FastQCFastQC Report
Thu 26 May 2016
SRR1512203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233519
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT53880.24123367654360675No Hit
GTATCAACGCAGAGTACTTTTTTTT53400.23908460147417593No Hit
CTGTAGGACGTGGAATATGGCAAGA49170.22014587742481706No Hit
GTCCTAAAGTGTGTATTTCTCATTT41810.1871933930268782No Hit
CTTTAGGACGTGAAATATGGCGAGG38670.17313486028101843No Hit
GGTATCAACGCAGAGTACTTTTTTT31850.14260008533618923No Hit
TATCAACGCAGAGTACTTTTTTTTT30940.13852579718372668No Hit
GTCCTACAGTGTGCATTTCTCATTT23970.10731943627970034No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTA400.00512785714.3129571
TTGATCG400.005291505314.245359
TAGGACC8750.012.0518134
GACCGAG802.87727E-511.8711247
GGTATCA13850.011.84995651
TCCAACG2250.011.81836418
GGACCGT658.047841E-411.6884916
TGTAGGA25350.011.6177262
CGGTTTC1604.5474735E-1111.27756913
GTAGGAC25250.011.1369883
CTGTAGG26250.010.832411
GTCTTAT2001.8189894E-1210.4961691
CCAACGA1451.45064405E-810.47980619
TAGACTG2550.010.43170455
GCCGGTT1553.6834535E-910.41595411
AGGACGT25100.010.3708495
GGCGAGG10700.010.29616519
GACGTGG12500.010.2566517
AGGACCT17100.010.166995
TAGGACG26700.010.105194
Produced by FastQC (version 0.11.5)