##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512208_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 196371 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.804894816444385 31.0 31.0 34.0 28.0 34.0 2 30.861293164469295 31.0 31.0 34.0 27.0 34.0 3 30.856964623085894 31.0 31.0 34.0 27.0 34.0 4 34.58780064266109 37.0 35.0 37.0 32.0 37.0 5 34.21139577636209 37.0 35.0 37.0 30.0 37.0 6 34.2478166328022 37.0 35.0 37.0 30.0 37.0 7 34.08463062264795 37.0 35.0 37.0 30.0 37.0 8 34.01176854016123 37.0 35.0 37.0 30.0 37.0 9 35.37436281324636 39.0 35.0 39.0 30.0 39.0 10 35.26146936156561 39.0 35.0 39.0 29.0 39.0 11 35.22240045627919 39.0 35.0 39.0 29.0 39.0 12 35.037250917905396 39.0 35.0 39.0 28.0 39.0 13 34.90241940001324 38.0 35.0 39.0 27.0 39.0 14 35.696126210081935 39.0 35.0 41.0 27.0 41.0 15 35.491987106039076 39.0 35.0 41.0 27.0 41.0 16 35.35752733346573 39.0 34.0 41.0 27.0 41.0 17 35.62820375717392 39.0 36.0 40.0 27.0 41.0 18 35.588029800734326 39.0 35.0 41.0 27.0 41.0 19 35.70568464793681 39.0 35.0 41.0 27.0 41.0 20 35.71296678226418 39.0 35.0 41.0 27.0 41.0 21 35.64172917589664 39.0 35.0 41.0 27.0 41.0 22 35.56195161199974 39.0 35.0 41.0 27.0 41.0 23 35.340340477972816 39.0 35.0 41.0 27.0 41.0 24 35.16301286849891 39.0 35.0 41.0 26.0 41.0 25 34.824057523768786 38.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.8091248793242443 1101 2 -0.8909669153547419 1101 3 -0.30405626709140776 1101 4 -0.6068494009710577 1101 5 -1.12942565131533 1101 6 -0.716846744182277 1101 7 -0.7956011762938004 1101 8 -0.49409551819166353 1101 9 -0.6293475637901338 1101 10 -0.8459698071702846 1101 11 -0.9902873078928991 1101 12 -0.9962738433688116 1101 13 -1.9097550683710267 1101 14 -1.7601088289340652 1101 15 -2.101236445651729 1101 16 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0.9838898932258608 1103 4 1.1002370870311893 1103 5 0.948449604062624 1103 6 1.0545349006520794 1103 7 1.170668903424179 1103 8 1.4369223883099593 1103 9 1.6934101175782956 1103 10 1.7332073750594148 1103 11 0.8227960554849005 1103 12 1.3050120597678259 1103 13 1.2824493942336304 1103 14 1.4058167409093016 1103 15 1.6051690344588394 1103 16 0.863817646904991 1103 17 1.1256498180627048 1103 18 1.789784940586074 1103 19 2.0941552825587237 1103 20 1.7900034303227983 1103 21 2.2034438112872863 1103 22 1.9600878978737057 1103 23 1.9753501786778074 1103 24 1.5195106044554478 1103 25 1.248604551203428 1104 1 0.046670268923733715 1104 2 0.6450842975832636 1104 3 0.520741576574359 1104 4 0.035670814662367434 1104 5 0.8347477538958046 1104 6 0.3189798506065813 1104 7 -0.05851671986972207 1104 8 -0.21590594047378175 1104 9 -0.07892078930675694 1104 10 -0.4899500407731594 1104 11 0.1410020002832013 1104 12 0.3051637127857205 1104 13 0.2879089028778523 1104 14 0.6075910812186791 1104 15 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0.2429722431597341 2314 19 0.375601090647379 2314 20 0.36674725508589034 2314 21 -0.15549668894573898 2314 22 0.2517351455563741 2314 23 0.03694719592186857 2314 24 0.5879689960457313 2314 25 -0.09925418216097626 2315 1 -0.7777603728982108 2315 2 -0.6325036941351634 2315 3 -0.6165255041709798 2315 4 -0.6348778395819394 2315 5 -1.0295897202242728 2315 6 -0.7137841245487024 2315 7 -0.8782372167641341 2315 8 -0.7529142220472806 2315 9 -1.1397385946897742 2315 10 -1.1287699165495582 2315 11 -1.2868965504414405 2315 12 -1.4080868048126192 2315 13 -1.445631469792957 2315 14 -1.741787800768904 2315 15 -1.4931970691135845 2315 16 -1.9647616939978079 2315 17 -1.6418107191845976 2315 18 -1.8531394570876998 2315 19 -1.7357810231737574 2315 20 -1.8753524267804949 2315 21 -1.5165783432405462 2315 22 -1.648441595341474 2315 23 -1.4908364732191686 2315 24 -1.227124676630126 2315 25 -1.5140650694002886 2316 1 -0.059472951723304135 2316 2 -0.45719669114810557 2316 3 -0.46126496650088455 2316 4 -0.08260435800212207 2316 5 -0.23499961069888542 2316 6 -0.2283211308547095 2316 7 -0.46077953339207767 2316 8 -0.34235995719497936 2316 9 -0.6795660092267823 2316 10 -1.1625236503696712 2316 11 -1.458552707095933 2316 12 -1.3380569342517177 2316 13 -1.0817748484852885 2316 14 -1.2738156799590783 2316 15 -1.3097918251706702 2316 16 -1.333829068707395 2316 17 -1.078949783240354 2316 18 -0.9131129054780089 2316 19 -0.8281474353874998 2316 20 -1.0543102761001109 2316 21 -0.8499669924274045 2316 22 -1.3053616086172752 2316 23 -0.671486987656607 2316 24 -0.6239716729792804 2316 25 -0.7580079834394482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3317.0 3 1.0 4 51.0 5 517.0 6 701.0 7 541.0 8 439.0 9 386.0 10 353.0 11 315.0 12 322.0 13 331.0 14 278.0 15 274.0 16 283.0 17 319.0 18 305.0 19 441.0 20 497.0 21 649.0 22 793.0 23 981.0 24 1176.0 25 1416.0 26 1730.0 27 1950.0 28 2329.0 29 2953.0 30 3765.0 31 4803.0 32 6190.0 33 8082.0 34 10768.0 35 14400.0 36 23342.0 37 37968.0 38 63405.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.85165369152234 18.91233849270084 15.32413990649712 14.9118679092797 2 16.53095416329295 23.31912553279252 39.63263414658987 20.517286157324655 3 21.175733687764485 32.00268878805934 30.25905047079253 16.562527053383647 4 12.25180907567818 19.977491584806312 34.56060212556844 33.21009721394707 5 10.313174946004318 41.3871795916704 31.3826969314153 16.91694853090998 6 25.313310010133875 36.35109053780853 20.291183525062255 18.04441592699533 7 22.114772547881305 39.27107363103513 20.408818002658233 18.20533581842533 8 27.13791751327844 30.176553564426516 21.48076854525363 21.204760377041417 9 29.43357216696966 15.309796253010882 20.70825121835709 34.54838036166236 10 21.108513986281068 28.394722234953225 25.317383931435906 25.1793798473298 11 30.18673836768158 27.508644351762733 19.98971334871239 22.314903931843297 12 27.20870189590113 29.68157212623045 26.37864043061348 16.73108554725494 13 31.84227813679209 23.150567038921228 28.366714026001805 16.640440798284878 14 25.56538389069669 27.284578680151345 29.31848389018745 17.831553538964513 15 27.933350647498866 26.185638408930036 29.828233293103363 16.052777650467736 16 18.643791598555794 30.405202397502684 29.63115735011789 21.31984865382363 17 18.16408736524232 29.991190145184373 28.998171827815717 22.846550661757593 18 16.80288841020314 23.42097356534315 39.11422766090716 20.66191036354655 19 18.337229020578395 28.089178137301335 37.25091790539336 16.322674936726912 