FastQCFastQC Report
Thu 26 May 2016
SRR1512208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196371
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65523.3365415463586783No Hit
TATCAACGCAGAGTACTTTTTTTTT43072.193297380977843No Hit
GGTATCAACGCAGAGTACTTTTTTT41912.134225522098477No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT28431.4477697827072227No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA28351.4436958614051971No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23201.181437177587322No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21831.1116712752901396No Hit
GTACATAAGCAGTGGTATCAACGCA21181.0785706647111843No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14880.7577493621766961No Hit
GTACTGGTTCACTATCGGTCAGTCA12270.6248376796981224No Hit
GTGGTATCAACGCAGAGTACATGGG9780.4980368791725866No Hit
GCTTATGTACTCTGCGTTGATACCA9150.46595474891913774No Hit
ATACAGGGTGACAGCCCCGTACACA8630.43947426045597365No Hit
GGATACCACGTGTCCCGCCCTACTC8270.42114161459686No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7510.38243936222762015No Hit
GGTATCAACGCAGAGTACATGGGGG7270.37021759832154444No Hit
GTGGTATCAACGCAGAGTACTTTTT7170.36512519669401283No Hit
ATCAACGCAGAGTACTTTTTTTTTT6800.34628331067214607No Hit
GNATCAACGCAGAGTACTTTTTTTT6720.34220938937012085No Hit
GAGTACATAAGCAGTGGTATCAACG6610.33660774757983614No Hit
GTACATGGGGGTTAAGCGACTAAGC6370.32438598367376037No Hit
ATCATTAACTGAATCCATAGGTTAA6370.32438598367376037No Hit
CTTATGTACTCTGCGTTGATACCAC6290.32031206237173515No Hit
GGTATCAACGCAGAGAAAAAAAAAA6100.31063649927942516No Hit
GGTATCAACGCAGAAAAAAAAAAAA5800.2953592943968305No Hit
GTATCAACGCAGAGAAAAAAAAAAA5090.2592032428413564No Hit
GTATCAACGCAGAGTACATGGGGGT5000.25462008137657804No Hit
AGCGTACACGGTGGATGCCCTGGCA4950.2520738805628122No Hit
GTTAATGATAGTGTGTCGAAACACA4950.2520738805628122No Hit
GTGGATGCCCTGGCAGTCAGAGGCG4830.24596299860977436No Hit
GGTTAATGAGGCGAACCGGGGGAAC4820.2454537584470212No Hit
TATGTACTCTGCGTTGATACCACTG4680.238324396168477No Hit
ACTTAGATGTTTCAGTTCCCCCGGT4580.23323199454094545No Hit
TCTAAGTACCCCGAGGAAAAGAAAT4370.22253795112312916No Hit
GTATTTAGCCTTGGAGGATGGTCCC4330.22050099047211655No Hit
TNTCAACGCAGAGTACTTTTTTTTT4210.2143901085190787No Hit
GNTATCAACGCAGAGTACTTTTTTT4200.21388086835632553No Hit
CATAAGCAGTGGTATCAACGCAGAG4130.21031618721705342No Hit
GTTCACTATCGGTCAGTCAGGAGTA4110.20929770689154711No Hit
GCCCAGAGCCTGAATCAGTGTGTGT3940.20064062412474348No Hit
CCCATATTCAGACAGGATACCACGT3930.20013138396199034No Hit
TATTCAGACAGGATACCACGTGTCC3900.19860366347373085No Hit
GCCTTGGAGGATGGTCCCCCCATAT3890.19809442331097768No Hit
ACCCTGTATCGCGCGCCTTTCCAGA3760.19147430119518666No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA3750.1909650610324335No Hit
TNTTTTTTTTTTTTTTTTTTTTTTT3740.19045582086968035No Hit
GGTATCAACGCAGAGTACATGGGGA3710.18892810038142088No Hit
GTTATAACCGGCGATTTCCGAATGG3670.18689113973040827No Hit
GCGTTGATACCACTGCTTATGTACT3630.18485417907939564No Hit
GTACTTAGATGTTTCAGTTCCCCCG3600.18332645859113617No Hit
ACGCAGAGTACATGGGGGTTAAGCG3560.18128949794012356No Hit
GTCAGGAGTATTTAGCCTTGGAGGA3430.17466937582433253No Hit
GTGCTAATCTGCGATAAGCGTCGGT3400.17314165533607304No Hit
CTATCGGTCAGTCAGGAGTATTTAG3330.16957697419680096No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG3270.16652153322028201No Hit
