FastQCFastQC Report
Thu 26 May 2016
SRR1512209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073613
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63920.2079637221732209No Hit
GTCCTACAGTGGACATTTCTAAATT53300.1734115518121507No Hit
CTGTAGGACGTGGAATATGGCAAGA46470.15119014657993704No Hit
GTCCTAAAGTGTGTATTTCTCATTT41800.13599630142116134No Hit
TATCAACGCAGAGTACTTTTTTTTT38510.1252922863093044No Hit
CTTTAGGACGTGAAATATGGCGAGG38010.12366553629230485No Hit
GGTATCAACGCAGAGTACTTTTTTT37350.12151822626986547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2650.012.80238318
TAGGACC8950.012.6352934
CGGTAGG751.069156E-412.2413951
GCGTAGA956.1687624E-611.81187251
TGTAGGA27800.011.1919222
GTCCTAA14450.011.1542111
GCCTTAG2500.011.0172561
GTAGGAC28750.010.9480243
CCAACGA2000.010.83757419
CTGTAGG28550.010.719261
TTAGTAC1855.456968E-1210.6973123
GATATAC7500.010.4731931
ACCGTGC1002.6243377E-510.36586958
AGGACGT29700.010.2805345
GCCGGTT1752.728484E-1010.23124611
GGTATCA20600.010.2011621
GTCCTAC26650.010.1820231
GGACGTG29750.010.0731966
TAGGACG31400.09.9940554
TCCTACA28950.09.9016472
Produced by FastQC (version 0.11.5)