FastQCFastQC Report
Thu 26 May 2016
SRR1512201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339390
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46670.19949644992925505No Hit
GTATCAACGCAGAGTACTTTTTTTT46360.1981713181641368No Hit
CTGTAGGACGTGGAATATGGCAAGA41310.17658449424850067No Hit
GTCCTAAAGTGTGTATTTCTCATTT37790.16153783678651273No Hit
CTTTAGGACGTGAAATATGGCGAGG34560.1477308187176999No Hit
GGTATCAACGCAGAGTACTTTTTTT29190.12477611685097398No Hit
TATCAACGCAGAGTACTTTTTTTTT29040.12413492406140063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGC500.001595074313.1918748
TCCAACG3250.012.7583418
CCCCTAG1256.330083E-912.2484631
TAGGACC7850.011.7639574
GCCGGTT1850.011.71479111
CCAACGA2450.011.53931619
CGGTTTC2000.011.30780413
GGTCTAA1001.0840815E-511.2277571
GTCCTAC23900.011.0611991
GGACGTG24750.011.0032166
ATACCGT600.00624320510.9936976
CGCATAT650.006249230410.992211
AGGACGT25900.010.9512515
GACGTGA13450.010.9294767
TTAGGAC14800.010.8875813
CGAAATC1659.276846E-1110.85092213
GGTATCA14150.010.6759281
GGCGAGG12100.010.66989219
CCCGTTT2300.010.65227912
TCCTACA25950.010.6200552
Produced by FastQC (version 0.11.5)