FastQCFastQC Report
Thu 26 May 2016
SRR1512212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780226
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT77740.27961755627060536No Hit
CTGTAGGACGTGGAATATGGCAAGA68550.24656268950797525No Hit
GTCCTAAAGTGTGTATTTCTCATTT59600.21437106192086544No Hit
CTTTAGGACGTGAAATATGGCGAGG54450.19584738794615977No Hit
GTATCAACGCAGAGTACTTTTTTTT51790.18627982041747687No Hit
GTCCTACAGTGTGCATTTCTCATTT33420.12020605519119669No Hit
GGTATCAACGCAGAGTACTTTTTTT30940.11128591704415397No Hit
TATCAACGCAGAGTACTTTTTTTTT30180.10855232632167314No Hit
CTGAAGGACCTGGAATATGGCGAGA29660.10668197477471256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12500.012.7379344
CGGTAGG1051.05233994E-712.6533571
TCCAACG4200.012.11472618
CGTAGGA956.0424427E-611.8337052
GGACGTA803.121615E-511.7771476
CCAACGT2050.011.03129619
TGTGCGA600.006252539310.99160910
ATTTAGA10050.010.9827061
TGTAGAA12600.010.8689092
AGGACGT39400.010.8084775
GTCCTAC37050.010.6475761
GGCGAGG18150.010.64256719
GGACGTG38550.010.631516
TTAGGAC22450.010.5756353
GACGTGG20900.010.5485287
TAGAAAT11500.010.4865924
CACCTTT8900.010.48090414
GATATAC9500.010.4351761
AGGACCT25100.010.4350215
GGACCTG23750.010.35397156
Produced by FastQC (version 0.11.5)