FastQCFastQC Report
Thu 26 May 2016
SRR1512213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2705492
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66280.24498316757173927No Hit
GTCCTACAGTGGACATTTCTAAATT55890.20657980138178195No Hit
CTGTAGGACGTGGAATATGGCAAGA52540.19419758032919707No Hit
GTCCTAAAGTGTGTATTTCTCATTT44800.16558910541964272No Hit
GGTATCAACGCAGAGTACTTTTTTT43770.16178203446914646No Hit
CTTTAGGACGTGAAATATGGCGAGG43050.15912078098918792No Hit
TATCAACGCAGAGTACTTTTTTTTT42760.15804888722642682No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA350.00217513616.28080412
TTACGCT400.005279347814.2504444
TAGGACC8400.011.6491724
CTACACT4050.010.7904594
TATACCG803.771533E-410.6878345
TGTAGGA27600.010.6706232
GTCCTAA14300.010.6056171
GGTATCA17850.010.5803941
TAGGACT4700.010.5109654
GTAGGAC27450.010.4866663
GTCCTAC26300.010.4813431
GCCGGTC1002.4081777E-510.44684912
AGGCGTG2100.010.4016257
AAGGCGT2200.010.3605116
TCCAACG3550.010.16594818
CTGTAGG27350.010.1138271
TTAAGAC2750.010.0184933
GGCGAGG10650.09.98833719
GCGTGCG1054.1208965E-59.949389
GGACGTG25600.09.9052156
Produced by FastQC (version 0.11.5)