FastQCFastQC Report
Thu 26 May 2016
SRR1512210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823873
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54650.2996370909597324No Hit
CTGTAGGACGTGGAATATGGCAAGA54110.29667635849645235No Hit
GTCCTACAGTGGACATTTCTAAATT49790.2729904987902118No Hit
CTTTAGGACGTGAAATATGGCGAGG41180.2257832645145797No Hit
GTCCTAAAGTGTGTATTTCTCATTT39210.21498207386150245No Hit
TATCAACGCAGAGTACTTTTTTTTT36680.20111049398724581No Hit
GGTATCAACGCAGAGTACTTTTTTT34980.1917896695658086No Hit
TTGTAGAACAGTGTATATCAATGAG24240.1329039905739051No Hit
CTGAAGGACCTGGAATATGGCGAGA23750.13021740000537319No Hit
GTCCTACAGTGTGCATTTCTCATTT23180.12709218240524423No Hit
GATATACACTGTTCTACAAATCCCG22510.12341868101561897No Hit
CTGTAGGACCTGGAATATGGCGAGA21230.11640064851006621No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19050.10444806189904669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8200.013.3228824
CTAGGAC1950.012.1792243
TCCAACG3050.012.14461418
GATATAC10950.012.1002431
TAGGACA7650.011.921314
GGTATCA11950.011.8055761
CCAACGA2100.011.76003619
TGTAGGA27800.011.6867972
CTGTAGG27550.011.2102891
GTAGGAC28300.011.1110673
AGGACGA1201.2758937E-711.0830945
AGGACCG600.005877267611.0830945
CACCTTT7600.010.87237914
CCTACAC2100.010.8569083
GTCGAGG1408.372808E-910.85541919
TGTCGAG1408.383722E-910.854526518
GTCTTAG1502.0172592E-910.8031651
ACCTTTT8000.010.68492515
ACTGTTC11300.010.6744198
GACGTGG14900.010.6450857
Produced by FastQC (version 0.11.5)