##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512213_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2705492 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.94996141182454 31.0 31.0 34.0 28.0 34.0 2 31.14186070407896 31.0 31.0 34.0 28.0 34.0 3 31.23448821877869 31.0 31.0 34.0 27.0 34.0 4 34.83943844594624 37.0 35.0 37.0 32.0 37.0 5 34.45503701360048 37.0 35.0 37.0 30.0 37.0 6 34.38584220541033 37.0 35.0 37.0 30.0 37.0 7 34.21496311946219 37.0 35.0 37.0 30.0 37.0 8 34.119382722255324 37.0 35.0 37.0 30.0 37.0 9 35.62865866910713 39.0 35.0 39.0 30.0 39.0 10 35.43933376997603 39.0 35.0 39.0 30.0 39.0 11 35.424354239450714 39.0 35.0 39.0 30.0 39.0 12 35.2477375649235 39.0 35.0 39.0 29.0 39.0 13 35.228202855524984 39.0 35.0 39.0 29.0 39.0 14 36.254422855436275 39.0 36.0 41.0 29.0 41.0 15 36.18732821978406 39.0 36.0 41.0 29.0 41.0 16 36.0876328593838 39.0 36.0 41.0 28.0 41.0 17 36.01216784230003 39.0 36.0 41.0 27.0 41.0 18 35.94624822398292 39.0 36.0 41.0 27.0 41.0 19 35.9653035381365 39.0 36.0 41.0 27.0 41.0 20 35.8407298931211 39.0 36.0 41.0 27.0 41.0 21 35.73266562976346 39.0 36.0 41.0 27.0 41.0 22 35.82452951256185 39.0 36.0 41.0 27.0 41.0 23 35.71912058878755 39.0 36.0 41.0 27.0 41.0 24 35.596251994092015 39.0 36.0 41.0 27.0 41.0 25 35.148004133813735 39.0 35.0 40.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24120553723970417 1101 2 0.2656066551785514 1101 3 0.30118809336065766 1101 4 0.275340504239729 1101 5 0.39174946563549895 1101 6 0.3681210125081762 1101 7 0.30791534576496105 1101 8 0.13190898675750162 1101 9 0.30544396379410443 1101 10 0.45334470961073947 1101 11 0.45875437810515507 1101 12 0.41340574658027407 1101 13 0.2579594882462857 1101 14 0.31343486866335013 1101 15 -0.024474852203375974 1101 16 0.243534376133951 1101 17 0.09054484264989071 1101 18 0.2722513118617371 1101 19 0.13579790359202804 1101 20 0.06653802377047668 1101 21 0.23032448307122166 1101 22 0.026629383384594973 1101 23 0.04405454889933935 1101 24 -0.29842133142591365 1101 25 -0.15390166023648533 1102 1 0.37105832985047016 1102 2 0.40922766831176105 1102 3 0.3748206521714543 1102 4 0.403283449349658 1102 5 0.5767954078108986 1102 6 0.6527941255114911 1102 7 0.49671363315611927 1102 8 0.5458816619872167 1102 9 0.682535415181988 1102 10 0.5873010284638696 1102 11 0.637180798464037 1102 12 0.7074885868169574 1102 13 0.6563960110730562 1102 14 0.8706028101652521 1102 15 1.0029701855606135 1102 16 0.7916245286331076 1102 17 0.9307310167009817 1102 18 0.9440726425010837 1102 19 0.9247164584580503 1102 20 1.0524306489689152 1102 21 0.9384232948285671 1102 22 0.9668203681346768 1102 23 0.9745423537773874 1102 24 1.0187143783927226 1102 25 0.9266727372234627 1103 1 0.31466572514897706 1103 2 0.26373230764130184 1103 3 0.34646663564024394 1103 4 0.4021207389681507 1103 5 0.25081592984099643 1103 6 0.23404110361570218 1103 7 0.33661263468337665 1103 8 0.2991529841001963 1103 9 0.45419598049715404 1103 10 0.36625635429139436 1103 11 0.261188657311763 1103 12 0.42040963763085415 1103 13 0.44817491958347944 1103 14 0.4489019529061764 1103 15 0.6029080208471456 1103 16 0.55736025952897 1103 17 0.3071514036571372 1103 18 0.4606683185999003 1103 19 0.35260692576785146 1103 20 0.4459992872547076 1103 21 0.5038827381090272 1103 22 0.6851106082746625 1103 23 0.9007420943740172 1103 24 0.5588720158266227 1103 25 0.6188514953106292 1104 1 0.23413947137015256 1104 2 0.283461828172598 1104 3 0.3417662047761141 1104 4 0.3055434913980051 1104 5 0.34424021840811037 1104 6 0.29264026574976754 1104 7 0.2694891038942657 1104 8 0.2572887696852675 1104 9 0.46060480478541876 1104 10 0.4591262911904508 