FastQCFastQC Report
Thu 26 May 2016
SRR1512201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339390
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60070.256776339131141No Hit
GTCCTACAGTGGACATTTCTAAATT50200.2145858535772146No Hit
CTGTAGGACGTGGAATATGGCAAGA47650.2036855761544676No Hit
GTCCTAAAGTGTGTATTTCTCATTT40390.17265184513911747No Hit
GGTATCAACGCAGAGTACTTTTTTT39670.1695741197491654No Hit
CTTTAGGACGTGAAATATGGCGAGG39460.1686764498437627No Hit
TATCAACGCAGAGTACTTTTTTTTT39450.16863370365779112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG400.005278344314.2507384
TCCAACG2350.012.52800918
TAGGACC7650.012.0463754
AGGGGCG600.005874898811.0839075
GGCGAGG11700.010.71529619
GTAGGAC22850.010.4775673
GGTATCA16300.010.4753871
GTATAGG2750.010.4062251
TGTAGGA24550.010.2938122
CCAACGT1951.4551915E-1110.22823719
TAGGACA6700.010.2094834
GTCCTAC24850.010.1724171
ACCTCGT750.002655282610.1301768
CAAGTCG2250.010.1301756
GATATAC7600.010.0410941
CTGTAGG24950.09.9787151
CCGGTTT2002.5465852E-119.97189112
TTAGGAC17150.09.9713623
AGGACGT26300.09.9700985
GAACAGT6350.09.8709396
Produced by FastQC (version 0.11.5)