FastQCFastQC Report
Thu 26 May 2016
SRR1512204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482442
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59240.2386359882728378No Hit
TATCAACGCAGAGTACTTTTTTTTT37470.1509400823866177No Hit
GGTATCAACGCAGAGTACTTTTTTT35750.14401142101205183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT604.3924362E-412.5612567
GGTATCA15500.012.2723971
TAAGACG550.003267993711.9900484
TCCGTTA658.604673E-411.59570517
GTCCTAA4950.011.1567241
TTCCGTT700.001598815910.76744216
CGGAACC1002.6322852E-510.3628274
GTATCAA31450.010.3082931
ACCGTGC2200.010.2773918
TACCGAT1304.310823E-710.145637
TTAGGAC4750.010.1149143
GATATAC4550.010.1146131
GTATAAG3050.010.0593421
GTATAAA2850.010.0475761
GTCCTAC6550.09.9927911
GTATTAG1851.880835E-99.9505921
GTTAGAC1054.502643E-59.869363
GTGCCGA1252.8609884E-69.7977811
CGGTAGC1052.1446649E-49.7399981
GGCGAGG3500.09.69128319
Produced by FastQC (version 0.11.5)