##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512207_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1244779 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35749960434744 31.0 31.0 34.0 28.0 34.0 2 31.546407836250452 33.0 31.0 34.0 28.0 34.0 3 31.633890031885176 33.0 31.0 34.0 28.0 34.0 4 35.236533553345616 37.0 35.0 37.0 32.0 37.0 5 34.91583405568378 37.0 35.0 37.0 32.0 37.0 6 34.87582293724428 37.0 35.0 37.0 32.0 37.0 7 34.73465008648122 37.0 35.0 37.0 32.0 37.0 8 34.65820037131089 37.0 35.0 37.0 32.0 37.0 9 36.22343082587351 39.0 37.0 39.0 32.0 39.0 10 36.055796249776066 39.0 35.0 39.0 31.0 39.0 11 36.04228140095551 39.0 37.0 39.0 31.0 39.0 12 35.90220673709952 39.0 35.0 39.0 30.0 39.0 13 35.884560231173566 39.0 35.0 39.0 30.0 39.0 14 36.97421871673606 40.0 37.0 41.0 31.0 41.0 15 36.91345612353679 40.0 37.0 41.0 31.0 41.0 16 36.835218942478946 40.0 36.0 41.0 31.0 41.0 17 36.80174472737731 40.0 36.0 41.0 31.0 41.0 18 36.75932113250625 40.0 36.0 41.0 30.0 41.0 19 36.78628334828913 40.0 37.0 41.0 30.0 41.0 20 36.70056371452282 40.0 36.0 41.0 30.0 41.0 21 36.59316392709067 40.0 36.0 41.0 30.0 41.0 22 36.68442269671966 40.0 37.0 41.0 30.0 41.0 23 36.584935157164445 40.0 37.0 41.0 30.0 41.0 24 36.4888683051369 40.0 36.0 41.0 30.0 41.0 25 36.098079257442485 39.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13270865800254938 1101 2 0.12067430303949678 1101 3 0.14890794621541303 1101 4 0.04965822591301361 1101 5 0.13051366191173486 1101 6 0.22002351613985383 1101 7 0.18353227366448976 1101 8 0.11871877433271294 1101 9 0.12551636484344897 1101 10 0.09049643384543771 1101 11 0.08846251136935734 1101 12 -0.07281338167032914 1101 13 0.022401924523592243 1101 14 -0.1654332888615926 1101 15 -0.19705445588565595 1101 16 -0.053380282400389945 1101 17 0.020652568275430383 1101 18 0.04592131267501287 1101 19 -0.01250104021453069 1101 20 -0.16869918520647076 1101 21 -0.23405820477513828 1101 22 -0.15885156540554135 1101 23 -0.14692770918018994 1101 24 -0.08114801916580205 1101 25 -0.18110746698211244 1102 1 0.35983189939830496 1102 2 0.442156100988786 1102 3 0.3950380233158022 1102 4 0.4208379810994032 1102 5 0.4711215229720622 1102 6 0.49140165248301315 1102 7 0.4691647088180275 1102 8 0.47165839227511697 1102 9 0.6507428969156237 1102 10 0.5297484143451356 1102 11 0.7471243824447882 1102 12 0.6973095550331649 1102 13 0.5797072526639084 1102 14 0.5730447070581803 1102 15 0.7080537761043786 1102 16 0.7125365763548785 1102 17 0.9646554297331846 1102 18 0.9582212755820407 1102 19 0.8231125172663241 1102 20 0.9970649962311526 1102 21 0.9133515798206773 1102 22 0.8447749348726603 1102 23 0.7351696333733955 1102 24 0.7941417696692099 1102 25 0.6613169560897205 1103 1 0.33910044834000175 1103 2 0.3252700690486918 1103 3 0.34451555827415703 1103 4 0.38519669585450345 1103 5 0.1874000818472794 1103 6 0.2787637930296114 1103 7 0.19802095292796196 1103 8 0.3104933684601292 1103 9 0.1709824476147901 1103 10 0.4480627084283171 1103 11 0.2931337810846557 1103 12 0.5021858954218459 1103 13 0.24750417581920203 1103 14 