FastQCFastQC Report
Thu 26 May 2016
SRR1512208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196371
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87784.470110148647204No Hit
TATCAACGCAGAGTACTTTTTTTTT60043.0574779371699488No Hit
GGTATCAACGCAGAGTACTTTTTTT59203.0147017634986835No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT35761.8210428220052859No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA34391.7512769197081037No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32711.6657245723655734No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27721.4116137311517485No Hit
GTACATAAGCAGTGGTATCAACGCA24011.2226856307703275No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19540.9950552780196669No Hit
GTACTGGTTCACTATCGGTCAGTCA13660.6956220623208111No Hit
GTGGTATCAACGCAGAGTACATGGG11840.6029403526997368No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11660.5937740297701799No Hit
GCTTATGTACTCTGCGTTGATACCA11240.5723859429345474No Hit
GTGGTATCAACGCAGAGTACTTTTT11100.5652565806560031No Hit
ATCAACGCAGAGTACTTTTTTTTTT9740.495999918521574No Hit
ATACAGGGTGACAGCCCCGTACACA9490.483268914452745No Hit
GGATACCACGTGTCCCGCCCTACTC9420.47970423331347295No Hit
GGTATCAACGCAGAGTACATGGGGG8090.41197529166730323No Hit
ATCATTAACTGAATCCATAGGTTAA7830.39873504743572113No Hit
CTTATGTACTCTGCGTTGATACCAC7800.3972073269474617No Hit
GAGTACATAAGCAGTGGTATCAACG7590.3865132835296454No Hit
GTACATGGGGGTTAAGCGACTAAGC6650.33864470823084875No Hit
AGCGTACACGGTGGATGCCCTGGCA6270.3192935820462288No Hit
GTATCAACGCAGAGTACATGGGGGT6220.31674738123246304No Hit
GGTATCAACGCAGAAAAAAAAAAAA6150.31318270009319094No Hit
GGTATCAACGCAGAGAAAAAAAAAA6100.31063649927942516No Hit
GTGGATGCCCTGGCAGTCAGAGGCG6060.30859953862841255No Hit
ACTTAGATGTTTCAGTTCCCCCGGT6010.3060533378146468No Hit
GGTTAATGAGGCGAACCGGGGGAAC6000.3055440976518936No Hit
GTTAATGATAGTGTGTCGAAACACA5600.2851744911417674No Hit
TATGTACTCTGCGTTGATACCACTG5580.2841560108162611No Hit
TATTCAGACAGGATACCACGTGTCC5470.27855436902597636No Hit
GTATCAACGCAGAGAAAAAAAAAAA5470.27855436902597636No Hit
ACCCTGTATCGCGCGCCTTTCCAGA5280.26887880593366636No Hit
GTATTTAGCCTTGGAGGATGGTCCC5110.26022172316686276No Hit
GTTCACTATCGGTCAGTCAGGAGTA5000.25462008137657804No Hit
TCTAAGTACCCCGAGGAAAAGAAAT4890.2490184395862933No Hit
CATAAGCAGTGGTATCAACGCAGAG4750.24188907730774914No Hit
CCCATATTCAGACAGGATACCACGT4720.2403613568194896No Hit
GCCTTGGAGGATGGTCCCCCCATAT4700.23934287649398334No Hit
GTACTTAGATGTTTCAGTTCCCCCG4660.23730591584297073No Hit
GCCCAGAGCCTGAATCAGTGTGTGT4430.22559339209964813No Hit
CAACGCAGAGTACTTTTTTTTTTTT4350.22151947079762285No Hit
GCGTTGATACCACTGCTTATGTACT4320.2199917503093634No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT4250.2164270691700913No Hit
CTATCGGTCAGTCAGGAGTATTTAG4220.21489934868183183No Hit
GTTATAACCGGCGATTTCCGAATGG4070.2072607462405345No Hit
ACGCAGAGTACATGGGGGTTAAGCG4030.20522378558952187No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG3980.2026775847757561No Hit
AACGCAGAGTACTTTTTTTTTTTTT3920.19962214379923715No Hit
GGTATCAACGCAGAGTACATGGGGA3890.19809442331097768No Hit
ACGCAGAGTACATAAGCAGTGGTAT3860.19656670282271824No Hit
GTCAGGAGTATTTAGCCTTGGAGGA3790.19300202168344613No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3760.19147430119518666No Hit
TCATTAACCTATGGATTCAGTTAAT3640.18536341924214877No Hit
TATCAACGCAGAGTACATGGGGGTT3630.18485417907939564No Hit
