##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512210_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1823873 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.916365887317813 31.0 29.0 33.0 24.0 34.0 2 29.135187592557156 31.0 29.0 34.0 25.0 34.0 3 29.17518653985228 31.0 28.0 34.0 25.0 34.0 4 32.745937902474566 35.0 32.0 37.0 26.0 37.0 5 32.15977537909712 35.0 32.0 37.0 24.0 37.0 6 32.06846858306472 35.0 32.0 37.0 23.0 37.0 7 31.804258300879503 35.0 32.0 37.0 19.0 37.0 8 31.621749431018497 35.0 32.0 37.0 18.0 37.0 9 32.82147441186969 37.0 32.0 39.0 18.0 39.0 10 32.496820776446604 37.0 32.0 39.0 17.0 39.0 11 32.531480536199616 37.0 32.0 39.0 17.0 39.0 12 32.23054565751014 37.0 32.0 39.0 16.0 39.0 13 32.20520178762447 37.0 32.0 39.0 15.0 39.0 14 32.81286197010428 38.0 32.0 40.0 11.0 41.0 15 32.722269587849595 38.0 32.0 40.0 10.0 41.0 16 32.55887334260664 37.0 32.0 40.0 10.0 41.0 17 32.43744602831447 37.0 31.0 40.0 10.0 41.0 18 32.31792454847459 37.0 31.0 40.0 10.0 41.0 19 32.33673945499495 37.0 31.0 40.0 8.0 41.0 20 32.15765406911556 37.0 31.0 40.0 2.0 41.0 21 31.98054743943246 37.0 31.0 40.0 2.0 41.0 22 32.04120571991581 37.0 31.0 40.0 2.0 41.0 23 31.866594878042495 37.0 31.0 40.0 2.0 41.0 24 31.641533703278682 37.0 31.0 40.0 2.0 41.0 25 31.080296160971734 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2412996096154849 1101 2 0.27443512487802124 1101 3 0.3964191559831427 1101 4 0.3384385847879159 1101 5 0.6120001119123728 1101 6 0.5838052600890435 1101 7 0.5340409254082594 1101 8 -0.0038431988561775654 1101 9 0.2656878920800523 1101 10 0.3925926879592154 1101 11 0.3645426442957671 1101 12 0.3791205556408528 1101 13 0.33735677111061335 1101 14 0.20811498674688522 1101 15 0.27459432016082985 1101 16 0.4154724520053321 1101 17 0.4970717243985163 1101 18 0.55900016477554 1101 19 0.2894219724405076 1101 20 0.080012562725706 1101 21 -0.06696391029429449 1101 22 -0.10913618107984746 1101 23 -4.902768498276089E-4 1101 24 -0.4293103626165369 1101 25 -0.49754535645637077 1102 1 0.5273721597325256 1102 2 0.6038899650907616 1102 3 0.5485758911451626 1102 4 0.7638881975543583 1102 5 0.5886464404309066 1102 6 0.6499470683553525 1102 7 0.5834358815842826 1102 8 0.6069797655139233 1102 9 0.9597300921291279 1102 10 0.7932621098797554 1102 11 0.8592242139633441 1102 12 1.1399866684996525 1102 13 1.094507426884931 1102 14 1.2188888748806406 1102 15 1.1942623111506592 1102 16 1.1702142365606534 1102 17 1.4669328496780167 1102 18 1.5112098361026085 1102 19 1.3378370581447143 1102 20 1.2778471427241058 1102 21 1.5277992692944018 1102 22 1.397487456572101 1102 23 1.3286502548829198 1102 24 1.6200233861186497 1102 25 1.4110482233793604 1103 1 0.42763953795116905 1103 2 0.37645515730811496 1103 3 0.42572884568813407 1103 4 0.547599975305431 1103 5 0.34396860523857953 1103 6 0.5757336355358404 1103 7 0.5609796616950611 1103 8 0.5963029353796578 1103 9 0.8296108507294804 1103 10 0.7591267329283582 1103 11 0.6019075005032164 1103 12 0.7754409110463811 1103 13 0.7779321622378461 1103 14 0.6801556304002645 1103 15 0.8206183201961821 1103 16 0.7675333534205038 1103 17 0.5315684615071135 1103 18 0.908364265459042 1103 19 1.06529535369733 1103 20 0.5446807728408842 1103 21 1.0290334696723953 1103 22 0.9514664486736564 1103 23 1.3069532908137482 1103 24 0.9676445666248874 1103 25 1.05608137937093 1104 1 