##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512206_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2723002 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.249762210971568 31.0 31.0 34.0 28.0 34.0 2 31.4456177410079 33.0 31.0 34.0 28.0 34.0 3 31.54145020826279 33.0 31.0 34.0 28.0 34.0 4 35.12281371809495 37.0 35.0 37.0 32.0 37.0 5 34.79370341997545 37.0 35.0 37.0 32.0 37.0 6 34.74107694375546 37.0 35.0 37.0 32.0 37.0 7 34.592059058348106 37.0 35.0 37.0 31.0 37.0 8 34.50959933191382 37.0 35.0 37.0 31.0 37.0 9 36.06267714823566 39.0 37.0 39.0 31.0 39.0 10 35.88706177960942 39.0 35.0 39.0 30.0 39.0 11 35.87831922268144 39.0 35.0 39.0 30.0 39.0 12 35.72464801715166 39.0 35.0 39.0 30.0 39.0 13 35.71892749252479 39.0 35.0 39.0 30.0 39.0 14 36.80350583657302 40.0 36.0 41.0 30.0 41.0 15 36.7555503080791 40.0 36.0 41.0 30.0 41.0 16 36.668787242903235 40.0 36.0 41.0 30.0 41.0 17 36.60914204249575 40.0 36.0 41.0 30.0 41.0 18 36.55892650831692 40.0 36.0 41.0 30.0 41.0 19 36.58771899543225 40.0 36.0 41.0 30.0 41.0 20 36.48511348871576 40.0 36.0 41.0 30.0 41.0 21 36.37707831283267 40.0 36.0 41.0 30.0 41.0 22 36.47222293630339 40.0 36.0 41.0 30.0 41.0 23 36.38127111180969 40.0 36.0 41.0 30.0 41.0 24 36.27953449905655 40.0 36.0 41.0 30.0 41.0 25 35.875885511652214 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0960754721904955 1101 2 0.11284769245862236 1101 3 0.24435887052962357 1101 4 0.2134646606313595 1101 5 0.2453357191644301 1101 6 0.20153584940887015 1101 7 0.18583805124643504 1101 8 0.07050648477547838 1101 9 0.04398999861071218 1101 10 0.08344255579841331 1101 11 0.0855740439380952 1101 12 0.14799374255706965 1101 13 0.08107213470805874 1101 14 -0.035519531312907304 1101 15 0.05685111902083406 1101 16 0.12708916048111973 1101 17 -0.011006658380892986 1101 18 0.1318527403096823 1101 19 0.09193472561314309 1101 20 0.05629919258964122 1101 21 -0.024131676980829297 1101 22 0.025395521402444388 1101 23 -0.053800756789335935 1101 24 -0.07536585517922134 1101 25 -0.03820584032023788 1102 1 0.4032403337623336 1102 2 0.40821462426859867 1102 3 0.3391805095424125 1102 4 0.3911615860035269 1102 5 0.4880961083827202 1102 6 0.4664610310579036 1102 7 0.3738445289991361 1102 8 0.4317753314865911 1102 9 0.62875437342921 1102 10 0.43360334726425265 1102 11 0.6182255436085242 1102 12 0.6850954706235939 1102 13 0.5288368029921173 1102 14 0.6182673266543759 1102 15 0.8504354396634497 1102 16 0.7187154052129472 1102 17 0.7248145765405809 1102 18 0.8733272587419307 1102 19 0.7989202812694245 1102 20 0.8308041039123424 1102 21 0.6127507969798671 1102 22 0.7213198841135693 1102 23 0.7389306699910065 1102 24 0.7617997899917341 1102 25 0.7333161703643682 1103 1 0.23475913629041045 1103 2 0.25256012140587103 1103 3 0.23310656146042064 1103 4 0.3016154387857455 1103 5 0.26313920402019164 1103 6 0.248360908287232 1103 7 0.3100217672181458 1103 8 0.32929921900760917 1103 9 0.4676645967591213 1103 10 0.32439665622531066 1103 11 0.27100952703485603 1103 12 0.32865763963103944 1103 13 0.32890242257330726 1103 14 0.2961357364617001 1103 15 0.3839744949303352 1103 16 0.48414024104034326 1103 17 0.23210190232381933 1103 18 0.41010622462349744 1103 19 0.4648923316445135 