FastQCFastQC Report
Thu 26 May 2016
SRR1512202_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512202_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447981
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT57460.2347240440183155No Hit
GTATCAACGCAGAGTACTTTTTTTT54070.22087589732109847No Hit
CTGTAGGACGTGGAATATGGCAAGA50740.20727285056542513No Hit
GTCCTAAAGTGTGTATTTCTCATTT39790.16254211123370646No Hit
CTTTAGGACGTGAAATATGGCGAGG36730.15004201421497962No Hit
TATCAACGCAGAGTACTTTTTTTTT33050.13500921780030156No Hit
GGTATCAACGCAGAGTACTTTTTTT32660.13341606818026772No Hit
GTCCTACAGTGTGCATTTCTCATTT24870.10159392576984871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8200.012.7527974
GTCGAGG1354.274625E-1011.8641919
GGTATCA13900.011.4800481
TGTAGAA10800.010.892012
CTGTAGG26450.010.8671231
TGTAGGA27250.010.848392
GTCCTAC27200.010.79311851
CCGTGCG700.00160009110.76649
GTAGGAC28600.010.5739033
AGGACGT30200.010.5440185
GACGTGG15650.010.3540557
GTAGAAC10750.010.3411753
GGACGTG29300.010.3212836
CCAACGA1651.0732037E-910.27806919
GTCCTAA14000.010.2290171
GTATCAA27250.010.2102491
TAGGACG32200.010.1231724
GGCGAGG11450.010.1209819
CCTACAC3450.010.1036663
CTAGGAC3550.010.08443453
Produced by FastQC (version 0.11.5)