FastQCFastQC Report
Thu 26 May 2016
SRR1512212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780226
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT87420.31443486968325596No Hit
CTGTAGGACGTGGAATATGGCAAGA77520.27882625369304515No Hit
GTATCAACGCAGAGTACTTTTTTTT73970.2660575075551412No Hit
GTCCTAAAGTGTGTATTTCTCATTT66270.23836191734053275No Hit
CTTTAGGACGTGAAATATGGCGAGG64030.23030501836901027No Hit
TATCAACGCAGAGTACTTTTTTTTT46430.16700081216419096No Hit
GGTATCAACGCAGAGTACTTTTTTT45480.16358382376108993No Hit
GTCCTACAGTGTGCATTTCTCATTT36600.13164397426684019No Hit
CTGAAGGACCTGGAATATGGCGAGA34560.12430644127491794No Hit
CTGTAGGACCTGGAATATGGCGAGA29730.10693375286757263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2650.013.97758619
TCCAACG3800.012.74584818
TAGGACC11800.012.7210024
CGTGCGC604.1050932E-412.66254210
GGCGAGG18300.011.88496119
GAGCCGT658.048875E-411.68856
ACCGTCC600.005891911.0797248
GTGTTAC2000.010.9719571
TAGTACG700.001491395410.8577084
GGTATCA18900.010.8028481
TGTAGGA40550.010.8008092
AGGACGT41900.010.7022285
TAGGACT5000.010.6405534
GTAGGAC39850.010.6329253
GTCCTAA20200.010.5799331
AAATGTC8750.010.5279997
GGACGTG42100.010.4894576
ACTGTTC12550.010.3671428
CTGTAGG41050.010.3659231
AGGACCT23700.010.3423115
Produced by FastQC (version 0.11.5)