1103 20 0.3952141894231431 1103 21 0.4995853476528538 1103 22 0.7088343273910809 1103 23 0.8051694254432533 1103 24 0.7640768404200386 1103 25 0.8177031316426664 1104 1 0.2515152973442909 1104 2 0.17591679125030169 1104 3 0.2808854101112068 1104 4 0.15873231944304678 1104 5 0.24307689199180516 1104 6 0.33266514389013935 1104 7 0.36483715200334643 1104 8 0.27287578187705464 1104 9 0.3857643064615175 1104 10 0.3577078265539555 1104 11 0.4728426079481238 1104 12 0.46279552198643614 1104 13 0.6143709275784275 1104 14 0.7576563451513394 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-0.20522315811835412 2313 3 -0.28909701524901266 2313 4 -0.12001625033886398 2313 5 -0.2117761607465951 2313 6 -0.28481450593553603 2313 7 -0.27088673687138964 2313 8 -0.2710576961886062 2313 9 -0.2814761954170564 2313 10 -0.37702935542072424 2313 11 -0.4764165503170261 2313 12 -0.34855278100454967 2313 13 -0.3229429443009977 2313 14 -0.41584839960186315 2313 15 -0.2798418019115374 2313 16 -0.2758592862930982 2313 17 -0.25480646749725366 2313 18 -0.45632378845664334 2313 19 -0.38281099581121936 2313 20 -0.32707862257802134 2313 21 -0.44956326170108696 2313 22 -0.1996601529931965 2313 23 -0.1784252790810612 2313 24 -0.2826963727347902 2313 25 -0.23980545589069635 2314 1 -0.3504218448622005 2314 2 -0.3812582177740289 2314 3 -0.41302843599791217 2314 4 -0.3656309504413713 2314 5 -0.445185179413059 2314 6 -0.2958657919995602 2314 7 -0.30320157630495004 2314 8 -0.3252724739439401 2314 9 -0.38035217887061634 2314 10 -0.405124408112556 2314 11 -0.3889331138166199 2314 12 -0.3896475883914192 2314 13 -0.48931342193086635 2314 14 -0.4229719905198408 2314 15 -0.4230755277147509 2314 16 -0.4153453155531537 2314 17 -0.39037411561839264 2314 18 -0.3291538007868766 2314 19 -0.32724947760639367 2314 20 -0.4585922949448218 2314 21 -0.4285141471003513 2314 22 -0.24883139588787628 2314 23 -0.4226365965744421 2314 24 -0.4313048221637956 2314 25 -0.5075072408768477 2315 1 -0.19198613658741692 2315 2 -0.18072343349823683 2315 3 -0.17403544276103133 2315 4 -0.1480788432195368 2315 5 -0.15406963827660292 2315 6 -0.262661672936801 2315 7 -0.1719903511122638 2315 8 -0.26207148184781204 2315 9 -0.24841406024910384 2315 10 -0.2917393628725904 2315 11 -0.26123891206608363 2315 12 -0.10798256006065543 2315 13 -0.27506411006001485 2315 14 -0.3739684374548773 2315 15 -0.34218702007501633 2315 16 -0.3945648538736535 2315 17 -0.2719222616415635 2315 18 -0.3702628016789262 2315 19 -0.4943348134802221 2315 20 -0.408973902767066 2315 21 -0.42955328807714466 2315 22 -0.19439107519696108 2315 23 -0.20095678111599824 2315 24 -0.016629411966960106 2315 25 -0.08413324949200529 2316 1 -0.7236419722895846 2316 2 -0.7875577984133244 2316 3 -0.779084375561343 2316 4 -0.7581898786637709 2316 5 -0.8519126620278996 2316 6 -0.8037328269365034 2316 7 -0.8885547107157379 2316 8 -0.8250058081156766 2316 9 -1.0249475715097134 2316 10 -1.0692351910222584 2316 11 -1.1261422979365676 2316 12 -1.3301442711817018 2316 13 -1.2614059967364213 2316 14 -1.4961476299754821 2316 15 -1.3703533511682409 2316 16 -1.2599307521090637 2316 17 -1.3974800341954534 2316 18 -1.3656985997552127 2316 19 -1.2801783738802541 2316 20 -1.2557393007052013 2316 21 -1.262332446486063 2316 22 -1.320020744346074 2316 23 -1.2923683744531829 2316 24 -1.3249998442793967 2316 25 -1.215985893724465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28591.0 3 0.0 4 509.0 5 4834.0 6 6347.0 7 5246.0 8 4096.0 9 3782.0 10 3536.0 11 3467.0 12 3257.0 13 3279.0 14 3315.0 15 3282.0 16 3430.0 17 3609.0 18 3862.0 19 4328.0 20 5337.0 21 6864.0 22 9126.0 23 11686.0 24 14369.0 25 17539.0 26 20609.0 27 23881.0 28 28280.0 29 34801.0 30 45181.0 31 58985.0 32 77319.0 33 101832.0 34 134234.0 35 179078.0 36 259712.0 37 448542.0 38 1156857.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.5562732515996 19.676003888100897 13.544240058874848 25.22348280142465 2 14.091396187002433 20.34765306819459 42.37183814040533 23.189112604397646 3 18.82697845980282 24.350073925762818 30.07114941524097 26.751798199193388 4 12.261724376258263 17.12716332929612 38.592333020688194 32.01877927375742 5 12.692339483665954 38.110412093715276 36.342826703893174 12.854421718725604 6 31.601849723209895 37.77140082893806 17.945267759627058 12.681481688224979 7 27.960317326245075 31.62381077942653 22.164765211336608 18.251106682991786 8 24.271961607079245 38.88803607195294 20.338508748800038 16.501493572167778 9 25.93788032472984 15.70325692011978 20.678464430066523 37.68039832508386 10 14.808986552341864 29.484774524587205 34.711138662402746 20.99510026066819 11 34.05946818988748 22.372440416863448 25.286907611525812 18.28118378172326 12 23.466967706964592 24.555215163264663 32.30375886613378 19.67405826363697 13 29.04162391360711 22.257971165647326 26.030498692252152 22.669906228493407 14 20.04783690941101 23.12021805345718 27.811474247907274 29.02047078922454 15 22.058742520203804 32.77643571323121 23.315333591381865 21.849488175183122 16 21.069283092704303 28.72943170809276 29.1577824768399 21.043502722363037 17 20.77941918514933 29.594543081496084 28.696196330373606 20.929841402980976 18 20.603290045324975 27.828661161468116 31.487564092865156 20.080484700341756 19 23.178389145509257 27.037952965146555 28.013200137201515 21.770457752142672 20 23.073394731256165 27.94375472364692 28.946545026408355 20.036305518688565 21 22.049598200809253 27.264210602856696 27.691349473852757 22.99484172248129 22 22.44082082936406 29.074712394629167 28.000493572902258 20.483973203104515 23 21.329584039967653 28.745480172251064 28.658811120961353 21.26612466681993 24 22.74181216172445 27.11305390153955 29.52711749752663 20.61801643920937 25 21.99796386222722 28.07097247614773 29.102079402027766 20.828984259597284 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 370.0 1 370.0 2 497.0 3 624.0 4 624.0 5 624.0 6 1648.5 7 2673.0 8 2673.0 9 2673.0 10 4085.0 11 5497.0 12 5497.0 13 5497.0 14 8893.5 15 12290.0 16 12290.0 17 12290.0 18 22526.0 19 32762.0 20 32762.0 21 32762.0 22 53534.5 23 74307.0 24 74307.0 25 74307.0 26 108570.0 27 142833.0 28 142833.0 29 142833.0 30 188063.0 31 233293.0 32 233293.0 33 233293.0 34 261283.0 35 289273.0 36 289273.0 37 289273.0 38 305521.5 39 321770.0 40 321770.0 41 321770.0 42 336770.5 43 351771.0 44 351771.0 45 351771.0 46 353262.5 47 354754.0 48 354754.0 49 354754.0 50 335125.0 51 315496.0 52 315496.0 53 315496.0 54 274669.5 55 233843.0 56 233843.0 57 233843.0 58 197792.0 59 161741.0 60 161741.0 61 161741.0 62 130566.5 63 99392.0 64 99392.0 65 99392.0 66 76031.0 67 52670.0 68 52670.0 69 52670.0 70 38407.0 71 24144.0 72 24144.0 73 24144.0 74 16558.0 75 8972.0 76 8972.0 77 8972.0 78 6109.5 79 3247.0 80 3247.0 81 3247.0 82 2106.5 83 966.0 84 966.0 85 966.0 86 602.5 87 239.0 88 239.0 89 239.0 90 150.0 91 61.0 92 61.0 93 61.0 94 36.5 95 12.0 96 12.0 97 12.0 98 7.