0.49581440111300523 1104 2 0.5037914252473641 1104 3 0.5479340909513475 1104 4 0.5463380475699182 1104 5 0.8206339686846604 1104 6 0.7726915592985222 1104 7 0.7744158841123401 1104 8 0.8208413992637524 1104 9 0.9532626163937152 1104 10 0.9913535630094472 1104 11 1.1991404618923056 1104 12 1.2633718723004392 1104 13 1.3608186252245673 1104 14 1.507861247026888 1104 15 1.5715734102708652 1104 16 1.760454578636626 1104 17 1.6276998566356085 1104 18 1.758623403068249 1104 19 1.5711527675704176 1104 20 1.4952485994827782 1104 21 1.6752643488126573 1104 22 1.6778541063397512 1104 23 1.576421827293398 1104 24 1.7218956431431813 1104 25 1.772523086205105 1105 1 0.5507542981164413 1105 2 0.5523789465966438 1105 3 0.5506891163267227 1105 4 0.47023602387756824 1105 5 0.5256509749806995 1105 6 0.8617529276783529 1105 7 0.9036845890066552 1105 8 0.785248703168616 1105 9 0.8207283451455964 1105 10 0.9266072165598587 1105 11 0.9979558984847472 1105 12 0.7953943025188295 1105 13 0.8652398830133663 1105 14 0.9322787021656396 1105 15 1.1083884254300074 1105 16 1.049069758577268 1105 17 0.7894990951305658 1105 18 1.1566286910269028 1105 19 1.1050410422452615 1105 20 1.2914958930739076 1105 21 1.2899361860722358 1105 22 1.3455528692867773 1105 23 1.2994085218100544 1105 24 1.400709844686883 1105 25 1.4146687992046978 1106 1 -0.12954690031969562 1106 2 -0.14336852054892546 1106 3 -0.1490742394031379 1106 4 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0.4874399535450067 1107 16 0.6233777496530877 1107 17 0.30593912459232797 1107 18 0.6918650489017111 1107 19 0.5922767749139908 1107 20 0.41649749891144694 1107 21 0.49737506789544383 1107 22 0.23304521479450102 1107 23 0.17073757719217397 1107 24 0.4650338084934411 1107 25 0.2651637183347155 1108 1 0.24582880079827873 1108 2 0.2162517174359948 1108 3 0.19643315470011302 1108 4 0.1686451705780101 1108 5 0.16290253313928105 1108 6 0.19342237864604783 1108 7 0.1469251460985852 1108 8 0.21595925389539872 1108 9 0.16967929003381954 1108 10 0.26651660548363054 1108 11 0.17846387230289906 1108 12 0.26977293994200835 1108 13 0.31202515583503754 1108 14 0.151049272139943 1108 15 0.12857116826703674 1108 16 0.3399995268466611 1108 17 0.417944803432853 1108 18 0.3938891560522393 1108 19 0.4074438324842049 1108 20 0.38359072296361774 1108 21 0.36576729449921785 1108 22 0.39727732558773 1108 23 0.5185397112677954 1108 24 0.482644872352477 1108 25 0.24954798081570928 1109 1 0.1470082003016664 1109 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-0.9048264513399147 2315 9 -1.2262737673022635 2315 10 -1.0864050845091135 2315 11 -0.9642608555464065 2315 12 -1.413567162867185 2315 13 -1.2578029744752008 2315 14 -1.4651365446436984 2315 15 -1.3307728991261527 2315 16 -1.6062518216210897 2315 17 -1.4219572898153992 2315 18 -1.65581438827164 2315 19 -1.979077351633304 2315 20 -1.6290810253492474 2315 21 -1.5352205358383735 2315 22 -1.4481184045505664 2315 23 -1.3128053105622826 2315 24 -1.1434370102802056 2315 25 -1.0738986613071688 2316 1 -1.1348497571135496 2316 2 -1.150173827986361 2316 3 -1.2498895937158139 2316 4 -1.2480672786034006 2316 5 -1.3086097548876765 2316 6 -1.230890578416055 2316 7 -1.4682093324080512 2316 8 -1.243876051138237 2316 9 -1.7238055529016378 2316 10 -1.7768553072487947 2316 11 -1.7232044425342963 2316 12 -1.798289177773995 2316 13 -1.6970794602633177 2316 14 -2.027871847639325 2316 15 -1.9138271029852056 2316 16 -1.9615204526427732 2316 17 -1.9093684344286785 2316 18 -2.052406122854837 2316 19 -1.7938289214256251 2316 20 -1.842599317202911 2316 21 -1.8389147306750573 2316 22 -1.682316491941478 2316 23 -1.6279466333341404 2316 24 -1.4896066506791783 2316 25 -1.6011943063566427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73738.0 3 1.0 4 1311.0 5 10003.0 6 12097.0 7 9853.0 8 7801.0 9 7020.0 10 6462.0 11 6268.0 12 5816.0 13 5617.0 14 5465.0 15 5435.0 16 5614.0 17 5717.0 18 6193.0 19 7072.0 20 8607.0 21 11019.0 22 13852.0 23 16988.0 24 20564.0 25 23581.0 26 27224.0 27 30622.0 28 35548.0 29 42683.0 30 53379.0 31 66412.0 32 83311.0 33 104248.0 34 130949.0 35 170874.0 36 228479.0 37 307058.0 38 266992.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.00246551715598 22.21893242006469 15.150052584858093 22.62854947792124 2 15.672472809236169 22.921113476651062 40.498543484113206 20.907870229999567 3 20.332391564544242 26.17808367139598 30.596867216083577 22.892657547976203 4 14.30768480042196 19.528552700763704 37.75301240821044 28.410750090603898 5 12.832576952080622 39.147953657366145 36.08643312538562 11.933036265167615 6 31.23654991328892 39.25509067791453 17.558349731587672 11.950009677208886 7 27.600551134865203 32.29709524731163 22.299195174225396 17.803158443597773 8 24.173612965376424 40.20460854456423 20.1234406123672 15.498337877692142 9 25.954877340692033 15.818480782379035 20.766467840688467 37.46017403624046 10 14.66818139201578 31.054793837070893 34.63662217709238 19.64040259382095 11 34.4845282538861 22.598119496258786 25.313988419149798 17.60336383070532 12 23.378820784122578 25.34041569780352 32.604572796461156 18.67619072161274 13 29.440152905383215 22.773679965655504 26.115963118046047 21.670204010915235 14 20.021350170762986 24.011978904232915 27.95731939669045 28.009351528313648 15 22.356874628880412 33.64044535995653 23.3385219255946 20.664158085568456 16 21.070052574932575 29.564394012083078 29.262399300828513 20.103154112155835 17 20.758846696014473 30.33813209582027 28.576331795031777 20.32668941313348 18 20.507513406909364 28.727219493901163 31.66311470151705 19.102152397672427 19 23.327117622773077 27.572150034569294 28.033640500188334 21.067091842469296 20 23.06712144979393 28.825636434115754 28.961994612563487 19.145247503526836 21 22.183452466262725 27.78916075845193 27.839328725190843 22.188058050094497 22 22.409838842945753 29.593946508336927 28.391121530939927 19.60509311777739 23 21.525950545898755 29.223306666637427 28.793945631082867 20.456797156380954 24 22.844298917742627 27.91203115567805 29.513897075070467 19.72977285150885 25 22.238361015109966 28.738602548686426 29.16590587702459 19.857130559179016 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 238.0 1 238.0 2 382.0 3 526.0 4 526.0 5 526.0 6 1242.5 7 1959.0 8 1959.0 9 1959.0 10 3056.0 11 4153.0 12 4153.0 13 4153.0 14 6813.5 15 9474.0 16 9474.0 17 9474.0 18 17222.5 19 24971.0 20 24971.0 21 24971.0 22 40808.0 23 56645.0 24 56645.0 25 56645.0 26 82861.0 27 109077.0 28 109077.0 29 109077.0 30 142570.0 31 176063.0 32 176063.0 33 176063.0 34 192422.5 35 208782.0 36 208782.0 37 208782.0 38 216691.0 39 224600.0 40 224600.0 41 224600.0 42 230817.5 43 237035.0 44 237035.0 45 237035.0 46 233705.5 47 230376.0 48 230376.0 49 230376.0 50 213848.0 51 197320.0 52 