##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512210_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1823873 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.754259205547754 31.0 30.0 34.0 25.0 34.0 2 26.999632101577248 31.0 27.0 34.0 7.0 34.0 3 28.610465750630663 31.0 28.0 34.0 16.0 34.0 4 33.14292168369179 35.0 33.0 37.0 28.0 37.0 5 33.543084962604304 35.0 35.0 37.0 28.0 37.0 6 33.75180618387355 37.0 35.0 37.0 30.0 37.0 7 33.759746977996826 37.0 35.0 37.0 29.0 37.0 8 33.76324886656034 37.0 35.0 37.0 29.0 37.0 9 35.31422911573339 39.0 35.0 39.0 30.0 39.0 10 35.179602965776674 39.0 35.0 39.0 29.0 39.0 11 35.20552582334406 39.0 35.0 39.0 29.0 39.0 12 35.0907897644189 39.0 35.0 39.0 29.0 39.0 13 35.10533902305698 39.0 35.0 39.0 29.0 39.0 14 36.20063184223901 40.0 36.0 41.0 29.0 41.0 15 36.14914141499984 40.0 36.0 41.0 29.0 41.0 16 36.10311518400678 40.0 36.0 41.0 28.0 41.0 17 36.088600467247446 40.0 36.0 41.0 29.0 41.0 18 36.04278203581061 40.0 36.0 41.0 28.0 41.0 19 36.06375937359674 40.0 36.0 41.0 28.0 41.0 20 35.98563441643141 40.0 36.0 41.0 27.0 41.0 21 35.910111614131026 40.0 36.0 41.0 27.0 41.0 22 35.81757117957226 40.0 36.0 41.0 27.0 41.0 23 35.71313901790311 40.0 36.0 41.0 27.0 41.0 24 35.617324780837265 40.0 36.0 41.0 27.0 41.0 25 35.498139947244134 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3091368089174509 1101 2 3.429086347605679 1101 3 1.9248137514471644 1101 4 0.7315607151756964 1101 5 0.14756689257315259 1101 6 0.282575222582949 1101 7 0.03452751360517681 1101 8 0.11851812355735802 1101 9 0.11238694862687737 1101 10 0.02645514360160206 1101 11 0.14977873957118248 1101 12 0.05795930295499829 1101 13 0.07976335208709884 1101 14 0.10559114560275162 1101 15 0.17525997289277484 1101 16 0.36100743149308556 1101 17 0.09031122703127181 1101 18 0.14832898750055534 1101 19 0.027049162479315214 1101 20 -0.018294179558914436 1101 21 0.18406556658072049 1101 22 0.09584822493346223 1101 23 0.17080740031238406 1101 24 0.041584181301786316 1101 25 0.11215498417983838 1102 1 1.2651220419330755 1102 2 4.091506081766877 1102 3 2.519096921330899 1102 4 1.6319158286162434 1102 5 1.1933547453775333 1102 6 1.14313805532948 1102 7 1.1212929209241551 1102 8 0.8528111086883641 1102 9 1.0324423110328311 1102 10 0.9390726991087561 1102 11 1.0304300198268237 1102 12 1.1419130279240477 1102 13 1.0756412239429807 1102 14 1.2824406717965076 1102 15 1.3169314874460838 1102 16 1.1658155241093127 1102 17 1.3227252540121768 1102 18 1.423562857823498 1102 19 1.407282191889216 1102 20 1.2839955852887144 1102 21 1.449421879356258 1102 22 1.1506346743780398 1102 23 1.3122305008828263 1102 24 1.3159583903610397 1102 25 1.2960670770607479 1103 1 1.2896561101240813 1103 2 4.026008250288893 1103 3 2.5526016789698858 1103 4 1.5456731070937977 1103 5 1.2388775940616696 1103 6 0.9677117249931158 1103 7 0.8035752220701369 1103 8 1.032073592711221 1103 9 0.9753902446552942 1103 10 1.0913816015198066 1103 11 0.9857532770889108 1103 12 1.143257293084588 1103 13 1.0957254521785558 1103 14 1.2438906865446384 1103 15 1.1251109667823087 1103 16 1.276521850512843 1103 17 1.3646266163028358 1103 18 1.1569540002030791 1103 19 1.3368128734715228 1103 20 1.3183248728991899 1103 21 1.2715539133971347 1103 22 1.3513395805908317 1103 23 1.317396717822426 1103 24 1.4642948553970356 1103 25 