5726982772352969 1101 15 0.7010419508819865 1101 16 0.8573679575777646 1101 17 0.7218725306311597 1101 18 0.6328784717603781 1101 19 0.4537313172939861 1101 20 0.5530466261708966 1101 21 0.5734940021649777 1101 22 0.5086610606454514 1101 23 0.5925952954319698 1101 24 0.43881338011048854 1101 25 0.4812689208248315 1102 1 1.4204127383034049 1102 2 4.233676947934374 1102 3 2.6411030594723215 1102 4 1.9141222477228723 1102 5 1.393859953142659 1102 6 1.3549720834755092 1102 7 1.2348868772232535 1102 8 1.0947773542236447 1102 9 1.1222918721292316 1102 10 1.2434954749338019 1102 11 1.3291456908526555 1102 12 1.3786488137089492 1102 13 1.2515373378582524 1102 14 1.4887708225761358 1102 15 1.5872208126706013 1102 16 1.4496684338369619 1102 17 1.4569304995540477 1102 18 1.51817697563299 1102 19 1.5163594021547127 1102 20 1.4433001500079072 1102 21 1.6265608227740103 1102 22 1.4620165231941584 1102 23 1.4764241307617425 1102 24 1.5441508506449608 1102 25 1.5247047220467493 1103 1 1.0081731969408487 1103 2 3.6926451671839438 1103 3 2.232748428968634 1103 4 1.3708778636523888 1103 5 1.0234848927047437 1103 6 0.8134560319325175 1103 7 0.7182512929133225 1103 8 0.8200031809759594 1103 9 0.7751111570047868 1103 10 0.9001390408859606 1103 11 0.8707899091505737 1103 12 0.8153620655374354 1103 13 0.8206153814349477 1103 14 0.9511772877063365 1103 15 1.1750288541751388 1103 16 0.9032459747236956 1103 17 1.1212287673841885 1103 18 0.9596254902033223 1103 19 1.1242052029666922 1103 20 1.0079737085156495 1103 21 1.2071784790875668 1103 22 1.2701301694999643 1103 23 1.2573717972825875 1103 24 1.4189864211405094 1103 25 1.401072153876619 1104 1 1.2556893524814576 1104 2 3.777909285851294 1104 3 2.4490657859773712 1104 4 1.481277512876197 1104 5 1.1726484435978648 1104 6 0.9159694805904834 1104 7 0.8478009064182075 1104 8 0.7765650970014448 1104 9 1.0371160696612165 1104 10 0.9834448082610905 1104 11 0.9171202562131953 1104 12 0.9329074375138191 1104 13 0.9889875084481474 1104 14 1.289378187614318 1104 15 0.970994927912713 1104 16 1.135036409808471 1104 17 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-1.5649258864697373 2315 25 -1.4936501453166784 2316 1 -1.6739501908408592 2316 2 -0.5160845134338814 2316 3 -0.9727824920450132 2316 4 -1.6694545051480993 2316 5 -1.8093310329505137 2316 6 -1.6601438317819515 2316 7 -1.8956685948980123 2316 8 -1.9295578413630174 2316 9 -1.945683002469032 2316 10 -1.9442117758396336 2316 11 -2.2876706569281566 2316 12 -1.9042025614549978 2316 13 -1.8170779366238712 2316 14 -2.137141906826443 2316 15 -2.4183417930823623 2316 16 -2.125092526925563 2316 17 -2.187711266983463 2316 18 -2.6198442800864292 2316 19 -2.5078700404708627 2316 20 -2.6118284305745973 2316 21 -2.5174963614188606 2316 22 -2.3421375031777494 2316 23 -2.1714012379343792 2316 24 -2.141300420392998 2316 25 -2.0986720638642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 131640.0 3 4.0 4 1157.0 5 5775.0 6 6858.0 7 6227.0 8 5617.0 9 5751.0 10 5331.0 11 5123.0 12 5177.0 13 5147.0 14 5160.0 15 5420.0 16 5574.0 17 6210.0 18 7048.0 19 8479.0 20 10878.0 21 14400.0 22 17834.0 23 21283.0 24 24825.0 25 27748.0 26 31522.0 27 35072.0 28 40486.0 29 48470.0 30 59523.0 31 74941.0 32 95036.0 33 122407.0 34 161372.0 35 219132.0 36 303543.0 37 379017.0 38 796305.