##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512204_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2482442 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14535485622625 31.0 31.0 34.0 28.0 34.0 2 31.323848855280406 31.0 31.0 34.0 28.0 34.0 3 31.416010525120022 33.0 31.0 34.0 28.0 34.0 4 35.02495486299378 37.0 35.0 37.0 32.0 37.0 5 34.667957196985874 37.0 35.0 37.0 32.0 37.0 6 34.610409024662005 37.0 35.0 37.0 31.0 37.0 7 34.44697076507729 37.0 35.0 37.0 30.0 37.0 8 34.3653527454015 37.0 35.0 37.0 30.0 37.0 9 35.90115176910478 39.0 35.0 39.0 30.0 39.0 10 35.729690764174954 39.0 35.0 39.0 30.0 39.0 11 35.725835689212474 39.0 35.0 39.0 30.0 39.0 12 35.55006642652678 39.0 35.0 39.0 30.0 39.0 13 35.527203455307315 39.0 35.0 39.0 30.0 39.0 14 36.594421944198494 40.0 36.0 41.0 30.0 41.0 15 36.51000788739475 40.0 36.0 41.0 30.0 41.0 16 36.41857976943671 40.0 36.0 41.0 30.0 41.0 17 36.350686944548954 40.0 36.0 41.0 29.0 41.0 18 36.289462956234225 39.0 36.0 41.0 29.0 41.0 19 36.30592577792351 40.0 36.0 41.0 29.0 41.0 20 36.18668271000894 39.0 36.0 41.0 29.0 41.0 21 36.08787959597848 39.0 36.0 41.0 29.0 41.0 22 36.174149889503965 39.0 36.0 41.0 29.0 41.0 23 36.06748959290892 39.0 36.0 41.0 29.0 41.0 24 35.944540093988095 39.0 36.0 41.0 29.0 41.0 25 35.50266632614176 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09715183930881821 1101 2 0.15600066725557227 1101 3 0.20531867590696606 1101 4 0.09050465604931901 1101 5 0.23215022338646207 1101 6 0.18217079146598536 1101 7 0.24271980148405703 1101 8 0.10737401104122313 1101 9 0.27033337901758614 1101 10 0.39031232114795955 1101 11 0.2988665996763942 1101 12 0.3721165373268107 1101 13 0.22196759789543563 1101 14 0.21222008886511645 1101 15 0.11533873151095264 1101 16 0.14063753319272365 1101 17 0.174444481230438 1101 18 0.23520655597045703 1101 19 0.24246572804393907 1101 20 0.21262805925081096 1101 21 0.2668576717236206 1101 22 0.1053799355694025 1101 23 -0.0013581057829199494 1101 24 -0.15750532822004004 1101 25 0.1328983780579165 1102 1 0.20954533482758464 1102 2 0.3539960367102637 1102 3 0.2615020439499993 1102 4 0.2854062084842326 1102 5 0.3407729658017189 1102 6 0.43204843492950573 1102 7 0.38771889011206895 1102 8 0.4062410011479898 1102 9 0.4583536851610077 1102 10 0.5218647948442126 1102 11 0.485158983321341 1102 12 0.578981301359029 1102 13 0.5414496283546555 1102 14 0.6196458314393709 1102 15 0.7600273719378592 1102 16 0.6619523908829592 1102 17 0.8138347539827464 1102 18 0.7816556955965268 1102 19 0.7866447757959563 1102 20 0.7942526670699976 1102 21 0.7738544877389515 1102 22 0.7856089808005962 1102 23 0.776357297954867 1102 24 0.8796274567388309 1102 25 0.8435011245840371 1103 1 0.4717063335361509 1103 2 0.40177907588374495 1103 3 0.39365873788026207 1103 4 0.4313381929870346 1103 5 0.4023312955477323 1103 6 0.3945968636854289 1103 7 0.45444295382279165 1103 8 0.4350830157842296 1103 9 0.5410054285962218 1103 10 0.4318949282923441 1103 11 0.4236006535753205 1103 12 0.49374669094146384 1103 13 0.5879412340369612 1103 14 0.38503541387587603 1103 15 0.5826705058164663 1103 16 0.7394384003534711 1103 17 0.47203535665170904 1103 18 0.7730461389886898 1103 19 0.7285302686930706 1103 20 0.6168958009486047 1103 21 0.7626620641487634 1103 22 0.8144509954368431 1103 23 0.8297365489234423 1103 24 0.7806175557487336 