20 20.563627012135193 22.839930539641802 38.879467945877956 17.71697450234505 21 21.937047731080455 25.695749372361497 36.5797393708847 15.787463525673342 22 20.55955309083317 25.104012303242335 36.22174353646923 18.114691069455265 23 21.601967703988876 24.499034989891584 36.96116025278682 16.93783705333272 24 19.17391060798183 25.419741204149286 35.92434728142139 19.48200090644749 25 19.426153117504164 27.098834278031564 37.50833931727092 15.966673287193355 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7096.0 1 7096.0 2 4399.0 3 1702.0 4 1702.0 5 1702.0 6 2504.0 7 3306.0 8 3306.0 9 3306.0 10 2820.5 11 2335.0 12 2335.0 13 2335.0 14 1497.5 15 660.0 16 660.0 17 660.0 18 1670.0 19 2680.0 20 2680.0 21 2680.0 22 4726.5 23 6773.0 24 6773.0 25 6773.0 26 10068.0 27 13363.0 28 13363.0 29 13363.0 30 14104.5 31 14846.0 32 14846.0 33 14846.0 34 14274.5 35 13703.0 36 13703.0 37 13703.0 38 12151.0 39 10599.0 40 10599.0 41 10599.0 42 16500.5 43 22402.0 44 22402.0 45 22402.0 46 24643.0 47 26884.0 48 26884.0 49 26884.0 50 25764.0 51 24644.0 52 24644.0 53 24644.0 54 20948.5 55 17253.0 56 17253.0 57 17253.0 58 14042.0 59 10831.0 60 10831.0 61 10831.0 62 10131.0 63 9431.0 64 9431.0 65 9431.0 66 6780.5 67 4130.0 68 4130.0 69 4130.0 70 3292.0 71 2454.0 72 2454.0 73 2454.0 74 1705.0 75 956.0 76 956.0 77 956.0 78 597.5 79 239.0 80 239.0 81 239.0 82 148.0 83 57.0 84 57.0 85 57.0 86 38.0 87 19.0 88 19.0 89 19.0 90 12.0 91 5.0 92 5.0 93 5.0 94 4.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4425297014324926 2 0.0 3 0.0 4 0.0 5 0.0300451696024362 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006110881953037873 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 196371.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.69131389054392 #Duplication Level Percentage of deduplicated Percentage of total 1 78.62975540886022 20.98731482754582 2 11.027588048994543 5.8868162814264835 3 3.8081428625939635 3.049330094565898 4 1.6903880642576412 1.804747136797185 5 0.9463120540313656 1.2629156036278268 6 0.6162475674438127 0.9869074354156163 7 0.3892089899645133 0.7271949524115068 8 0.3033540657076354 0.6477534870220144 9 0.2289464646850078 0.5499793757734085 >10 1.598809478383638 9.054799333913866 >50 0.3624985690845957 7.049411572991938 >100 0.33197237379326133 18.325516496835075 >500 0.040065631319876366 7.227645629955544 >1k 0.020986759262792386 11.897377922401985 >5k 0.005723661617125195 10.542289849315836 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8778 4.470110148647204 No Hit TATCAACGCAGAGTACTTTTTTTTT 6004 3.0574779371699488 No Hit GGTATCAACGCAGAGTACTTTTTTT 5920 3.0147017634986835 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 3576 1.8210428220052859 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 3439 1.7512769197081037 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3271 1.6657245723655734 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2772 1.4116137311517485 No Hit GTACATAAGCAGTGGTATCAACGCA 2401 1.2226856307703275 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1954 0.9950552780196669 No Hit GTACTGGTTCACTATCGGTCAGTCA 1366 0.6956220623208111 No Hit GTGGTATCAACGCAGAGTACATGGG 