FastQCFastQC Report
Thu 26 May 2016
SRR1512203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233519
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46410.20778869577559No Hit
GTATCAACGCAGAGTACTTTTTTTT41600.18625317268400224No Hit
CTGTAGGACGTGGAATATGGCAAGA40600.1817759329560214No Hit
GTCCTAAAGTGTGTATTTCTCATTT36350.16274766411210292No Hit
CTTTAGGACGTGAAATATGGCGAGG33140.14837572458528447No Hit
TATCAACGCAGAGTACTTTTTTTTT25110.11242348956959847No Hit
GGTATCAACGCAGAGTACTTTTTTT24940.11166235881584173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG751.0614149E-613.8196844
CGGTAGG902.2431595E-713.6176381
CACCGTC854.3236523E-612.1938397
TAGGACC7750.012.1580814
GGTATCA13900.011.9031871
TGTAGGA22900.011.7742022
ACGAAAT1850.011.20565212
AAGACGT1105.526217E-711.1356965
GTAGGAC24350.011.1057953
TCCAACG2600.010.87261618
GTCCTAC20850.010.7765481
GATATAC5700.010.7507671
CTGTAGG23650.010.6666691
ACCGTCC804.0701704E-410.5998528
AGGACGT23800.010.4914525
GGACGTG23350.010.411176
GTCCTAA13450.010.327131
GGCGAGG9950.010.32261219
CCAACGA1651.0713848E-910.27956419
GACGTGG13550.010.2222097
Produced by FastQC (version 0.11.5)