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4468230284076178 2 0.0 3 0.0 4 0.0 5 0.03404330955320635 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007344834855060702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2723002.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.27848519992449 #Duplication Level Percentage of deduplicated Percentage of total 1 83.80097150945163 58.8940533596629 2 11.371055955846538 15.982811754009482 3 2.6269521084934144 5.538546446329945 4 0.9131334193884801 2.5669453400019897 5 0.3932949637889519 1.3820087145923345 6 0.22598997704134316 0.9529339954098783 7 0.132769339399554 0.6531579637796715 8 0.09487364953933206 0.5334061100010217 9 0.06542003587513122 0.4137858920726044 >10 0.32961549854806643 4.064915469299342 >50 0.023038102799469173 1.1267947123130178 >100 0.018403293334715562 2.6814446803918788 >500 0.002084719299380061 1.0105590322446905 >1k 0.002136837281704733 2.80616017154821 >5k 2.605899124030162E-4 1.3924763583432096 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 9030 0.33161929370599064 No Hit CTGTAGGACGTGGAATATGGCAAGA 8450 0.31031927262631465 No Hit GTATCAACGCAGAGTACTTTTTTTT 7380 0.2710244061517399 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6741 0.24755765878982094 No Hit CTTTAGGACGTGAAATATGGCGAGG 6416 0.23562230215034732 No Hit GGTATCAACGCAGAGTACTTTTTTT 4648 0.17069396203161072 No Hit TATCAACGCAGAGTACTTTTTTTTT 4620 0.16966568515190222 No Hit GTCCTACAGTGTGCATTTCTCATTT 3978 0.14608876526715736 No Hit CTGAAGGACCTGGAATATGGCGAGA 3312 0.12163046519980522 No Hit CTGTAGGACCTGGAATATGGCGAGA 3151 0.11571787314148135 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.672417427530351E-5 0.0 6 0.0 0.0 0.0 3.672417427530351E-5 0.0 7 0.0 0.0 0.0 3.672417427530351E-5 0.0 8 0.0 0.0 0.0 3.672417427530351E-5 0.0 9 0.0 0.0 0.0 3.672417427530351E-5 0.0 10 3.672417427530351E-5 0.0 0.0 7.344834855060702E-5 0.0 11 3.672417427530351E-5 0.0 0.0 7.344834855060702E-5 0.0 12 3.672417427530351E-5 0.0 0.0 7.344834855060702E-5 0.0 13 3.672417427530351E-5 0.0 0.0 1.1017252282591052E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGTT 35 0.0021758992 16.279955 18 TCCAACG 360 0.0 14.244959 18 TAGCACG 55 1.9595573E-4 13.819385 4 CCAACGA 260 0.0 13.51569 19 CTACCGT 65 5.4509743E-5 13.154991 4 TAGGACC 1260 0.0 12.6677685 4 GCCGGTT 205 0.0 12.50777 11 GGTATCA 1740 0.0 12.065959 1 CCGGTTT 215 0.0 11.926013 12 GTCTACG 65 7.7278505E-4 11.745324 1 GGCGAGG 1900 0.0 11.397015 19 CCAACGT 125 1.8409992E-8 11.397014 19 TGTAGGA 4290 0.0 11.22825 2 CGGTTTC 220 0.0 11.223301 13 TTGGCGA 60 0.005893079 11.079412 17 AGGACCT 2450 0.0 10.819309 5 AGGACGT 4255 0.0 10.807051 5 GGACGTG 4215 0.0 10.7470875 6 TTAGGAC 2370 0.0 10.743551 3 GTAGGAC 4325 0.0 10.720008 3 >>END_MODULE