FastQCFastQC Report
Thu 26 May 2016
SRR1512205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2218195
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT61980.27941637232073824No Hit
GTATCAACGCAGAGTACTTTTTTTT54180.24425264685927073No Hit
CTGTAGGACGTGGAATATGGCAAGA51910.23401910111599747No Hit
GGTATCAACGCAGAGTACTTTTTTT37720.1700481697957123No Hit
GTCCTAAAGTGTGTATTTCTCATTT35500.1600400325489869No Hit
TATCAACGCAGAGTACTTTTTTTTT33430.15070812079190513No Hit
CTTTAGGACGTGAAATATGGCGAGG31710.14295406851065845No Hit
GTCCTACAGTGTGCATTTCTCATTT31620.14254833321687227No Hit
CTGTAGGACCTGGAATATGGCGAGA23050.10391331690856756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10100.013.7329674
CGGTTTC1900.012.99588213
GCCGGTT1550.012.8668911
CCGGTTT1700.012.8488712
TCCAACG2750.012.08707918
CCAACGA2050.012.0460519
TGTAGGA28250.011.8374212
GTAGGAC26250.011.7983473
CCCGTTT2100.011.7581812
GGACGGT658.0471486E-411.6886046
GATATAC7300.011.6317481
AGGACCT17500.011.4002445
GTCCTAC25800.011.389611
TAGGACA6400.011.2814914
AGGACGA855.3238444E-511.176715
AGGACGT23600.011.1506625
TTAGGAC12200.011.1354853
GGACGTG23250.011.0696126
CTGTAGG28950.011.0401281
CTAGTAC1900.011.0002363
Produced by FastQC (version 0.11.5)