FastQCFastQC Report
Thu 26 May 2016
SRR1512206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2723002
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79210.29089218443467907No Hit
CTGTAGGACGTGGAATATGGCAAGA71490.26254112189414475No Hit
GTCCTAAAGTGTGTATTTCTCATTT60160.2209326324402259No Hit
GTATCAACGCAGAGTACTTTTTTTT56920.20903399997502758No Hit
CTTTAGGACGTGAAATATGGCGAGG53690.19717209168410452No Hit
GTCCTACAGTGTGCATTTCTCATTT34960.12838771326646106No Hit
TATCAACGCAGAGTACTTTTTTTTT34370.12622098698421816No Hit
GGTATCAACGCAGAGTACTTTTTTT33910.12453167496755418No Hit
CTGAAGGACCTGGAATATGGCGAGA27560.10121182430273647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1850.013.24409819
TCCAACG3300.013.13604518
GCGTACA903.3564902E-612.4732852
TAGGACC11200.012.3679064
CGGTAGG1153.4981713E-711.5365571
CCGTGCC1002.129691E-611.3075919
TGTAGGA39150.011.1568782
AGGACGT39700.011.03720955
GGACGTG38750.010.9430326
ACGTGTC951.4895615E-510.9108338
ATTTAGA9650.010.7870711
TTTAGAA11000.010.7620742
CTGTAGG39500.010.7479821
GGTATCA14800.010.6191481
GTCCTAC37600.010.6125471
GGCGAGG16800.010.60164519
GTAGGAC41300.010.5868013
AGTCGTC2950.010.5409758
GACGTGG22900.010.4930457
TAGGACG43000.010.453144
Produced by FastQC (version 0.11.5)