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92612951729297 18.805599868711496 12.978267908387828 25.290002705607705 2 16.053118332848268 20.519253110635933 40.10132667550209 23.326301881013716 3 18.920699081719704 23.95989343158287 29.550854336290776 27.568553150406654 4 12.573424722749134 16.547858947651665 37.75464869236353 33.12406763723567 5 13.412791462698836 37.48741448875103 35.66172067779169 13.438073370758444 6 32.2446819205999 36.72978774993643 17.859550661470315 13.165979667993351 7 28.370331626193718 31.35188084153173 21.933090723596784 18.344696808677767 8 25.10449116094226 37.388208872914795 20.228039853749337 17.279260112393604 9 26.262506043263112 15.637562410090291 20.29346233513165 37.80646921151495 10 15.36690143291937 28.951912961773292 33.927395203494065 21.753790401813273 11 34.51237087528142 22.360278063314958 24.463827706310315 18.6635233550933 12 23.569895970593073 24.252952797742363 31.50943361356651 20.66771761809805 13 29.089866094595735 21.75622770276164 25.754613208983802 23.399292993658825 14 20.977762204470622 22.12228939442998 27.290385010593297 29.6095633905061 15 22.941150814713183 30.939991690975244 23.22690290712373 22.891954587187836 16 22.3231116558467 27.946229373437436 27.64580342503323 22.084855545682633 17 21.786589490258695 28.522818417366167 27.799400478286746 21.89119161408839 18 21.701055812959712 27.418828972495334 29.724635463220284 21.155479751324663 19 23.636018874661513 26.78376714055383 27.271601588807087 22.308612395977566 20 23.608718857790006 27.337800296581914 27.935584359517602 21.117896486110475 21 22.99562454557115 26.732226402875227 27.006907503329554 23.265241548224072 22 23.2646127369663 27.892755654516364 27.19579160272162 21.646840005795713 23 22.536993290882794 27.607069208770678 27.602559747588458 22.253377752758073 24 23.3269457111257 26.58478089864381 28.18989277074942 21.898380619481063 25 22.93969159183547 27.22792314820736 27.857203848282136 21.975181411675035 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 420.0 1 420.0 2 530.0 3 640.0 4 640.0 5 640.0 6 1525.5 7 2411.0 8 2411.0 9 2411.0 10 3580.5 11 4750.0 12 4750.0 13 4750.0 14 7907.5 15 11065.0 16 11065.0 17 11065.0 18 20050.5 19 29036.0 20 29036.0 21 29036.0 22 46912.5 23 64789.0 24 64789.0 25 64789.0 26 94066.0 27 123343.0 28 123343.0 29 123343.0 30 162011.5 31 200680.0 32 200680.0 33 200680.0 34 228023.0 35 255366.0 36 255366.0 37 255366.0 38 275838.5 39 296311.0 40 296311.0 41 296311.0 42 316720.0 43 337129.0 44 337129.0 45 337129.0 46 344738.5 47 352348.0 48 352348.0 49 352348.0 50 340270.5 51 328193.0 52 328193.0 53 328193.0 54 295796.0 55 263399.0 56 263399.0 57 263399.0 58 227459.0 59 191519.0 60 191519.0 61 191519.0 62 157198.5 63 122878.0 64 122878.0 65 122878.0 66 95634.0 67 68390.0 68 68390.0 69 68390.0 70 50341.0 71 32292.0 72 32292.0 73 32292.0 74 22813.0 75 13334.0 76 13334.0 77 13334.0 78 9192.0 79 5050.0 80 5050.0 81 5050.0 82 3284.5 83 1519.0 84 1519.0 85 1519.0 86 1004.5 87 490.0 88 490.0 89 490.0 90 304.5 91 119.0 92 119.0 93 119.0 94 67.5 95 16.0 96 16.0 97 16.0 98 10.