FastQCFastQC Report
Thu 26 May 2016
SRR1512209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073613
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85930.2795732579215406No Hit
GTCCTACAGTGGACATTTCTAAATT62620.2037341721290221No Hit
CTGTAGGACGTGGAATATGGCAAGA55420.18030897188422876No Hit
TATCAACGCAGAGTACTTTTTTTTT51940.16898679176591197No Hit
GGTATCAACGCAGAGTACTTTTTTT51280.1668394817434726No Hit
GTCCTAAAGTGTGTATTTCTCATTT48820.15883587165983487No Hit
CTTTAGGACGTGAAATATGGCGAGG44230.1439023065037791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9150.012.7711054
TCCAACG1950.011.68849418
GGCGAGG11950.011.20664319
TGTAGGA29650.010.5738632
GTCCTAC27300.010.4844231
CTGTAGG29000.010.4291081
AGGACGT29600.010.3991475
GTAGGAC28250.010.358023
TTAGGAC18750.010.2858273
GGTATCA22450.010.24204351
GGACGTG29500.010.2051446
ACTAGAC1606.175469E-910.0942343
TACTCCG856.5939414E-410.0593065
TAGGACG31950.010.050564
AAGACCG1054.105596E-59.9528585
CTAATAC2201.8189894E-129.9322953
AGGACCT19150.09.8229265
TGGCGAG28200.09.69896218
GACGTGG14950.09.6557127
TCCTACA31800.09.6498342
Produced by FastQC (version 0.11.5)