##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512201_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2339390 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.907542137052822 31.0 31.0 34.0 28.0 34.0 2 31.1018346663019 31.0 31.0 34.0 27.0 34.0 3 31.191879934512844 31.0 31.0 34.0 27.0 34.0 4 34.806526487674134 37.0 35.0 37.0 32.0 37.0 5 34.42351852405969 37.0 35.0 37.0 30.0 37.0 6 34.35372981845695 37.0 35.0 37.0 30.0 37.0 7 34.17755910728865 37.0 35.0 37.0 30.0 37.0 8 34.07542436276123 37.0 35.0 37.0 30.0 37.0 9 35.57581292559171 39.0 35.0 39.0 30.0 39.0 10 35.37896374695968 39.0 35.0 39.0 30.0 39.0 11 35.37326397052223 39.0 35.0 39.0 30.0 39.0 12 35.19183975309803 38.0 35.0 39.0 29.0 39.0 13 35.17374871227115 38.0 35.0 39.0 29.0 39.0 14 36.18693975780011 39.0 36.0 41.0 29.0 41.0 15 36.11751653208742 39.0 36.0 41.0 28.0 41.0 16 36.016686828617715 39.0 36.0 41.0 28.0 41.0 17 35.94860668806826 39.0 36.0 41.0 27.0 41.0 18 35.87654730506671 39.0 36.0 41.0 27.0 41.0 19 35.902427555901326 39.0 36.0 41.0 27.0 41.0 20 35.77165500408226 39.0 36.0 41.0 27.0 41.0 21 35.65740898268352 39.0 35.0 41.0 27.0 41.0 22 35.74772098709493 39.0 36.0 41.0 27.0 41.0 23 35.64405806641902 39.0 36.0 41.0 27.0 41.0 24 35.518745057472245 39.0 36.0 41.0 27.0 41.0 25 35.07230902072762 39.0 35.0 40.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24987414399385344 1101 2 0.21769652404095652 1101 3 0.34252080211371094 1101 4 0.2749204525611546 1101 5 0.24866711503319294 1101 6 0.22293931300952607 1101 7 0.3120253380304021 1101 8 0.13232215552135784 1101 9 0.1699838022683693 1101 10 0.4176749713086423 1101 11 0.4075879895102261 1101 12 0.3657393859455027 1101 13 0.35491945191115093 1101 14 0.3062625591296353 1101 15 0.31384296743752316 1101 16 0.13867804869089895 1101 17 0.27992410032675963 1101 18 0.47178536091324474 1101 19 0.3823075670596481 1101 20 0.3814872504573046 1101 21 0.37547042139802045 1101 22 0.19732830961228132 1101 23 0.20066087316746462 1101 24 0.21933013345117303 1101 25 0.2995798054718719 1102 1 0.45296865193920866 1102 2 0.4386032170134442 1102 3 0.3749370059266255 1102 4 0.3922629929312933 1102 5 0.42002522444538926 1102 6 0.504061313395539 1102 7 0.5041164362268802 1102 8 0.5526455455408694 1102 9 0.6732734593634362 1102 10 0.5083292570841991 1102 11 0.7224128913995216 1102 12 0.7869806203174861 1102 13 0.8111674414460097 1102 14 0.8455025496938475 1102 15 0.8918983383281329 1102 16 0.9768522246682565 1102 17 0.8481104999321758 1102 18 1.0120525651336152 1102 19 1.0047175518337212 1102 20 1.0161487417399258 1102 21 0.782163393884133 1102 22 0.9559717013473943 1102 23 0.8812832059503748 1102 24 1.1467711061946275 1102 25 1.0933736336119537 1103 1 0.2719088821868034 1103 2 0.16274605624854033 1103 3 0.23680988046311668 1103 4 0.3146427841961241 1103 5 0.2932863375109491 1103 6 0.21616447596392874 1103 7 0.24798034186337503 1103 8 0.3140606745951757 1103 9 0.4268139103963904 1103 10 0.4968540792630165 1103 11 0.2843961586185557 1103 12 0.38257906592381374 1103 13 0.34910815289247665 1103 14 0.36406509948175625 1103 15 0.5434064317696752 1103 16 0.627376488818129 1103 17 0.36330465761174224 1103 18 0.48087734808866145 1103 19 0.5100972743558501 1103 20 0.38015142674876046 1103 21 0.4746898497129095 1103 22 0.7744553992191996 1103 23 0.7202607190013808 1103 24 0.6932108410904405 1103 25 0.9395100919073727 1104 1 0.30775315849999174 1104 2 0.27283680797742704 1104 3 0.3201527016406267 1104 4 0.4063399018489875 1104 5 0.5139915699671249 1104 6 0.4797230047768082 1104 7 0.45559354782390216 1104 8 0.5435656328426646 1104 9 0.6941470032378447 1104 10 0.5964990141724087 1104 11 0.595830748621843 1104 12 0.6532976213106565 1104 13 