1103 25 0.6119040518332852 1104 1 0.4959328613456542 1104 2 0.45609209060280875 1104 3 0.4600345969019557 1104 4 0.4114129850971864 1104 5 0.6056469759529861 1104 6 0.6096530305761334 1104 7 0.6647061641680949 1104 8 0.5966650991695204 1104 9 0.7978270460461374 1104 10 0.6614815876660671 1104 11 0.9295724641031882 1104 12 0.9207004277711661 1104 13 1.0176478447384767 1104 14 1.1195139577868929 1104 15 1.1427066914370485 1104 16 1.2813304459786607 1104 17 1.309406292936437 1104 18 1.2299240006879018 1104 19 1.2523204030148989 1104 20 1.109627278343055 1104 21 1.2190499274042494 1104 22 1.2538762119726528 1104 23 1.2269707516157027 1104 24 1.1741214359648637 1104 25 1.2514963629349722 1105 1 0.054433624405174186 1105 2 0.18122440092301417 1105 3 0.09914437246445118 1105 4 0.04575941411864903 1105 5 0.11789093098147418 1105 6 0.0526752363081755 1105 7 0.18343560006409376 1105 8 0.06124758660353535 1105 9 0.06483102235628735 1105 10 0.2639415795454312 1105 11 0.2492074206298014 1105 12 0.1766083635901552 1105 13 0.2783663807842842 1105 14 0.29640883058696943 1105 15 0.2320039066198376 1105 16 0.38444831417238134 1105 17 0.06521919634290185 1105 18 0.27969823307802955 1105 19 0.42796480327811537 1105 20 0.3805431597818085 1105 21 0.42600987027979187 1105 22 0.383291456361718 1105 23 0.36261928220108786 1105 24 0.39127084861905104 1105 25 0.38090565685990185 1106 1 -0.3211927552857752 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13 0.33183160202695916 1107 14 0.2826022544702127 1107 15 0.25471871659157586 1107 16 0.34616057215525586 1107 17 0.40424880785005257 1107 18 0.5217812676247888 1107 19 0.4177963931039059 1107 20 0.45901154464704064 1107 21 0.35327027859801063 1107 22 0.46555882464956255 1107 23 0.3572305784427101 1107 24 0.36913867246854437 1107 25 0.23426120585386911 1108 1 0.029501797369722027 1108 2 0.034701222115067765 1108 3 0.023788899149295162 1108 4 -0.004542494724361745 1108 5 -0.05429449948209708 1108 6 0.07083846186330334 1108 7 0.03800805818641351 1108 8 0.12791100693856094 1108 9 0.06887270525461275 1108 10 0.15350137697434008 1108 11 0.10382857372680121 1108 12 0.15720341618072808 1108 13 0.21623159309437767 1108 14 0.12225125364891198 1108 15 0.1841367465107595 1108 16 0.15589845051831475 1108 17 0.2223578796307919 1108 18 0.24790041461289292 1108 19 0.23152927542459167 1108 20 0.274339420007756 1108 21 0.39581898597905507 1108 22 0.35344143688372753 1108 23 0.34095001846910833 1108 24 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-0.624932688912665 2314 15 -0.5850835657863485 2314 16 -0.6101226019424075 2314 17 -0.6607281914422316 2314 18 -0.6114901407262394 2314 19 -0.671789151835938 2314 20 -0.727311880689129 2314 21 -0.7513011120601618 2314 22 -0.5220318512173847 2314 23 -0.5936629105877245 2314 24 -0.5024377606524766 2314 25 -0.7684435889090508 2315 1 -0.12055158886312967 2315 2 -0.16821879378672833 2315 3 -0.12831737595749004 2315 4 -0.2300676732345721 2315 5 -0.1659318997322643 2315 6 -0.16197599074814661 2315 7 -0.2710812616581464 2315 8 -0.10827502012757861 2315 9 -0.2130322706730965 2315 10 -0.2522826163957319 2315 11 -0.17516753694924603 2315 12 -0.2371068148687243 2315 13 -0.17365188688949473 2315 14 -0.47269938404850365 2315 15 -0.4033104447516962 2315 16 -0.5702418674377725 2315 17 -0.4265184143151757 2315 18 -0.3838587758327918 2315 