FastQCFastQC Report
Thu 26 May 2016
SRR1512211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500666
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66660.2665689860221237No Hit
TATCAACGCAGAGTACTTTTTTTTT43690.1747134563352323No Hit
GGTATCAACGCAGAGTACTTTTTTT41170.1646361409320557No Hit
CTGTAGGACGTGGAATATGGCAAGA39400.1575580265417293No Hit
GTCCTACAGTGGACATTTCTAAATT38000.15195951798440896No Hit
GTCCTAAAGTGTGTATTTCTCATTT31640.12652629339543947No Hit
CTTTAGGACGTGAAATATGGCGAGG31040.12412693258515932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA658.045932E-411.6889246
TAGGACC5250.011.4003494
TTACACG700.001491572710.8574744
GGTATCA18550.010.745311
TCCAACG2150.010.60158318
CCAACGT1106.06685E-610.36105119
GAACAGT6550.010.14973456
GGACGTG20400.010.0559136
AGGACGT20800.010.0027095
GTCCTAA11550.09.8261091
TTAGGAC11900.09.8196283
GACGTGA10450.09.815347
TAGGACA5450.09.7617664
GTAGAAC7900.09.7408043
GATATAC7150.09.7371841
TGTAGGA21500.09.677042
GGCGAGG9850.09.64226419
GTAGGAC20550.09.5696353
AGCGTAC1106.553581E-59.5371061
GTATTAG2201.6370905E-119.5371061
Produced by FastQC (version 0.11.5)