0.3843300793105442 1103 15 0.3379664120535395 1103 16 0.6214156166238496 1103 17 0.19744369289509933 1103 18 0.4590920506514493 1103 19 0.568058201278447 1103 20 0.3415082732745276 1103 21 0.5262737544978364 1103 22 0.6044314642529613 1103 23 0.7039299807919548 1103 24 0.6758007314888275 1103 25 0.7004047276696568 1104 1 0.1753355789057025 1104 2 0.1816948484544305 1104 3 0.3182142266649066 1104 4 0.15968212767896262 1104 5 0.3108235955109251 1104 6 0.2546084558170989 1104 7 0.15085915952013806 1104 8 0.12867573852426517 1104 9 0.3435134158012545 1104 10 0.349372766676737 1104 11 0.2855620936410048 1104 12 0.27760166286525845 1104 13 0.3969239681403707 1104 14 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-0.2574925692372929 2314 7 -0.4525033447150122 2314 8 -0.46520946907787675 2314 9 -0.3879023822902994 2314 10 -0.5432892935609956 2314 11 -0.5183701344799374 2314 12 -0.290270561265487 2314 13 -0.5006455562358099 2314 14 -0.5296019788264914 2314 15 -0.34699491281158146 2314 16 -0.19417684752102105 2314 17 -0.4643023401856894 2314 18 -0.44245123078268733 2314 19 -0.4902926606983513 2314 20 -0.5167956187450287 2314 21 -0.6736885253830422 2314 22 -0.4618352169892077 2314 23 -0.5752061083524609 2314 24 -0.4042476079249724 2314 25 -0.5540745364756887 2315 1 -0.1616798181471779 2315 2 -0.27922456506117044 2315 3 -0.24145210927747485 2315 4 -0.2642953830695731 2315 5 -0.08907351248893036 2315 6 -0.27302172093666144 2315 7 -0.2762639066614696 2315 8 -0.1436977594362645 2315 9 -0.25826882062986556 2315 10 -0.2976452026761436 2315 11 -0.24987685098105317 2315 12 -0.35478726889529355 2315 13 -0.13406857218961932 2315 14 -0.47859729109168114 2315 15 -0.29721911936277934 2315 16 -0.562116522627548 2315 17 -0.3479563494835958 2315 18 -0.36595001643152614 2315 19 -0.3579274148952578 2315 20 -0.4952839091034136 2315 21 -0.34796124780805826 2315 22 -0.3357908992315686 2315 23 -0.09671442459761437 2315 24 0.021766654035538124 2315 25 -0.06255754283103698 2316 1 -0.5373747392178707 2316 2 -0.40628359045581774 2316 3 -0.45521326233169646 2316 4 -0.45153228538026013 2316 5 -0.4361365415440659 2316 6 -0.3580022745857079 2316 7 -0.43654473484723866 2316 8 -0.37064525428870354 2316 9 -0.3996895481521676 2316 10 -0.6335580372677683 2316 11 -0.6954013305051916 2316 12 -0.8007035351959146 2316 13 -0.7084823219036451 2316 14 -1.0199894210390639 2316 15 -0.8292884398844009 2316 16 -0.7577182156039797 2316 17 -0.9387164730251172 2316 18 -0.6849531049696225 2316 19 -0.5778341868009989 2316 20 -0.4531453824504794 2316 21 -0.562571748837577 2316 22 -0.5353676551226627 2316 23 -0.3676821665331005 2316 24 -0.5565478048983437 2316 25 -0.5558113749060709 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10958.0 3 2.0 4 181.0 5 1958.0 6 2493.0 7 2053.0 8 1653.0 9 1580.0 10 1470.0 11 1425.0 12 1421.0 13 1345.0 14 1313.0 15 1330.0 16 1337.0 17 1477.0 18 1632.0 19 1861.0 20 2313.0 21 2914.0 22 3812.0 23 4992.0 24 6316.0 25 7645.0 26 8962.0 27 10378.0 28 12441.0 29 15652.0 30 20462.0 31 26467.0 32 35318.0 33 46238.0 34 60822.0 35 81983.0 36 119789.0 37 207063.0 38 535723.