19 -0.4693158925224523 2315 20 -0.4901880804613725 2315 21 -0.3986000993710448 2315 22 -0.30648412468411834 2315 23 -0.3027872801989204 2315 24 -0.22127011789991258 2315 25 -0.26217172865201377 2316 1 -0.7550826236449666 2316 2 -0.6900253305334729 2316 3 -0.7892600353854249 2316 4 -0.7917892739879093 2316 5 -0.8631101992364378 2316 6 -0.8317798305241269 2316 7 -0.9253274418100119 2316 8 -0.8328190028471383 2316 9 -1.1094198579993986 2316 10 -1.1841314456089194 2316 11 -1.1515406481920678 2316 12 -1.2424135075276368 2316 13 -1.0955466017767321 2316 14 -1.445255228483262 2316 15 -1.4283854581229676 2316 16 -1.404394169878465 2316 17 -1.3495602710535763 2316 18 -1.3612158890415529 2316 19 -1.2434476888990957 2316 20 -1.1274372528878445 2316 21 -1.2795730326055335 2316 22 -1.256873079081359 2316 23 -1.0803362376524461 2316 24 -1.167652852330896 2316 25 -1.2166340175252515 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28402.0 3 1.0 4 470.0 5 4760.0 6 6084.0 7 5127.0 8 4165.0 9 3771.0 10 3593.0 11 3412.0 12 3410.0 13 3306.0 14 3238.0 15 3240.0 16 3466.0 17 3577.0 18 3913.0 19 4392.0 20 5363.0 21 6995.0 22 8819.0 23 11514.0 24 14053.0 25 17344.0 26 20231.0 27 23164.0 28 27526.0 29 34127.0 30 44228.0 31 57066.0 32 74041.0 33 96993.0 34 127192.0 35 169177.0 36 243139.0 37 415072.0 38 998071.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0760148306225 18.463987427231746 13.587791078076842 24.872206664068923 2 15.836623776104336 20.052915637102497 39.018635682122685 25.091824904670485 3 18.877822724559124 23.844706140163595 29.667641781761667 27.609829353515607 4 13.459569246733661 16.59474823580974 36.23577106736028 33.709911450096314 5 14.28699799488082 36.79619349722443 34.61264450075112 14.30416400714364 6 33.2247843051318 35.710763836577044 17.282860989300055 13.781590868991097 7 29.017435251256625 31.32286675781348 21.184784981884775 18.474913009045128 8 26.448714612466272 35.2411053309604 19.78060313191607 18.529576924657253 9 26.68026080770467 15.550977626063368 19.431068278735214 38.33769328749675 10 16.010686251682817 28.369766544394594 32.63621063452842 22.98333656939417 11 35.132865138440295 22.552833057126815 22.888309172983696 19.425992631449194 12 23.52216084001157 24.268885234780914 30.275470685719945 21.93348323948757 13 28.971150181957928 21.101399347900173 25.633106433100956 24.294344037040947 14 22.31081330399663 20.817082534053164 26.739234995218418 30.13286916673179 15 24.199840318525066 28.525419727832514 23.393054097537828 23.881685856104593 16 23.775459809332908 27.461507660601942 25.552742017738982 23.21029051232617 17 22.86836913007434 27.353992560551266 26.56094281356825 23.216695495806146 18 22.739504085090406 27.003007522431545 27.59017129101103 22.667317101467024 19 23.940861458193183 26.575525228786816 26.918775947232604 22.564837365787398 20 24.06142822269362 26.280936271622863 27.15346420983854 22.50417129584498 21 24.221834790097816 25.87738202946937 26.833295601669647 23.067487578763167 22 24.135307088745677 25.879960136027346 26.966712616045008 23.01802015918197 23 24.005918365867156 25.836454587861468 26.830274383047016 23.327352663224357 24 23.764140310226782 25.853494260893108 26.918735664317634 23.463629764562473 25 23.85253575779452 25.915981268652388 26.96337525218994 23.268107721363148 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 