FastQCFastQC Report
Thu 26 May 2016
SRR1512202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447981
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67670.27643188407099567No Hit
GTCCTACAGTGGACATTTCTAAATT65280.2666687363995064No Hit
CTGTAGGACGTGGAATATGGCAAGA60060.2453450414852076No Hit
GTCCTAAAGTGTGTATTTCTCATTT46860.19142305434560156No Hit
CTTTAGGACGTGAAATATGGCGAGG46730.19089200447225693No Hit
GGTATCAACGCAGAGTACTTTTTTT43460.17753405765812724No Hit
TATCAACGCAGAGTACTTTTTTTTT42420.1732856586713704No Hit
GTCCTACAGTGTGCATTTCTCATTT28470.11629992226246855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGGT604.1044122E-412.66267711
TCCAACG4400.012.51878418
GCCGGTT2150.012.368196511
TAGGACC9450.012.2649464
TTGGCGA550.003076562212.08710117
GTGCGCC1103.8342478E-812.08710111
CGAACCG658.0475514E-411.68862512
CCAACGT1900.011.49637819
CCAACGA3100.011.02878419
TGTAGGA30850.011.0246672
CCGGTTT2250.010.97431912
CCATTCG700.001495858710.8537249
CTGTAGG30550.010.8056821
GGCGAGG12400.010.79901719
GTCCTAT3650.010.7171241
AGGACCT19650.010.6848235
GGCGAAC803.7833417E-410.68413410
AGGACGT30250.010.6466255
GTAGGAC31350.010.6064023
GGTATCA18000.010.5479441
Produced by FastQC (version 0.11.5)