FastQCFastQC Report
Thu 26 May 2016
SRR1512207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244779
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52900.4249750357292339No Hit
GTCCTACAGTGGACATTTCTAAATT33320.2676780376275628No Hit
TATCAACGCAGAGTACTTTTTTTTT33150.26631233335395277No Hit
GGTATCAACGCAGAGTACTTTTTTT32380.2601264963499545No Hit
CTGTAGGACGTGGAATATGGCAAGA30940.2485581777970226No Hit
GTCCTAAAGTGTGTATTTCTCATTT29710.23867690569972663No Hit
CTTTAGGACGTGAAATATGGCGAGG26490.21280886004664282No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15330.12315439126142069No Hit
GTCCTACAGTGTGCATTTCTCATTT13840.11118439498095645No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13160.1057215778865164No Hit
CTGAAGGACCTGGAATATGGCGAGA12660.10170480061119283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9400.014.0536931
TAGGACC5300.013.1518654
GTCGAGG1052.239176E-812.56095319
CCGTGCC604.395065E-412.5594389
GTATTAG852.4708166E-512.0478531
GTCTAAT600.003354302111.9474551
CGGTAGG600.003354302111.9474551
TATGTCG1354.274625E-1011.86216816
TGTAGGA17350.011.8048142
GATATAC2200.011.6371321
GTATTAA1851.8189894E-1211.6245521
TAGGACT2200.011.5603924
GTCCTAC15650.011.2549291
CTGTAGG17950.011.2390711
GTATCAA19550.011.2097421
GGCGAGG9350.011.18394919
GACGTGA11650.011.1579567
TTAGGAC12950.011.1289233
AGGACGT21000.011.0343655
GCAATGC600.00625814410.9895098
Produced by FastQC (version 0.11.5)