563.0 1 563.0 2 678.5 3 794.0 4 794.0 5 794.0 6 1904.5 7 3015.0 8 3015.0 9 3015.0 10 3897.0 11 4779.0 12 4779.0 13 4779.0 14 7115.5 15 9452.0 16 9452.0 17 9452.0 18 16607.5 19 23763.0 20 23763.0 21 23763.0 22 36562.5 23 49362.0 24 49362.0 25 49362.0 26 70554.0 27 91746.0 28 91746.0 29 91746.0 30 117865.0 31 143984.0 32 143984.0 33 143984.0 34 166837.0 35 189690.0 36 189690.0 37 189690.0 38 214875.0 39 240060.0 40 240060.0 41 240060.0 42 264531.5 43 289003.0 44 289003.0 45 289003.0 46 307154.5 47 325306.0 48 325306.0 49 325306.0 50 329168.5 51 333031.0 52 333031.0 53 333031.0 54 309491.5 55 285952.0 56 285952.0 57 285952.0 58 248275.0 59 210598.0 60 210598.0 61 210598.0 62 175694.0 63 140790.0 64 140790.0 65 140790.0 66 110486.5 67 80183.0 68 80183.0 69 80183.0 70 59092.5 71 38002.0 72 38002.0 73 38002.0 74 26710.5 75 15419.0 76 15419.0 77 15419.0 78 10227.0 79 5035.0 80 5035.0 81 5035.0 82 3255.0 83 1475.0 84 1475.0 85 1475.0 86 914.5 87 354.0 88 354.0 89 354.0 90 216.0 91 78.0 92 78.0 93 78.0 94 42.0 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4458915857852872 2 0.0 3 0.0 4 0.0 5 0.031984634484914454 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007492622184123536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2482442.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.66650704855807 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7262595165061 50.90952215930754 2 13.14294659812775 16.998168976533012 3 3.8961064210117913 7.55842780008874 4 1.5949830219839356 4.125679233338186 5 0.8079706757123838 2.6124320697991554 6 0.49749219405537165 1.9302649484090588 7 0.27913112249022703 1.2635304289990335 8 0.2070293001715848 1.0710289359043068 9 0.13449614492945644 0.7827656313676126 >10 0.6618743493294706 7.354853785686115 >50 0.0331793245758822 1.4746144274666635 >100 0.01666415624631321 1.9733699875409134 >500 9.335874298493366E-4 0.4587857217649682 >1k 7.468699438780798E-4 0.7300967622427377 >5k 1.8671748596951995E-4 0.7564591315519436 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8287 0.33382451634318144 No Hit TATCAACGCAGAGTACTTTTTTTTT 5305 0.2137008639073944 No Hit GGTATCAACGCAGAGTACTTTTTTT 5203 0.20959200658061697 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2642 0.10642746134652895 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.028291496840611E-5 0.0 0.0 0.0 0.0 6 4.028291496840611E-5 0.0 0.0 0.0 0.0 7 4.028291496840611E-5 0.0 0.0 0.0 0.0 8 4.028291496840611E-5 0.0 0.0 0.0 0.0 9 4.028291496840611E-5 0.0 0.0 0.0 0.0 10 4.028291496840611E-5 0.0 0.0 0.0 0.0 11 4.028291496840611E-5 0.0 0.0 0.0 0.0 12 4.028291496840611E-5 0.0 0.0 0.0 0.0 13 4.028291496840611E-5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAC 125 1.7183993E-8 11.450099 1 GGTATCA 1815 0.0 11.302898 1 CTAGTAC 115 8.8154593E-7 10.739814 3 GATATAC 470 0.0 10.5568285 1 ACAACGC 100 2.4089588E-5 10.446455 8 TAGGACG 670 0.0 10.351404 4 GACGTGA 350 0.0 10.310786 7 GTAGGAC 645 0.0 10.310734 3 TCCGCGC 95 1.6503606E-4 9.996609 16 TTAGGAC 470 0.0 9.904885 3 ACCGATC 135 6.476439E-7 9.84851 8 TGTAGGA 715 0.0 9.832794 2 AATCCCG 300 0.0 9.814524 19 GTATCAA 3595 0.0 9.740812 1 GTATTAG 200 2.4374458E-10 9.541749 1 AGGACGT 700 0.0 9.500605 5 TAGGACC 300 0.0 9.500605 4 TCTATAC 260 0.0 9.500605 3 ACTGCGA 140 1.0640033E-6 9.496778 8 TGCGCAA 90 0.0011174686 9.496778 10 >>END_MODULE