1.4175308021118198 1104 1 1.1762005027543125 1104 2 4.267697496432049 1104 3 2.541417487071925 1104 4 1.5646551139373415 1104 5 1.2115331038532489 1104 6 0.9785520517929243 1104 7 0.8807684443066393 1104 8 0.8306873508000763 1104 9 0.9632945243736302 1104 10 0.834216247875105 1104 11 0.7976574348345054 1104 12 0.7680762398064616 1104 13 0.9305073932439498 1104 14 1.1051450672171867 1104 15 0.7024190829787642 1104 16 0.9069185995038467 1104 17 1.0873109857146872 1104 18 1.0995192719517988 1104 19 1.0296011266259342 1104 20 1.116751860237983 1104 21 1.2048446288761312 1104 22 1.0275240229685139 1104 23 1.1988751580525232 1104 24 1.132596265271161 1104 25 1.0589628164697586 1105 1 1.1908306842035579 1105 2 4.103754256671714 1105 3 2.5372329469569443 1105 4 1.6621400021952226 1105 5 1.289733897859577 1105 6 1.083808538371791 1105 7 0.8024121858326083 1105 8 0.8288345068566088 1105 9 1.0047948702414402 1105 10 0.9216425309038598 1105 11 1.1129748559010793 1105 12 1.0111479143429492 1105 13 1.0673499803243587 1105 14 1.1185407416215085 1105 15 1.1364468964928989 1105 16 1.0151576204352182 1105 17 1.07839507140757 1105 18 1.1124794291713371 1105 19 1.2746358786221919 1105 20 1.2473214289622163 1105 21 1.3161552918427049 1105 22 1.3626994567658386 1105 23 1.056395494375792 1105 24 1.189989738255285 1105 25 1.1273763909553836 1106 1 0.27922125864325764 1106 2 3.0810622175371094 1106 3 1.486720942218625 1106 4 0.6345267946736897 1106 5 0.24505187331487122 1106 6 0.12903883861930154 1106 7 0.03199788175966489 1106 8 -0.18165364326986122 1106 9 -0.06416343309249584 1106 10 -0.1256792591157847 1106 11 -0.059387212549154356 1106 12 -0.013178355887959015 1106 13 -0.18127783610923842 1106 14 -0.003721208099058515 1106 15 -0.25695885197950474 1106 16 -0.1287014471700587 1106 17 0.06893935322888467 1106 18 -0.15450502729399318 1106 19 -0.3175219856564766 1106 20 -0.09146133377133481 1106 21 -0.40488621463852326 1106 22 -0.33448281909453925 1106 23 -0.24912720146704004 1106 24 -0.29239670102277415 1106 25 -0.44701386288703304 1107 1 0.9013763263886965 1107 2 3.8786672202770127 1107 3 2.297599698840056 1107 4 1.5041700163694145 1107 5 0.9928830523325871 1107 6 0.8627949765384813 1107 7 0.6483524144182269 1107 8 0.7031632667646264 1107 9 0.66060125762084 1107 10 0.6509684431266365 1107 11 0.6083168564749002 1107 12 0.44771947260488076 1107 13 0.6132560580982869 1107 14 0.7297176920717874 1107 15 0.5823745995457088 1107 16 0.7077489283577734 1107 17 0.4380636051280149 1107 18 0.5547233409059444 1107 19 0.7189047514955291 1107 20 0.6352489564434478 1107 21 0.7781981098948876 1107 22 0.6827530489173625 1107 23 0.8884553985394064 1107 24 0.8543739744975412 1107 25 0.9294826074736662 1108 1 0.8157641542536744 1108 2 3.4866403639147343 1108 3 2.1751405718866152 1108 4 1.1696500115582964 1108 5 0.7874675347505189 1108 6 0.5623125757893206 1108 7 0.6062400747531171 1108 8 0.49238141671694535 1108 9 0.5187129357568807 1108 10 0.276196112224639 1108 11 0.4854442738482021 1108 12 0.6068451373556911 1108 13 0.5155505759982333 1108 14 0.7148880572873821 1108 15 0.632999830436539 1108 16 0.5065130717635355 1108 17 0.6664769233406815 1108 18 0.7636387339143553 1108 19 0.7844111648910612 1108 20 0.7478721340167525 1108 21 0.660118391345442 1108 22 0.5687370089850603 1108 23 0.8827110494582442 1108 24 