FastQCFastQC Report
Thu 26 May 2016
SRR1512205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2218195
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT52650.23735514686490594No Hit
CTGTAGGACGTGGAATATGGCAAGA44000.1983594769621246No Hit
GTATCAACGCAGAGTACTTTTTTTT38810.1749620750204558No Hit
GTCCTAAAGTGTGTATTTCTCATTT30740.13858114367762978No Hit
GTCCTACAGTGTGCATTTCTCATTT27780.12523696068199594No Hit
CTTTAGGACGTGAAATATGGCGAGG27460.12379434630408959No Hit
GGTATCAACGCAGAGTACTTTTTTT26130.11779848029591627No Hit
TATCAACGCAGAGTACTTTTTTTTT22930.10337233651685265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9700.013.7916844
CGTAGGA851.7328675E-613.2304752
TACCGAG655.898441E-513.0445717
TCCAACG2000.012.72017918
GTCCTAC26000.012.1502961
TGTAGGA24150.012.0227262
TCTCGTA600.003398141111.9274742
GTAGGAC25650.011.7901233
TCCTACA28000.011.5014932
CTGTAGG24600.011.3872071
AATCCCG3850.011.25787619
GGACCTG16750.011.2490496
AGGACCT17900.011.1052945
GGCGAGG10100.011.10158419
AGGACGT22450.011.0786865
GACGTGA11100.011.0336967
TTAGGAC13250.011.0208853
TAGGACT3850.011.0116514
TCGTCTA600.006250198510.992002513
CGGTAGG750.001437871410.9051211
Produced by FastQC (version 0.11.5)