197320.0 53 197320.0 54 169045.5 55 140771.0 56 140771.0 57 140771.0 58 117452.0 59 94133.0 60 94133.0 61 94133.0 62 75489.0 63 56845.0 64 56845.0 65 56845.0 66 43388.5 67 29932.0 68 29932.0 69 29932.0 70 21561.0 71 13190.0 72 13190.0 73 13190.0 74 9184.5 75 5179.0 76 5179.0 77 5179.0 78 3511.5 79 1844.0 80 1844.0 81 1844.0 82 1201.5 83 559.0 84 559.0 85 559.0 86 363.0 87 167.0 88 167.0 89 167.0 90 96.5 91 26.0 92 26.0 93 26.0 94 16.0 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37338126064698585 2 0.0 3 0.0 4 0.0 5 0.027304532716916147 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.00603112168445939 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1823873.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.80603838486216 #Duplication Level Percentage of deduplicated Percentage of total 1 90.79064816142422 69.73270007672863 2 6.5764219206854895 10.102178289504373 3 1.2630803457355273 2.9103659255318366 4 0.4514382169117848 1.386927240660811 5 0.23352348568318795 0.8968006902574873 6 0.14308931375714112 0.659407399493674 7 0.09246700171022001 0.4971416857881792 8 0.07153330630734041 0.43953518960301524 9 0.0545130015600265 0.3768234921264102 >10 0.27833918070875624 3.8264780069853392 >50 0.021796632573819873 1.1694868579476125 >100 0.018179869613023574 2.7850733854251106 >500 0.002768756712057063 1.4952529946412116 >1k 0.002058819093580889 3.128787719076384 >5k 1.4198752369523374E-4 0.5930410462299539 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5465 0.2996370909597324 No Hit CTGTAGGACGTGGAATATGGCAAGA 5411 0.29667635849645235 No Hit GTCCTACAGTGGACATTTCTAAATT 4979 0.2729904987902118 No Hit CTTTAGGACGTGAAATATGGCGAGG 4118 0.2257832645145797 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3921 0.21498207386150245 No Hit TATCAACGCAGAGTACTTTTTTTTT 3668 0.20111049398724581 No Hit GGTATCAACGCAGAGTACTTTTTTT 3498 0.1917896695658086 No Hit TTGTAGAACAGTGTATATCAATGAG 2424 0.1329039905739051 No Hit CTGAAGGACCTGGAATATGGCGAGA 2375 0.13021740000537319 No Hit GTCCTACAGTGTGCATTTCTCATTT 2318 0.12709218240524423 No Hit GATATACACTGTTCTACAAATCCCG 2251 0.12341868101561897 No Hit CTGTAGGACCTGGAATATGGCGAGA 2123 0.11640064851006621 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1905 0.10444806189904669 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.4828378949630814E-5 0.0 5 0.0 0.0 0.0 5.4828378949630814E-5 0.0 6 0.0 0.0 0.0 5.4828378949630814E-5 0.0 7 0.0 0.0 0.0 1.0965675789926163E-4 0.0 8 0.0 0.0 0.0 1.0965675789926163E-4 0.0 9 0.0 0.0 0.0 1.0965675789926163E-4 0.0 10 0.0 0.0 0.0 1.0965675789926163E-4 0.0 11 0.0 0.0 0.0 1.0965675789926163E-4 0.0 12 0.0 0.0 0.0 1.0965675789926163E-4 0.0 13 0.0 0.0 0.0 1.6448513684889243E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 820 0.0 13.322882 4 CTAGGAC 195 0.0 12.179224 3 TCCAACG 305 0.0 12.144614 18 GATATAC 1095 0.0 12.100243 1 TAGGACA 765 0.0 11.92131 4 GGTATCA 1195 0.0 11.805576 1 CCAACGA 210 0.0 11.760036 19 TGTAGGA 2780 0.0 11.686797 2 CTGTAGG 2755 0.0 11.210289 1 GTAGGAC 2830 0.0 11.111067 3 AGGACGA 120 1.2758937E-7 11.083094 5 AGGACCG 60 0.0058772676 11.083094 5 CACCTTT 760 0.0 10.872379 14 CCTACAC 210 0.0 10.856908 3 GTCGAGG 140 8.372808E-9 10.855419 19 TGTCGAG 140 8.383722E-9 10.8545265 18 GTCTTAG 150 2.0172592E-9 10.803165 1 ACCTTTT 800 0.0 10.684925 15 ACTGTTC 1130 0.0 10.674419 8 GACGTGG 1490 0.0 10.645085 7 >>END_MODULE