0.8327260797923515 1108 25 0.7507924350465487 1109 1 0.8516007154210392 1109 2 3.442984225454232 1109 3 2.1464941332212533 1109 4 1.216250303964216 1109 5 0.7855715669542747 1109 6 0.8054526590793856 1109 7 0.5261374719928611 1109 8 0.4209260781365316 1109 9 0.5994878983371734 1109 10 0.5756487744002143 1109 11 0.4001756883672627 1109 12 0.564217633385617 1109 13 0.57307318531916 1109 14 0.53581441487853 1109 15 0.5490379474814588 1109 16 0.7029828025371927 1109 17 0.5856791504918561 1109 18 0.5984315623659242 1109 19 0.6221905092153222 1109 20 0.6645951022192378 1109 21 0.6647505051621607 1109 22 0.642616210147736 1109 23 0.7051988242405187 1109 24 0.7045578964238572 1109 25 0.6929331027139654 1110 1 0.7103858472633569 1110 2 3.541125570462871 1110 3 1.9657100652300201 1110 4 1.0366945889572605 1110 5 0.5347800368539453 1110 6 0.5404896151882213 1110 7 0.45840679190197164 1110 8 0.29292222749342756 1110 9 0.27610124799083025 1110 10 0.15250362799081785 1110 11 0.3036793623331562 1110 12 0.1879241425200675 1110 13 0.35849642326844844 1110 14 0.4132537372172962 1110 15 0.3692952739035462 1110 16 0.43529654878111046 1110 17 0.5416673171777333 1110 18 0.5456029143604866 1110 19 0.42529201933191985 1110 20 0.4141566559613068 1110 21 0.35818742057842456 1110 22 0.38499379286810864 1110 23 0.3763625074163528 1110 24 0.4979735456408747 1110 25 0.2820422654155408 1111 1 -0.6290375947579712 1111 2 1.6037609954697274 1111 3 0.35895804555398314 1111 4 -0.49572532629590427 1111 5 -0.8362440000060758 1111 6 -0.9640285040146921 1111 7 -1.0140779898422423 1111 8 -1.1740836734657591 1111 9 -1.3372609011740408 1111 10 -1.214496912797138 1111 11 -1.4180606783423997 1111 12 -1.3708852147112083 1111 13 -1.427711042299471 1111 14 -1.6988274432776294 1111 15 -1.7836570719290918 1111 16 -1.5660479842230899 1111 17 -1.5882020546073932 1111 18 -1.603039124143507 1111 19 -1.7583437988477044 1111 20 -1.8427302520028732 1111 21 -1.723843124539762 1111 22 -1.6940563847892918 1111 23 -1.8202710244116957 1111 24 -1.9273591287562226 1111 25 -1.8198178262574203 1112 1 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2315 18 -1.7840250864146796 2315 19 -2.0357269205748523 2315 20 -1.9194616121953985 2315 21 -1.8441496349003899 2315 22 -1.8498072497691709 2315 23 -1.9113606067265891 2315 24 -1.710296702355656 2315 25 -1.5391997731485887 2316 1 -1.2981580032625928 2316 2 0.2763930162484236 2316 3 -0.13401715192884112 2316 4 -0.84775222751189 2316 5 -0.8894647925883561 2316 6 -0.9968433167255455 2316 7 -1.2100188223155826 2316 8 -1.2480225681931358 2316 9 -1.0385377937111926 2316 10 -1.138315724081508 2316 11 -1.6868206787211122 2316 12 -1.1516740729874968 2316 13 -1.1235373142214016 2316 14 -1.4331640493187265 2316 15 -1.6686379627241692 2316 16 -1.5220106557877742 2316 17 -1.5455675406376344 2316 18 -1.881117031630339 2316 19 -1.8709154780907866 2316 20 -2.0232738938922665 2316 21 -1.9005055938088162 2316 22 -1.6582746747127004 2316 23 -1.723646615755591 2316 24 -1.7279088931654485 2316 25 -1.5103809402846124 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58286.0 3 1.0 4 527.0 5 2552.0 6 3014.0 7 2716.0 8 2541.0 9 2426.0 10 2328.0 11 2333.0 12 2269.0 13 2270.0 14 2274.0 15 2347.0 16 2553.0 17 2689.0 18 3113.0 19 3757.0 20 4893.0 21 6283.0 22 7619.0 23 9130.0 24 10811.0 25 12425.0 26 14348.0 27 16126.0 28 19487.0 29 23873.0 30 30337.0 31 39512.0 32 53229.0 33 72619.0 34 102773.0 35 150110.0 36 220882.0 37 291914.0 38 639506.