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.7984337044796295 3 0.0 4 0.0 5 0.0 6 1.4784741555325242E-4 7 0.006911866677114551 8 0.0 9 0.0 10 0.0039179565121611895 11 0.0024025205027403515 12 2.5873297721819174E-4 13 0.0 14 2.9569483110650484E-4 15 0.0 16 0.0 17 7.392370777662621E-5 18 0.010275395380951043 19 0.002439482356628665 20 0.0 21 0.006394400722678167 22 0.0016263215710857766 23 0.0025503679182936044 24 0.009610082010961406 25 0.006690095553784673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2705492.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.44448946920826 #Duplication Level Percentage of deduplicated Percentage of total 1 84.92279859452074 62.37111587070972 2 10.276593512354614 15.095183279949245 3 2.513236581187933 5.537501328620583 4 0.8908007928838638 2.6169763780848525 5 0.44277754228171007 1.6259785270655487 6 0.2532421440971618 1.115954399118223 7 0.15171972483304444 0.7800084412940194 8 0.10330034269605773 0.6069472745044973 9 0.07949794301515198 0.5254817254740043 >10 0.33287858840662765 4.1108610837636625 >50 0.017414605540529943 0.8827324797034001 >100 0.012682872711602733 1.810565042390835 >500 0.0016302695846039847 0.8233605165453862 >1k 0.0013755399618278353 1.9067690033623261 >5k 5.094592451214205E-5 0.19056464941374268 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5093 0.18824672185317864 No Hit GTATCAACGCAGAGTACTTTTTTTT 4895 0.18092827478329265 No Hit CTGTAGGACGTGGAATATGGCAAGA 4646 0.1717247731651027 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4154 0.15353954105205264 No Hit CTTTAGGACGTGAAATATGGCGAGG 3758 0.13890264691228066 No Hit GGTATCAACGCAGAGTACTTTTTTT 3047 0.11262276879769004 No Hit TATCAACGCAGAGTACTTTTTTTTT 3001 0.11092252351882763 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.6961853888313105E-5 0.0 10 0.0 0.0 0.0 3.6961853888313105E-5 0.0 11 0.0 0.0 0.0 1.4784741555325242E-4 0.0 12 0.0 0.0 0.0 1.4784741555325242E-4 0.0 13 0.0 0.0 0.0 1.4784741555325242E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 840 0.0 12.562042 4 ACCGTCC 75 2.2409816E-4 11.305839 8 ATAGGAC 425 0.0 11.084156 3 TCCAACG 275 0.0 10.964656 18 GGTATCA 1650 0.0 10.96171 1 GTATTAG 245 0.0 10.844168 1 GGCGAGG 1250 0.0 10.779627 19 AACCGTG 115 9.815612E-7 10.650624 7 CGTTAGG 125 1.0095428E-6 10.627284 1 GTCCTAC 2465 0.0 10.446543 1 AGGACCT 1670 0.0 10.437026 5 TGTAGGA 2570 0.0 10.377585 2 AGGACGT 2855 0.0 10.296035 5 GGACGTG 2790 0.0 10.198407 6 GGACCTG 1685 0.0 10.064731 6 GACGTGG 1480 0.0 9.994648 7 GTCTAGA 380 0.0 9.949634 1 TTAGGAC 1800 0.0 9.944951 3 TCCTACA 2760 0.0 9.922353 2 TAGGACT 460 0.0 9.831164 4 >>END_MODULE