FastQCFastQC Report
Thu 26 May 2016
SRR1512211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500666
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50940.203705732792784No Hit
GTCCTACAGTGGACATTTCTAAATT36520.1460410946523846No Hit
CTGTAGGACGTGGAATATGGCAAGA34370.1374433850822141No Hit
GGTATCAACGCAGAGTACTTTTTTT31170.12464679409405334No Hit
GTCCTAAAGTGTGTATTTCTCATTT30880.12348710303575128No Hit
TATCAACGCAGAGTACTTTTTTTTT30340.12132767830649914No Hit
CTTTAGGACGTGAAATATGGCGAGG26430.10569184369284024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2600.013.04928118
GTCCTAA10400.011.7731461
CGAACAG658.5906405E-411.597975
CCAACGA1800.011.51881119
GGTATCA16700.011.0587661
TAGGACC6400.011.042994
CTACACG700.001596223110.7695444
GGCGAGG8600.010.73952519
GTCCTAC18750.010.4482751
GTCCTAT2550.010.403461
GATATAC6400.010.3628221
CGAAATC1106.6560824E-610.28084213
TGTAGGA18300.010.2591492
GTGTAGG4350.010.0861121
TGTAGAA8500.010.0833532
TTAGGAC13050.010.0371323
TCCTATA3050.010.0361242
CTGGTCG857.110921E-49.9768689
TAGGACA7150.09.8846344
TCCTACA20350.09.8774852
Produced by FastQC (version 0.11.5)