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.6578347286242 19.227544900330233 13.482517697230016 25.632102673815556 2 14.68472462902805 20.508282061744946 42.60390776943419 22.203085539792816 3 18.79072720523852 24.010553366380226 29.93931046734065 27.2594089610406 4 11.586552353151781 16.8702535757698 39.23546211825056 32.30773195282786 5 12.342032586698744 37.97945361327242 36.82745454316173 12.851059256867117 6 30.79952562382496 38.01407886202353 18.510485128043737 12.675910386107775 7 27.146188614232457 31.554454517190837 22.548421287545928 18.750935581030774 8 23.37427002866976 39.52046003203074 20.82425695210138 16.28101298719812 9 25.452594561134468 15.805541284946923 20.91121476111549 37.83064939280312 10 14.25072445093884 29.789312943614828 35.258567985985266 20.70139461946107 11 33.65522005888707 22.29264788933179 25.85081942944244 18.201312622338705 12 23.01394182899602 24.40867301509323 33.16025756306391 19.417127592846835 13 28.673981137941073 22.503540542570672 26.307259332201312 22.515218987286943 14 19.435199735068256 23.361255706285437 28.234969016364143 28.968575542282167 15 21.64964336880912 33.11815022208235 23.677909591292813 21.554296817815715 16 20.48125061339249 28.694651436805085 30.07490104848309 20.749196901319337 17 20.205606759253676 29.517783613963715 29.369253291078863 20.90735633570375 18 19.884452842645413 28.05725374807188 32.26058866977651 19.797704739506194 19 22.650610204768686 27.03718690612091 28.211374624824682 22.10082826428572 20 22.32600625153177 28.362665602265068 29.477545859826865 19.8337822863763 21 21.430717705657994 27.511081526256802 27.962622192881064 23.09557857520414 22 21.607785340583977 29.56276818445498 28.52013172222533 20.309314752735713 23 20.910134174498676 28.824790179288623 29.073279749799735 21.191795896412966 24 22.145009390379318 27.27563847725061 30.161021015257656 20.418331117112423 25 21.479146435815174 28.149062832935158 29.650208006509697 20.72158272473997 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 243.0 1 243.0 2 314.5 3 386.0 4 386.0 5 386.0 6 1088.0 7 1790.0 8 1790.0 9 1790.0 10 2700.0 11 3610.0 12 3610.0 13 3610.0 14 6130.5 15 8651.0 16 8651.0 17 8651.0 18 15627.5 19 22604.0 20 22604.0 21 22604.0 22 37860.5 23 53117.0 24 53117.0 25 53117.0 26 78190.5 27 103264.0 28 103264.0 29 103264.0 30 137262.5 31 171261.0 32 171261.0 33 171261.0 34 187608.5 35 203956.0 36 203956.0 37 203956.0 38 213687.0 39 223418.0 40 223418.0 41 223418.0 42 229755.5 43 236093.0 44 236093.0 45 236093.0 46 234655.0 47 233217.0 48 233217.0 49 233217.0 50 217498.0 51 201779.0 52 201779.0 53 201779.0 54 174480.5 55 147182.0 56 147182.0 57 147182.0 58 123480.5 59 99779.0 60 99779.0 61 99779.0 62 79938.5 63 60098.0 64 60098.0 65 60098.0 66 45780.5 67 31463.0 68 31463.0 69 31463.0 70 22688.5 71 13914.0 72 13914.0 73 13914.0 74 9607.5 75 5301.0 76 5301.0 77 5301.0 78 3588.0 79 1875.0 80 1875.0 81 1875.0 82 1249.0 83 623.0 84 623.0 85 623.0 86 399.0 87 175.0 88 175.0 89 175.0 90 114.5 91 54.0 92 54.0 93 54.0 94 33.5 95 13.0 96 13.0 97 13.0 98 10.