TATCAACGCAGAGAAAAAAAAAAAA3580.18230797826562986No Hit
CCCATGTACTCTGCGTTGATACCAC3490.17772481680085145No Hit
GGTTCACTATCGGTCAGTCAGGAGT3470.17670633647534514No Hit
TCACGGTACTGGTTCACTATCGGTC3470.17670633647534514No Hit
TCAACGCAGAGTACTTTTTTTTTTT3460.17619709631259198No Hit
GTATCAACGCAGAGTACATAAGCAG3430.17466937582433253No Hit
TATCAACGCAGAGTACATAAGCAGT3430.17466937582433253No Hit
CATCTAAGTACCCCGAGGAAAAGAA3370.1716139348478136No Hit
CGGTACTGGTTCACTATCGGTCAGT3320.16906773403404782No Hit
ACTCTGCGTTGATACCACTGCTTAT3170.16142913159275046No Hit
GTGCTAATCTGCGATAAGCGTCGGT3030.15429976931420628No Hit
CACTATCATTAACTGAATCCATAGG3020.15379052915145314No Hit
GGGTACTTAGATGTTTCAGTTCCCC2930.14920736768667472No Hit
GGTATCAACGCAGAGTACATAAGCA2910.14818888736116842No Hit
GAGTACATGGGGGTTAAGCGACTAA2900.14767964719841525No Hit
CTGCTTATGTACTCTGCGTTGATAC2870.14615192671015578No Hit
GATATGAACCGTTATAACCGGCGAT2870.14615192671015578No Hit
CACACACACTGATTCAGGCTCTGGG2860.14564268654740262No Hit
GTATCAACGCAGAAAAAAAAAAAAA2850.14513344638464948No Hit
CCGTTATAACCGGCGATTTCCGAAT2750.1400410447571179No Hit
GCCTGAATCAGTGTGTGTGTTAGTG2720.13851332426885843No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA2690.13698560378059896No Hit
CCCCAGTAGCGGCGAGCGAACGGGG2650.13494864312958635No Hit
GAATCAGTGTGTGTGTTAGTGGAAG2590.1318932021530674No Hit
GCGTACACGGTGGATGCCCTGGCAG2590.1318932021530674No Hit
TCGGTAAGGTGATATGAACCGTTAT2580.13138396199031424No Hit
AGCTCACAGCATGTGCATTTTTGTG2520.12832852101379533No Hit
GGAGTATTTAGCCTTGGAGGATGGT2500.12731004068828902No Hit
GGTGATATGAACCGTTATAACCGGC2500.12731004068828902No Hit
GGTATCAACGCAGAGTACATGGGAA2460.12527308003727639No Hit
GTGGTATCAACGCAGAGTACATAAG2440.12425459971177008No Hit
GTCCCGCCCTACTCATCGAGCTCAC2430.1237453595490169No Hit
CCTATGGATTCAGTTAATGATAGTG2430.1237453595490169No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG2370.12068991857249797No Hit
GGGTTTCCCCATTCGGAAATCGCCG2320.1181437177587322No Hit
GGTATCAACGCAGAGTACATGGGCA2210.11254207596844748No Hit
ATCCATAGGTTAATGAGGCGAACCG2190.11152359564294116No Hit
AAGCAGTGGTATCAACGCAGAGTAC2190.11152359564294116No Hit
ATGATAGTGTGTCGAAACACACTGG2150.10948663499192855No Hit
GTATCAACGCAGAGTACATGGGGAG2150.10948663499192855No Hit
GTATCAACGCAGAGTACATGGGGAA2130.10846815466642222No Hit
GGGTGACAGCCCCGTACACAAAAAT2110.10744967434091592No Hit
GCCCTACTCATCGAGCTCACAGCAT2100.10694043417816276No Hit
TTCCAGACGCTTCCACTAACACACA2090.10643119401540961No Hit
TAAGTACCCCGAGGAAAAGAAATCA2080.10592195385265646No Hit
GTGGTATCCTGTCTGAATATGGGGG2010.10235727271338435No Hit
CATGTACTCTGCGTTGATACCACTG2010.10235727271338435No Hit
CTATCATTAACTGAATCCATAGGTT2010.10235727271338435No Hit
GTCCCCCCATATTCAGACAGGATAC1970.10032031206237174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATGT400.00508204114.3229841
GACTAAG1501.8189894E-1212.66021618
AGAGTAA604.0813087E-412.66021511
TGAATCC1600.012.462399510
AATCCAT1700.012.28785612
GAATCCA1700.012.28785611
CCGAATG853.9162533E-612.28785618
CTTTCCA1405.0931703E-1112.20806517
TCATGTA550.003040416212.0970732
CAGAGGC1501.2732926E-1112.02720518
ACTGAAT1750.011.9367748
ACAGGGT1850.011.8168333
TAACTGA1850.011.8047966
ACTAAGC1552.5465852E-1111.63923119
CGAATGG907.4099607E-611.60519719
GACAGCC2200.011.22155410
CAGGGTG1950.011.210844
TTAACTG1950.011.210845
ATACAGG2300.011.2092911
GGTGACA1950.011.1994217
Produced by FastQC (version 0.11.5)