1184 0.6029403526997368 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1166 0.5937740297701799 No Hit GCTTATGTACTCTGCGTTGATACCA 1124 0.5723859429345474 No Hit GTGGTATCAACGCAGAGTACTTTTT 1110 0.5652565806560031 No Hit ATCAACGCAGAGTACTTTTTTTTTT 974 0.495999918521574 No Hit ATACAGGGTGACAGCCCCGTACACA 949 0.483268914452745 No Hit GGATACCACGTGTCCCGCCCTACTC 942 0.47970423331347295 No Hit GGTATCAACGCAGAGTACATGGGGG 809 0.41197529166730323 No Hit ATCATTAACTGAATCCATAGGTTAA 783 0.39873504743572113 No Hit CTTATGTACTCTGCGTTGATACCAC 780 0.3972073269474617 No Hit GAGTACATAAGCAGTGGTATCAACG 759 0.3865132835296454 No Hit GTACATGGGGGTTAAGCGACTAAGC 665 0.33864470823084875 No Hit AGCGTACACGGTGGATGCCCTGGCA 627 0.3192935820462288 No Hit GTATCAACGCAGAGTACATGGGGGT 622 0.31674738123246304 No Hit GGTATCAACGCAGAAAAAAAAAAAA 615 0.31318270009319094 No Hit GGTATCAACGCAGAGAAAAAAAAAA 610 0.31063649927942516 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 606 0.30859953862841255 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 601 0.3060533378146468 No Hit GGTTAATGAGGCGAACCGGGGGAAC 600 0.3055440976518936 No Hit GTTAATGATAGTGTGTCGAAACACA 560 0.2851744911417674 No Hit TATGTACTCTGCGTTGATACCACTG 558 0.2841560108162611 No Hit TATTCAGACAGGATACCACGTGTCC 547 0.27855436902597636 No Hit GTATCAACGCAGAGAAAAAAAAAAA 547 0.27855436902597636 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 528 0.26887880593366636 No Hit GTATTTAGCCTTGGAGGATGGTCCC 511 0.26022172316686276 No Hit GTTCACTATCGGTCAGTCAGGAGTA 500 0.25462008137657804 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 489 0.2490184395862933 No Hit CATAAGCAGTGGTATCAACGCAGAG 475 0.24188907730774914 No Hit CCCATATTCAGACAGGATACCACGT 472 0.2403613568194896 No Hit GCCTTGGAGGATGGTCCCCCCATAT 470 0.23934287649398334 No Hit GTACTTAGATGTTTCAGTTCCCCCG 466 0.23730591584297073 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 443 0.22559339209964813 No Hit CAACGCAGAGTACTTTTTTTTTTTT 435 0.22151947079762285 No Hit GCGTTGATACCACTGCTTATGTACT 432 0.2199917503093634 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 425 0.2164270691700913 No Hit CTATCGGTCAGTCAGGAGTATTTAG 422 0.21489934868183183 No Hit GTTATAACCGGCGATTTCCGAATGG 407 0.2072607462405345 No Hit ACGCAGAGTACATGGGGGTTAAGCG 403 0.20522378558952187 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 398 0.2026775847757561 No Hit AACGCAGAGTACTTTTTTTTTTTTT 392 0.19962214379923715 No Hit GGTATCAACGCAGAGTACATGGGGA 389 0.19809442331097768 No Hit ACGCAGAGTACATAAGCAGTGGTAT 386 0.19656670282271824 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 379 0.19300202168344613 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 376 0.19147430119518666 No Hit TCATTAACCTATGGATTCAGTTAAT 364 0.18536341924214877 No Hit TATCAACGCAGAGTACATGGGGGTT 363 0.18485417907939564 No Hit TATCAACGCAGAGAAAAAAAAAAAA 358 0.18230797826562986 No Hit CCCATGTACTCTGCGTTGATACCAC 349 0.17772481680085145 No Hit GGTTCACTATCGGTCAGTCAGGAGT 347 0.17670633647534514 No Hit TCACGGTACTGGTTCACTATCGGTC 347 0.17670633647534514 No Hit TCAACGCAGAGTACTTTTTTTTTTT 346 0.17619709631259198 No Hit GTATCAACGCAGAGTACATAAGCAG 343 0.17466937582433253 No Hit TATCAACGCAGAGTACATAAGCAGT 