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 6.884470574431443 3 0.0 4 0.0 5 0.0 6 2.1931351579852326E-4 7 0.007675973052948314 8 0.0 9 0.0 10 0.004276613558071203 11 0.002357620294834125 12 4.934554105466772E-4 13 0.0 14 3.8379865264741574E-4 15 0.0 16 0.0 17 1.6448513684889246E-4 18 0.011075332547825424 19 0.003234874358028218 20 0.0 21 0.0064149203371068044 22 0.0019189932632370785 23 0.002960732463280064 24 0.010856019032026902 25 0.0072373460213512675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1823873.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.67101261190858 #Duplication Level Percentage of deduplicated Percentage of total 1 89.86722243375436 68.90210944615575 2 7.246305801524181 11.111632069968136 3 1.4232845609537048 3.2737400556964884 4 0.5198239598923208 1.5942171753950556 5 0.25315192637969436 0.9704707270093248 6 0.15165721462185833 0.6976627328975656 7 0.09648200235216742 0.5178160973415655 8 0.07557607977346315 0.4635595652375842 9 0.050177471127162164 0.34624417694618964 >10 0.2732027912232322 3.7511158944992524 >50 0.021899870883316925 1.1951300895721229 >100 0.016814787020773027 2.618194186186958 >500 0.0023809232180320695 1.2921617945034924 >1k 0.0018758788990555692 2.648491904500972 >5k 1.442983768504284E-4 0.6174540840895577 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6066 0.3325889467084605 No Hit CTGTAGGACGTGGAATATGGCAAGA 5096 0.27940541912731864 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4379 0.24009347142043333 No Hit GTATCAACGCAGAGTACTTTTTTTT 4044 0.22172596447230702 No Hit CTTTAGGACGTGAAATATGGCGAGG 3667 0.20105566560829619 No Hit GTCCTACAGTGTGCATTTCTCATTT 2697 0.14787213802715432 No Hit GGTATCAACGCAGAGTACTTTTTTT 2449 0.13427470004764586 No Hit TATCAACGCAGAGTACTTTTTTTTT 2343 0.128462891878985 No Hit TTGTAGAACAGTGTATATCAATGAG 2150 0.11788101474170624 No Hit CTGAAGGACCTGGAATATGGCGAGA 1982 0.10866984707816826 No Hit GTGTATATCAATGAGTTACAATGAA 1942 0.10647671192018304 No Hit CTGTAGGACCTGGAATATGGCGAGA 1918 0.1051608308253919 No Hit GATATACACTGTTCTACAAATCCCG 1901 0.10422874838324817 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.4828378949630814E-5 2 0.0 0.0 0.0 0.0 5.4828378949630814E-5 3 0.0 0.0 0.0 0.0 5.4828378949630814E-5 4 0.0 0.0 0.0 0.0 5.4828378949630814E-5 5 0.0 0.0 0.0 5.4828378949630814E-5 5.4828378949630814E-5 6 0.0 0.0 0.0 5.4828378949630814E-5 5.4828378949630814E-5 7 0.0 0.0 0.0 5.4828378949630814E-5 5.4828378949630814E-5 8 0.0 0.0 0.0 5.4828378949630814E-5 5.4828378949630814E-5 9 0.0 0.0 0.0 1.0965675789926163E-4 5.4828378949630814E-5 10 0.0 0.0 0.0 1.6448513684889243E-4 5.4828378949630814E-5 11 0.0 0.0 0.0 1.6448513684889243E-4 5.4828378949630814E-5 12 0.0 0.0 0.0 1.6448513684889243E-4 1.0965675789926163E-4 13 0.0 0.0 0.0 1.6448513684889243E-4 1.0965675789926163E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTACCG 45 3.7679696E-5 16.76609 5 GCACCGT 40 0.005541392 14.146387 6 TAGGACC 980 0.0 12.991581 4 GGTATCA 735 0.0 12.953409 1 TCCAACG 355 0.0 12.752373 18 CCAACGA 220 0.0 12.432356 19 CGGTAGG 90 3.6511065E-6 12.379232 1 GGCGAGG 1220 0.0 11.4414215 19 GGCCTAC 170 4.3655746E-11 11.320047 1 TGTAGGA 2900 0.0 11.281013 2 GATATAC 935 0.0 11.157558 1 ACGAAAT 120 1.4097714E-7 11.003349 12 AGGACGT 3010 0.0 10.93486 5 GGACGTG 3005 0.0 10.921671 6 GTCCTAC 2870 0.0 10.904861 1 CTGTAGG 2820 0.0 10.88271 1 GTAGGAC 3105 0.0 10.843286 3 CACCTTT 850 0.0 10.762941 14 TCCTACA 3050 0.0 10.726209 2 TCCCGTT 255 0.0 10.725073 11 >>END_MODULE