FastQCFastQC Report
Thu 26 May 2016
SRR1512207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244779
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72950.5860478044697092No Hit
TATCAACGCAGAGTACTTTTTTTTT47230.37942478142706454No Hit
GGTATCAACGCAGAGTACTTTTTTT46850.3763720306978186No Hit
GTCCTACAGTGGACATTTCTAAATT39090.3140316473847968No Hit
CTGTAGGACGTGGAATATGGCAAGA35150.28237944245524704No Hit
GTCCTAAAGTGTGTATTTCTCATTT32860.26398260253426514No Hit
CTTTAGGACGTGAAATATGGCGAGG31030.24928119770658086No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21280.17095404083777122No Hit
GTCCTACAGTGTGCATTTCTCATTT16130.1295812349019384No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15780.1267694908092119No Hit
CTGAAGGACCTGGAATATGGCGAGA14430.11592419216583827No Hit
CTGTAGGACCTGGAATATGGCGAGA13760.1105417106169047No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12710.10210647833872519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTG400.00529033114.245127
GGTATCA12050.012.6677971
CGGTTTC853.956655E-612.28990713
GTATAAG802.7433845E-511.9255431
TAGGACC5800.011.63046554
ATTACAC907.448856E-611.6122663
GTCTTAT751.9929737E-411.4485211
GCCGGTT752.0797894E-411.39609511
CCGGTTT752.0797894E-411.39609512
CCCCGTA855.339249E-511.17264315
TCCAACG855.339249E-511.17264318
GGCGAGG9300.011.13238419
GATCTAG600.005691692711.13050651
GAAATGT4800.011.0795386
AATGTCC4950.010.9356488
TAGTACT1053.4596742E-610.8582224
AAATGTC5250.010.8534247
ATTTAGA4900.010.5139481
GATATAC2001.8189894E-1210.4944771
TGCCGGT1002.4064E-510.44642110
Produced by FastQC (version 0.11.5)