FastQCFastQC Report
Thu 26 May 2016
SRR1512206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2723002
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT90300.33161929370599064No Hit
CTGTAGGACGTGGAATATGGCAAGA84500.31031927262631465No Hit
GTATCAACGCAGAGTACTTTTTTTT73800.2710244061517399No Hit
GTCCTAAAGTGTGTATTTCTCATTT67410.24755765878982094No Hit
CTTTAGGACGTGAAATATGGCGAGG64160.23562230215034732No Hit
GGTATCAACGCAGAGTACTTTTTTT46480.17069396203161072No Hit
TATCAACGCAGAGTACTTTTTTTTT46200.16966568515190222No Hit
GTCCTACAGTGTGCATTTCTCATTT39780.14608876526715736No Hit
CTGAAGGACCTGGAATATGGCGAGA33120.12163046519980522No Hit
CTGTAGGACCTGGAATATGGCGAGA31510.11571787314148135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT350.002175899216.27995518
TCCAACG3600.014.24495918
TAGCACG551.9595573E-413.8193854
CCAACGA2600.013.5156919
CTACCGT655.4509743E-513.1549914
TAGGACC12600.012.66776854
GCCGGTT2050.012.5077711
GGTATCA17400.012.0659591
CCGGTTT2150.011.92601312
GTCTACG657.7278505E-411.7453241
GGCGAGG19000.011.39701519
CCAACGT1251.8409992E-811.39701419
TGTAGGA42900.011.228252
CGGTTTC2200.011.22330113
TTGGCGA600.00589307911.07941217
AGGACCT24500.010.8193095
AGGACGT42550.010.8070515
GGACGTG42150.010.74708756
TTAGGAC23700.010.7435513
GTAGGAC43250.010.7200083
Produced by FastQC (version 0.11.5)