FastQCFastQC Report
Thu 26 May 2016
SRR1512204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482442
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82870.33382451634318144No Hit
TATCAACGCAGAGTACTTTTTTTTT53050.2137008639073944No Hit
GGTATCAACGCAGAGTACTTTTTTT52030.20959200658061697No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26420.10642746134652895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC1251.7183993E-811.4500991
GGTATCA18150.011.3028981
CTAGTAC1158.8154593E-710.7398143
GATATAC4700.010.55682851
ACAACGC1002.4089588E-510.4464558
TAGGACG6700.010.3514044
GACGTGA3500.010.3107867
GTAGGAC6450.010.3107343
TCCGCGC951.6503606E-49.99660916
TTAGGAC4700.09.9048853
ACCGATC1356.476439E-79.848518
TGTAGGA7150.09.8327942
AATCCCG3000.09.81452419
GTATCAA35950.09.7408121
GTATTAG2002.4374458E-109.5417491
AGGACGT7000.09.5006055
TAGGACC3000.09.5006054
TCTATAC2600.09.5006053
ACTGCGA1401.0640033E-69.4967788
TGCGCAA900.00111746869.49677810
Produced by FastQC (version 0.11.5)