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.58410168465651 19.814465852920407 13.995359628770302 24.606072833652778 2 14.473733891718933 20.318867847224286 41.9949244002349 23.21247386082188 3 18.609729116574105 24.631360265557177 30.374628749360326 26.384281868508385 4 12.694140887659577 17.27985449625998 37.845995152553186 32.180009463527256 5 12.907858298924916 37.984805113608054 35.7482444875543 13.359092099912726 6 31.586329782234436 37.318270954121175 17.711095704538717 13.384303559105673 7 27.789270223871064 32.04633111580449 22.01764329250413 18.14675536782031 8 24.70510829633212 37.9603929693544 20.163820244396796 17.170678489916686 9 26.21846930258303 15.58340878179982 20.859767075119358 37.338354840497786 10 15.891656269908152 28.86520418483924 33.801823456211906 21.441316089040704 11 33.378615802483814 22.56360365976611 25.383059964861232 18.67472057288884 12 24.147418939426196 24.677472868677892 31.38774031374244 19.78736787815347 13 29.157705906028298 22.382929017922056 25.957860792960037 22.50150428308961 14 20.534247444727136 23.386480652388897 27.61405839912145 28.465213503762516 15 22.372324725915202 32.41820435595395 23.572377104690872 21.637093813439975 16 21.098925994092124 28.4427195510207 29.11769880436608 21.340655650521096 17 20.88202002122465 29.508290226618538 28.558563407641035 21.051126344515776 18 20.75677690578006 27.10432936288289 31.758087178527273 20.380806552809776 19 23.274653573043892 26.71446096053998 28.538318850173404 21.472566616242723 20 23.429138827052835 27.267651526897545 29.225830448617785 20.07737919743183 21 22.217678800815246 26.737838604282366 28.26726671963457 22.77721587526782 22 23.111090402392716 28.48770745650433 28.136239444913514 20.264962696189446 23 21.367728729356777 28.299722280019186 29.3501095375163 20.98243945310774 24 23.101691143568456 26.489682104212875 29.767854374149948 20.640772378068718 25 21.97047422017796 27.630184586337798 29.53718842268062 20.862152770803625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 658.0 1 658.0 2 652.5 3 647.0 4 647.0 5 647.0 6 1477.0 7 2307.0 8 2307.0 9 2307.0 10 2894.5 11 3482.0 12 3482.0 13 3482.0 14 4765.0 15 6048.0 16 6048.0 17 6048.0 18 10756.0 19 15464.0 20 15464.0 21 15464.0 22 24797.5 23 34131.0 24 34131.0 25 34131.0 26 50620.5 27 67110.0 28 67110.0 29 67110.0 30 85927.0 31 104744.0 32 104744.0 33 104744.0 34 116082.5 35 127421.0 36 127421.0 37 127421.0 38 133266.5 39 139112.0 40 139112.0 41 139112.0 42 146257.0 43 153402.0 44 153402.0 45 153402.0 46 156451.0 47 159500.0 48 159500.0 49 159500.0 50 152198.0 51 144896.0 52 144896.0 53 144896.0 54 127695.5 55 110495.0 56 110495.0 57 110495.0 58 94321.0 59 78147.0 60 78147.0 61 78147.0 62 64131.0 63 50115.0 64 50115.0 65 50115.0 66 38671.5 67 27228.0 68 27228.0 69 27228.0 70 19922.0 71 12616.0 72 12616.0 73 12616.0 74 8716.5 75 4817.0 76 4817.0 77 4817.0 78 3279.5 79 1742.0 80 1742.0 81 1742.0 82 1134.5 83 527.0 84 527.0 85 527.0 86 333.0 87 139.0 88 139.0 89 139.0 90 83.0 91 27.0 92 27.0 93 27.0 94 15.