343 0.17466937582433253 No Hit CATCTAAGTACCCCGAGGAAAAGAA 337 0.1716139348478136 No Hit CGGTACTGGTTCACTATCGGTCAGT 332 0.16906773403404782 No Hit ACTCTGCGTTGATACCACTGCTTAT 317 0.16142913159275046 No Hit GTGCTAATCTGCGATAAGCGTCGGT 303 0.15429976931420628 No Hit CACTATCATTAACTGAATCCATAGG 302 0.15379052915145314 No Hit GGGTACTTAGATGTTTCAGTTCCCC 293 0.14920736768667472 No Hit GGTATCAACGCAGAGTACATAAGCA 291 0.14818888736116842 No Hit GAGTACATGGGGGTTAAGCGACTAA 290 0.14767964719841525 No Hit CTGCTTATGTACTCTGCGTTGATAC 287 0.14615192671015578 No Hit GATATGAACCGTTATAACCGGCGAT 287 0.14615192671015578 No Hit CACACACACTGATTCAGGCTCTGGG 286 0.14564268654740262 No Hit GTATCAACGCAGAAAAAAAAAAAAA 285 0.14513344638464948 No Hit CCGTTATAACCGGCGATTTCCGAAT 275 0.1400410447571179 No Hit GCCTGAATCAGTGTGTGTGTTAGTG 272 0.13851332426885843 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 269 0.13698560378059896 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 265 0.13494864312958635 No Hit GAATCAGTGTGTGTGTTAGTGGAAG 259 0.1318932021530674 No Hit GCGTACACGGTGGATGCCCTGGCAG 259 0.1318932021530674 No Hit TCGGTAAGGTGATATGAACCGTTAT 258 0.13138396199031424 No Hit AGCTCACAGCATGTGCATTTTTGTG 252 0.12832852101379533 No Hit GGAGTATTTAGCCTTGGAGGATGGT 250 0.12731004068828902 No Hit GGTGATATGAACCGTTATAACCGGC 250 0.12731004068828902 No Hit GGTATCAACGCAGAGTACATGGGAA 246 0.12527308003727639 No Hit GTGGTATCAACGCAGAGTACATAAG 244 0.12425459971177008 No Hit GTCCCGCCCTACTCATCGAGCTCAC 243 0.1237453595490169 No Hit CCTATGGATTCAGTTAATGATAGTG 243 0.1237453595490169 No Hit GTGTTAGTGGAAGCGTCTGGAAAGG 237 0.12068991857249797 No Hit GGGTTTCCCCATTCGGAAATCGCCG 232 0.1181437177587322 No Hit GGTATCAACGCAGAGTACATGGGCA 221 0.11254207596844748 No Hit ATCCATAGGTTAATGAGGCGAACCG 219 0.11152359564294116 No Hit AAGCAGTGGTATCAACGCAGAGTAC 219 0.11152359564294116 No Hit ATGATAGTGTGTCGAAACACACTGG 215 0.10948663499192855 No Hit GTATCAACGCAGAGTACATGGGGAG 215 0.10948663499192855 No Hit GTATCAACGCAGAGTACATGGGGAA 213 0.10846815466642222 No Hit GGGTGACAGCCCCGTACACAAAAAT 211 0.10744967434091592 No Hit GCCCTACTCATCGAGCTCACAGCAT 210 0.10694043417816276 No Hit TTCCAGACGCTTCCACTAACACACA 209 0.10643119401540961 No Hit TAAGTACCCCGAGGAAAAGAAATCA 208 0.10592195385265646 No Hit GTGGTATCCTGTCTGAATATGGGGG 201 0.10235727271338435 No Hit CATGTACTCTGCGTTGATACCACTG 201 0.10235727271338435 No Hit CTATCATTAACTGAATCCATAGGTT 201 0.10235727271338435 No Hit GTCCCCCCATATTCAGACAGGATAC 197 0.10032031206237174 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCATGT 40 0.005082041 14.322984 1 GACTAAG 150 1.8189894E-12 12.660216 18 AGAGTAA 60 4.0813087E-4 12.660215 11 TGAATCC 160 0.0 12.4623995 10 AATCCAT 170 0.0 12.287856 12 GAATCCA 170 0.0 12.287856 11 CCGAATG 85 3.9162533E-6 12.287856 18 CTTTCCA 140 5.0931703E-11 12.208065 17 TCATGTA 55 0.0030404162 12.097073 2 CAGAGGC 150 1.2732926E-11 12.027205 18 ACTGAAT 175 0.0 11.936774 8 ACAGGGT 185 0.0 11.816833 3 TAACTGA 185 0.0 11.804796 6 ACTAAGC 155 2.5465852E-11 11.639231 19 CGAATGG 90 7.4099607E-6 11.605197 19 GACAGCC 220 0.0 11.221554 10 CAGGGTG 195 0.0 11.21084 4 TTAACTG 195 0.0 11.21084 5 ATACAGG 230 0.0 11.209291 1 GGTGACA 195 0.0 11.199421 7 >>END_MODULE