ACGCAGAGTACATAAGCAGTGGTAT3170.16142913159275046No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3050.15531824963971258No Hit
CGGTACTGGTTCACTATCGGTCAGT3010.15328128898869994No Hit
GTATCAACGCAGAGTACATAAGCAG2990.15226280866319364No Hit
TATCAACGCAGAGTACATGGGGGTT2910.14818888736116842No Hit
TCACGGTACTGGTTCACTATCGGTC2870.14615192671015578No Hit
CAACGCAGAGTACTTTTTTTTTTTT2820.14360572589638998No Hit
TATCAACGCAGAGAAAAAAAAAAAA2810.14309648573363684No Hit
GGTATCAACGCAGAGTACATAAGCA2800.1425872455708837No Hit
CCCATGTACTCTGCGTTGATACCAC2770.1410595250826242No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2750.1400410447571179No Hit
TCAACGCAGAGTACTTTTTTTTTTT2690.13698560378059896No Hit
TCATTAACCTATGGATTCAGTTAAT2680.13647636361784582No Hit
GATATGAACCGTTATAACCGGCGAT2670.13596712345509265No Hit
AACGCAGAGTACTTTTTTTTTTTTT2640.13443940296683318No Hit
CATCTAAGTACCCCGAGGAAAAGAA2630.13393016280408004No Hit
CTGCTTATGTACTCTGCGTTGATAC2630.13393016280408004No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA2570.1308747218275611No Hit
CACACACACTGATTCAGGCTCTGGG2570.1308747218275611No Hit
TATCAACGCAGAGTACATAAGCAGT2570.1308747218275611No Hit
GTATCAACGCAGAAAAAAAAAAAAA2490.12680080052553586No Hit
GGGTACTTAGATGTTTCAGTTCCCC2470.12578232020002952No Hit
GGTGATATGAACCGTTATAACCGGC2450.12476383987452323No Hit
GCCTGAATCAGTGTGTGTGTTAGTG2440.12425459971177008No Hit
ACTCTGCGTTGATACCACTGCTTAT2430.1237453595490169No Hit
TCGGTAAGGTGATATGAACCGTTAT2380.12119915873525114No Hit
GNACTTTTTTTTTTTTTTTTTTTTT2340.1191621980842385No Hit
GGATCAACGCAGAGTACTTTTTTTT2320.1181437177587322No Hit
GAGTACATGGGGGTTAAGCGACTAA2320.1181437177587322No Hit
GTCCCGCCCTACTCATCGAGCTCAC2320.1181437177587322No Hit
GGTTCACTATCGGTCAGTCAGGAGT2260.11508827678221327No Hit
CCCCAGTAGCGGCGAGCGAACGGGG2230.11356055629395378No Hit
GGGTGACAGCCCCGTACACAAAAAT2200.11203283580569433No Hit
AGCTCACAGCATGTGCATTTTTGTG2200.11203283580569433No Hit
GTATCAACGCAGAGTACATGGGGGG2200.11203283580569433No Hit
GGGTTTCCCCATTCGGAAATCGCCG2190.11152359564294116No Hit
CACTATCATTAACTGAATCCATAGG2130.10846815466642222No Hit
ANGCAGAGTACTTTTTTTTTTTTTT2110.10744967434091592No Hit
GGAGTATTTAGCCTTGGAGGATGGT2110.10744967434091592No Hit
GTATCAACGCAGAGTACATGGGGAA2070.10541271368990329No Hit
CCTATGGATTCAGTTAATGATAGTG2060.10490347352715014No Hit
CCGTTATAACCGGCGATTTCCGAAT2050.10439423336439699No Hit
AAGCAGTGGTATCAACGCAGAGTAC2030.10337575303889068No Hit
GGTATCAACGCAGAGTACATGGGAA2020.1028665128761375No Hit
GCGTACACGGTGGATGCCCTGGCAG1980.1008295522251249No Hit
TTTGTGTACGGGGCTGTCACCCTGT1970.10032031206237174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGG402.9382412E-416.47794219
TGGGAAC400.005576541614.123956
TACTGGT2700.012.5642192
GAATCAA759.851874E-512.335781
GCGCGCG550.003262012511.98395811
CCGAATG550.003262012511.98395818
GTACTGG2850.011.90294551
ACTGGTT2950.011.8098553
ACAGGGT803.1065414E-511.76995853
ACTAAGC1701.8189894E-1211.63148819
GGTTCAC3300.011.127966
GCGACTA1701.2732926E-1111.07760716
TGGTTCA3250.011.0094385
TAGTGTG1659.094947E-1110.8426289
GAAACAC1855.456968E-1210.68839518
AGCGACT1804.0017767E-1110.46218515
CGACTAA1901.0913936E-1110.40712117
GACTAAG1901.0913936E-1110.40712118
CTGGTTC3450.010.3712094
GTCAGTC3450.010.37120918
Produced by FastQC (version 0.11.5)