FastQCFastQC Report
Thu 26 May 2016
SRR1512210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823873
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT60660.3325889467084605No Hit
CTGTAGGACGTGGAATATGGCAAGA50960.27940541912731864No Hit
GTCCTAAAGTGTGTATTTCTCATTT43790.24009347142043333No Hit
GTATCAACGCAGAGTACTTTTTTTT40440.22172596447230702No Hit
CTTTAGGACGTGAAATATGGCGAGG36670.20105566560829619No Hit
GTCCTACAGTGTGCATTTCTCATTT26970.14787213802715432No Hit
GGTATCAACGCAGAGTACTTTTTTT24490.13427470004764586No Hit
TATCAACGCAGAGTACTTTTTTTTT23430.128462891878985No Hit
TTGTAGAACAGTGTATATCAATGAG21500.11788101474170624No Hit
CTGAAGGACCTGGAATATGGCGAGA19820.10866984707816826No Hit
GTGTATATCAATGAGTTACAATGAA19420.10647671192018304No Hit
CTGTAGGACCTGGAATATGGCGAGA19180.1051608308253919No Hit
GATATACACTGTTCTACAAATCCCG19010.10422874838324817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG453.7679696E-516.766095
GCACCGT400.00554139214.1463876
TAGGACC9800.012.9915814
GGTATCA7350.012.9534091
TCCAACG3550.012.75237318
CCAACGA2200.012.43235619
CGGTAGG903.6511065E-612.3792321
GGCGAGG12200.011.441421519
GGCCTAC1704.3655746E-1111.3200471
TGTAGGA29000.011.2810132
GATATAC9350.011.1575581
ACGAAAT1201.4097714E-711.00334912
AGGACGT30100.010.934865
GGACGTG30050.010.9216716
GTCCTAC28700.010.9048611
CTGTAGG28200.010.882711
GTAGGAC31050.010.8432863
CACCTTT8500.010.76294114
TCCTACA30500.010.7262092
TCCCGTT2550.010.72507311
Produced by FastQC (version 0.11.5)