1104 11 0.4317470208334271 1104 12 0.4995306986691972 1104 13 0.623460708225906 1104 14 0.7152935068357777 1104 15 0.7568022779309018 1104 16 0.7874918155167023 1104 17 0.9065250175915409 1104 18 0.8396666377972934 1104 19 0.6513025513560251 1104 20 0.7248052164462351 1104 21 0.810646663640668 1104 22 0.8034237506000181 1104 23 0.7659311361972172 1104 24 0.8361943048799318 1104 25 0.7527862586932983 1105 1 -0.009007740677827059 1105 2 0.09410751193599864 1105 3 0.11170251240619322 1105 4 0.0694981476098917 1105 5 0.028918155708048232 1105 6 0.17676879405325252 1105 7 0.20352765390597938 1105 8 0.06865476677135973 1105 9 0.06569406405295553 1105 10 0.21047037952214964 1105 11 0.21928338025616512 1105 12 0.11288257767144927 1105 13 0.10429183722000346 1105 14 0.10705658290739706 1105 15 0.1001778875975674 1105 16 0.20001292074002208 1105 17 0.01055650913426831 1105 18 0.15097820675400442 1105 19 0.30954102218913704 1105 20 0.3887371560756989 1105 21 0.4145874893784125 1105 22 0.3529172538866163 1105 23 0.32678450624375444 1105 24 0.37266401619886125 1105 25 0.36332463310267826 1106 1 0.07039509264877353 1106 2 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1107 13 0.21510666584497784 1107 14 0.2647922542284533 1107 15 0.15094277068183004 1107 16 0.3027087370241688 1107 17 0.2025647319953947 1107 18 0.2907823822927966 1107 19 0.39110647308910274 1107 20 0.2138517168198817 1107 21 0.18404973257754875 1107 22 0.1814522828170908 1107 23 0.2681966207358144 1107 24 0.12417922718361041 1107 25 -0.1102536198173496 1108 1 -0.27548435823067763 1108 2 -0.15571738663329526 1108 3 -0.09467427597732225 1108 4 -0.15709271294958427 1108 5 -0.09798340312175924 1108 6 -0.012070625122490242 1108 7 -0.10855968327104648 1108 8 -0.10462411428779461 1108 9 -0.025888788819138142 1108 10 -0.014407903629674479 1108 11 0.017186433854284644 1108 12 -0.006594253419727636 1108 13 0.04523300741005443 1108 14 -0.04107442285813789 1108 15 -0.01730876625679656 1108 16 0.11921962584359136 1108 17 0.15895657319576628 1108 18 0.053740858900063415 1108 19 0.11160220092071427 1108 20 0.031613517382552914 1108 21 0.22537384427911888 1108 22 0.1787751441279113 1108 23 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-0.2924364078667523 2313 10 -0.35057192479369803 2313 11 -0.36153049736264364 2313 12 -0.291223883049355 2313 13 -0.18648656930952257 2313 14 -0.3366167509004683 2313 15 -0.3250733165213475 2313 16 -0.25067455405059036 2313 17 -0.4067048024656046 2313 18 -0.16867977602056783 2313 19 -0.13400626468774846 2313 20 -0.2975928318237919 2313 21 -0.24579017688490268 2313 22 -0.121171945819178 2313 23 -0.08617193193689587 2313 24 -0.11371846654012074 2313 25 -0.07441559751871551 2314 1 -0.3011597712530296 2314 2 -0.29864903548331156 2314 3 -0.36049465174667716 2314 4 -0.2636281842809751 2314 5 -0.3859101928852837 2314 6 -0.3065911952553009 2314 7 -0.41405207070220484 2314 8 -0.4735470167821276 2314 9 -0.45371758700508025 2314 10 -0.5224756069014589 2314 11 -0.48560592118296597 2314 12 -0.504281328249661 2314 13 -0.49219734568356444 2314 14 -0.6694101857347192 2314 15 -0.6286110014365889 2314 16 -0.5581062568904542 2314 17 -0.5890892641868035 2314 18 -0.4890960377633604 2314 19 -0.4457908991764654 2314 20 -0.6110039105409939 2314 21 -0.5907785690671687 2314 22 -0.4015884775112184 2314 23 -0.5411039047260147 2314 24 -0.5020202158112639 2314 25 -0.5387215579981444 2315 1 -0.231889764926958 2315 2 -0.2402438230855104 2315 3 -0.291489125287157 2315 4 -0.2844808296807315 2315 5 -0.2720928743927189 2315 6 -0.3841838696178783 2315 7 -0.4169278831086487 2315 8 -0.3781883785090301 2315 9 -0.4261769151027508 2315 10 -0.4237862768445595 2315 11 -0.5075840681278478 2315 12 -0.5243073928379829 2315 13 -0.5276844353945549 2315 14 -0.5875016808225055 2315 15 -0.4471501953058521 2315 16 -0.6947296027947019 2315 17 -0.5335897562688174 2315 18 -0.7100439479012906 2315 19 -0.6540541729628373 2315 20 -0.6352327356622922 2315 21 -0.5258721556253576 2315 22 -0.41758333607459974 2315 23 -0.6905863657144238 2315 24 -0.33902036429688565 2315 25 -0.4011189217772042 2316 1 -1.0712929151559862 2316 2 -1.088619852188046 2316 3 -1.0285847183268508 2316 4 -1.0454629087436373 2316 5 -1.163735759902039 2316 6 -1.0384176156953657 2316 7 -1.2192812206169492 2316 8 -1.1716690744038232 2316 9 -1.3249478896168156 2316 10 -1.480196518568036 2316 11 -1.5963759301776221 2316 12 -1.7187008524045027 2316 13 -1.498227833779218 2316 14 -1.7758876933439964 2316 15 -1.8136049954083617 2316 16 -1.796623376331901 2316 17 -1.7028164507346162 2316 18 -1.6692740069447467 2316 19 -1.6596051957435307 2316 20 -1.8452320228640033 2316 21 -1.6502969316454639 2316 22 -1.8047926586277043 2316 23 -1.612851893261528 2316 24 -1.7604230028385714 2316 25 -1.7231646910647669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36611.0 3 5.0 4 641.0 5 6104.0 6 8054.0 7 6361.0 8 5291.0 9 4746.0 10 4452.0 11 4362.0 12 4119.0 13 4011.0 14 4036.0 15 4175.0 16 4202.0 17 4291.0 18 4662.0 19 5265.0 20 6295.0 21 8356.0 22 10756.0 23 13665.0 24 16862.0 25 20267.0 26 23894.0 27 26908.0 28 32131.0 29 39437.0 30 50358.0 31 65418.0 32 84085.0 33 109474.0 34 142735.0 35 187216.0 36 269870.0 37 461428.0 38 1024949.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87218122428554 19.45350136073387 13.577974635519702 25.096342779460883 2 14.997420062598597 20.42755994103845 40.51130071720782 24.06371927915514 3 18.778654677226914 24.362703715257705 29.996614294183832 26.86202731333155 4 12.960156599982554 17.050577122386613 37.55579391844441 32.433472359186425 5 13.424209898540312 37.61591735034225 35.62454776473303 13.335324986384414 6 32.3124222877022 36.968765754990216 17.641005776398526 13.077806180909054 7 28.364637559453143 31.494973927108266 21.860940634827234 18.279447878611357 8 25.198448193526353 37.43038234820136 20.115786703490528 17.255382754781756 9 26.233749720938004 15.522426235228195 20.213107264778458 38.030716779055346 10 15.31507023491476 29.0678368296783 33.896459497939745 21.720633437467196 11 34.50958273023909 22.311246900748554 24.421694834063455 18.757475534948913 12 23.457618799094583 24.424984439059514 31.45487031563945 20.66252644620646 13 29.113115100691484 21.762030713822107 25.80987118054683 23.31498300493958 14 21.041200639292224 22.083377071527103 27.264467978467504 29.610954310713172 15 22.981032655058673 31.035759854399863 23.18221602577276 22.8009914647687 16 22.30995323586246 28.14493629994101 27.515882508615807 22.029227955580723 17 21.76347222612375 28.65397495169086 27.719468399832635 21.863084422352756 18 21.703224404285802 27.3916167558433 29.753146562621513 21.152012277249387 19 23.67277375057845 26.77830871427452 27.310633333974003 22.238284201173023 20 23.6770613256295 27.30460855179021 27.90631057123806 21.112019551342232 21 22.979517219049253 26.696031627519133 27.137097429968378 23.187353723463236 22 23.32592371369052 27.808694315119027 27.251050825506045 21.614331145684407 23 22.500454630802828 27.56844226484499 27.718877010170424 22.212226094181762 24 23.24667749895398 26.650975127629284 28.263768660191936 21.838578713224805 25 22.858309876335568 27.197192183506115 27.979137287662574 21.965360652495743 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 477.0 1 477.0 2 571.5 