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45421717429358943 2 0.0 3 0.0 4 0.0 5 0.0331785802941727 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.008354896732673029 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1244779.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.89995224581398 #Duplication Level Percentage of deduplicated Percentage of total 1 83.14431144146475 57.28639087828006 2 11.657219766196786 16.063637704198346 3 2.7729725736594464 5.731730337122632 4 0.9753071416342294 2.687944619343989 5 0.4587915520851451 1.5805358014724686 6 0.2516610871081058 1.0403662130326874 7 0.1493117408774473 0.7201300271336829 8 0.10186797892707641 0.5614959106762519 9 0.08093715510191173 0.5018909509290381 >10 0.35266683544458355 4.333127232273055 >50 0.02721281326993359 1.3194882020059633 >100 0.022728101093083417 3.132485365422314 >500 0.002913844847353683 1.379488022157191 >1k 0.001981414496200504 3.075460052657698 >5k 1.1655379389414731E-4 0.5858286832947204 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7295 0.5860478044697092 No Hit TATCAACGCAGAGTACTTTTTTTTT 4723 0.37942478142706454 No Hit GGTATCAACGCAGAGTACTTTTTTT 4685 0.3763720306978186 No Hit GTCCTACAGTGGACATTTCTAAATT 3909 0.3140316473847968 No Hit CTGTAGGACGTGGAATATGGCAAGA 3515 0.28237944245524704 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3286 0.26398260253426514 No Hit CTTTAGGACGTGAAATATGGCGAGG 3103 0.24928119770658086 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2128 0.17095404083777122 No Hit GTCCTACAGTGTGCATTTCTCATTT 1613 0.1295812349019384 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1578 0.1267694908092119 No Hit CTGAAGGACCTGGAATATGGCGAGA 1443 0.11592419216583827 No Hit CTGTAGGACCTGGAATATGGCGAGA 1376 0.1105417106169047 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1271 0.10210647833872519 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 8.033554550647142E-5 3 0.0 0.0 0.0 0.0 8.033554550647142E-5 4 0.0 0.0 0.0 0.0 8.033554550647142E-5 5 0.0 0.0 0.0 0.0 8.033554550647142E-5 6 0.0 0.0 0.0 0.0 8.033554550647142E-5 7 0.0 0.0 0.0 0.0 8.033554550647142E-5 8 0.0 0.0 0.0 0.0 8.033554550647142E-5 9 0.0 0.0 0.0 0.0 8.033554550647142E-5 10 0.0 0.0 0.0 0.0 8.033554550647142E-5 11 0.0 0.0 0.0 0.0 8.033554550647142E-5 12 0.0 0.0 0.0 0.0 8.033554550647142E-5 13 0.0 0.0 0.0 0.0 8.033554550647142E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTTG 40 0.005290331 14.24512 7 GGTATCA 1205 0.0 12.667797 1 CGGTTTC 85 3.956655E-6 12.289907 13 GTATAAG 80 2.7433845E-5 11.925543 1 TAGGACC 580 0.0 11.6304655 4 ATTACAC 90 7.448856E-6 11.612266 3 GTCTTAT 75 1.9929737E-4 11.448521 1 GCCGGTT 75 2.0797894E-4 11.396095 11 CCGGTTT 75 2.0797894E-4 11.396095 12 CCCCGTA 85 5.339249E-5 11.172643 15 TCCAACG 85 5.339249E-5 11.172643 18 GGCGAGG 930 0.0 11.132384 19 GATCTAG 60 0.0056916927 11.1305065 1 GAAATGT 480 0.0 11.079538 6 AATGTCC 495 0.0 10.935648 8 TAGTACT 105 3.4596742E-6 10.858222 4 AAATGTC 525 0.0 10.853424 7 ATTTAGA 490 0.0 10.513948 1 GATATAC 200 1.8189894E-12 10.494477 1 TGCCGGT 100 2.4064E-5 10.446421 10 >>END_MODULE