3 666.0 4 666.0 5 666.0 6 1619.5 7 2573.0 8 2573.0 9 2573.0 10 3748.0 11 4923.0 12 4923.0 13 4923.0 14 7933.5 15 10944.0 16 10944.0 17 10944.0 18 19968.5 19 28993.0 20 28993.0 21 28993.0 22 46960.0 23 64927.0 24 64927.0 25 64927.0 26 94893.0 27 124859.0 28 124859.0 29 124859.0 30 163074.0 31 201289.0 32 201289.0 33 201289.0 34 227707.5 35 254126.0 36 254126.0 37 254126.0 38 274755.0 39 295384.0 40 295384.0 41 295384.0 42 316362.0 43 337340.0 44 337340.0 45 337340.0 46 344220.5 47 351101.0 48 351101.0 49 351101.0 50 340318.0 51 329535.0 52 329535.0 53 329535.0 54 296402.5 55 263270.0 56 263270.0 57 263270.0 58 227680.0 59 192090.0 60 192090.0 61 192090.0 62 157461.0 63 122832.0 64 122832.0 65 122832.0 66 95377.5 67 67923.0 68 67923.0 69 67923.0 70 50087.0 71 32251.0 72 32251.0 73 32251.0 74 22648.0 75 13045.0 76 13045.0 77 13045.0 78 8980.0 79 4915.0 80 4915.0 81 4915.0 82 3207.0 83 1499.0 84 1499.0 85 1499.0 86 959.5 87 420.0 88 420.0 89 420.0 90 257.5 91 95.0 92 95.0 93 95.0 94 54.5 95 14.0 96 14.0 97 14.0 98 7.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.43337773684047115 2 0.0 3 0.0 4 0.0 5 0.032193774736720714 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006911866677114551 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2705492.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.18530224686349 #Duplication Level Percentage of deduplicated Percentage of total 1 82.82311745372995 58.129655315175114 2 11.5910082134974 16.270368296203845 3 2.8769165926140063 6.05751781774892 4 1.0895617801167166 3.058848914164895 5 0.5180986077492247 1.8181453689279252 6 0.2804992830103945 1.1812156174867825 7 0.17961488851695076 0.8824427667019216 8 0.11856590841722596 0.6657267294749553 9 0.08864605943518379 0.5599485427006619 >10 0.3948548088087592 4.694129271027682 >50 0.02108382210980566 1.0341208472434 >100 0.014351161826461525 2.02134729051772 >500 0.0020510769508076195 1.0282110703930005 >1k 0.001472568067090908 1.9534386223130782 >5k 1.5777515004545444E-4 0.6448835299201049 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6628 0.24498316757173927 No Hit GTCCTACAGTGGACATTTCTAAATT 5589 0.20657980138178195 No Hit CTGTAGGACGTGGAATATGGCAAGA 5254 0.19419758032919707 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4480 0.16558910541964272 No Hit GGTATCAACGCAGAGTACTTTTTTT 4377 0.16178203446914646 No Hit CTTTAGGACGTGAAATATGGCGAGG 4305 0.15912078098918792 No Hit TATCAACGCAGAGTACTTTTTTTTT 4276 0.15804888722642682 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 7.392370777662621E-5 0.0 0.0 0.0 6 0.0 7.392370777662621E-5 0.0 0.0 0.0 7 0.0 7.392370777662621E-5 0.0 0.0 0.0 8 0.0 7.392370777662621E-5 0.0 0.0 0.0 9 0.0 7.392370777662621E-5 0.0 3.6961853888313105E-5 0.0 10 0.0 7.392370777662621E-5 0.0 3.6961853888313105E-5 0.0 11 0.0 7.392370777662621E-5 0.0 3.6961853888313105E-5 0.0 12 0.0 7.392370777662621E-5 0.0 3.6961853888313105E-5 0.0 13 0.0 7.392370777662621E-5 0.0 3.6961853888313105E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCACGA 35 0.002175136 16.280804 12 TTACGCT 40 0.0052793478 14.250444 4 TAGGACC 840 0.0 11.649172 4 CTACACT 405 0.0 10.790459 4 TATACCG 80 3.771533E-4 10.687834 5 TGTAGGA 2760 0.0 10.670623 2 GTCCTAA 1430 0.0 10.605617 1 GGTATCA 1785 0.0 10.580394 1 TAGGACT 470 0.0 10.510965 4 GTAGGAC 2745 0.0 10.486666 3 GTCCTAC 2630 0.0 10.481343 1 GCCGGTC 100 2.4081777E-5 10.446849 12 AGGCGTG 210 0.0 10.401625 7 AAGGCGT 220 0.0 10.360511 6 TCCAACG 355 0.0 10.165948 18 CTGTAGG 2735 0.0 10.113827 1 TTAAGAC 275 0.0 10.018493 3 GGCGAGG 1065 0.0 9.988337 19 GCGTGCG 105 4.1208965E-5 9.94938 9 GGACGTG 2560 0.0 9.905215 6 >>END_MODULE