0.771343784601136 1104 14 0.8520269227937334 1104 15 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-0.6588754635524623 2314 19 -0.5711110147162657 2314 20 -0.7092862450269166 2314 21 -0.6786903583569668 2314 22 -0.5768414122870311 2314 23 -0.6709817987452595 2314 24 -0.6561111613713209 2314 25 -0.7953124416889992 2315 1 -0.1854554379228155 2315 2 -0.10865070458562798 2315 3 -0.22853365720586893 2315 4 -0.20946786307062126 2315 5 -0.15625245783338926 2315 6 -0.3142039213341121 2315 7 -0.4201453240034354 2315 8 -0.20197460692060076 2315 9 -0.236798021792616 2315 10 -0.21023794123080108 2315 11 -0.13115632548632306 2315 12 -0.1821284866338857 2315 13 -0.10560514690985201 2315 14 -0.39103102525906763 2315 15 -0.39103240733741984 2315 16 -0.49598109983573835 2315 17 -0.34134379176867213 2315 18 -0.3806550051464441 2315 19 -0.6152052857836665 2315 20 -0.48374530612486666 2315 21 -0.2679632919878756 2315 22 -0.20185660869778133 2315 23 -0.2747716845627082 2315 24 -0.09861677909209021 2315 25 -0.16338200240227252 2316 1 -0.7996296070953441 2316 2 -0.8187857406392958 2316 3 -0.8965220502726048 2316 4 -0.827130780445593 2316 5 -0.8985339135941004 2316 6 -1.0866112647077983 2316 7 -1.0209527221712165 2316 8 -0.903179654494302 2316 9 -1.354041233329788 2316 10 -1.2836784670126846 2316 11 -1.39403429458077 2316 12 -1.4607187215786368 2316 13 -1.2943916086659257 2316 14 -1.570136041030473 2316 15 -1.693823918839655 2316 16 -1.5575680284278093 2316 17 -1.6409442112934656 2316 18 -1.5267698144563724 2316 19 -1.4549429801955753 2316 20 -1.418891400567425 2316 21 -1.4481993410684169 2316 22 -1.412018951277922 2316 23 -1.417959264407898 2316 24 -1.378021080428212 2316 25 -1.3759771905397145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31826.0 3 3.0 4 528.0 5 5371.0 6 6793.0 7 5520.0 8 4547.0 9 4136.0 10 3906.0 11 3756.0 12 3632.0 13 3525.0 14 3444.0 15 3439.0 16 3659.0 17 3803.0 18 3997.0 19 4583.0 20 5596.0 21 7210.0 22 9484.0 23 12070.0 24 14951.0 25 17982.0 26 20934.0 27 24007.0 28 28212.0 29 34857.0 30 44584.0 31 58026.0 32 74208.0 33 97086.0 34 125719.0 35 166437.0 36 238761.0 37 404474.0 38 858324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.74438112049618 19.67440634513975 13.829638355195264 24.751574179168806 2 14.847032773500784 20.47619251172314 40.9379368125879 23.738837902188177 3 18.87500587760057 24.459624090040567 30.1284950350305 26.536874997328365 4 12.956582698908688 17.154300907501526 37.78826959164569 32.100846801944094 5 13.299185164660365 37.719059689596314 35.736618672796745 13.245136472946578 6 32.08951906266164 37.13476590051253 17.702819965888544 13.072895070937296 7 28.280962131153846 31.58519956056921 21.81949140587931 18.31434690239763 8 25.111503426106808 37.81524243499374 20.065658141652314 17.007595997247147 9 26.139805675838574 15.681481069851541 20.273233620730192 37.905479633579695 10 15.271374161640427 29.353891399039924 33.84976425478436 21.524970184535285 11 34.52763327192132 22.32693137954766 24.535370331582165 18.610065016948862 12 23.44346175712472 24.477064533916963 31.669922501164834 20.409551207793484 13 29.057232868397314 21.85300441568101 25.907522901269136 23.18223981465254 14 20.90450929515814 22.323126968996192 27.28125707983705 29.49110665600862 15 22.873013905334297 31.32782477483447 23.233706222562294 22.565455097268945 16 22.08528719025045 28.2753196346056 27.816653059130797 21.822740116013147 17 21.66299761903744 28.878382826292327 27.859185514172495 21.599434040497737 18 21.52377329132808 27.513881823894266 30.04565292661762 20.916691958160033 19 23.6088040044627 26.83862032410158 27.48575483352498 22.066820837910736 20 23.556696403763375 27.40936739919381 28.103137997512174 20.93079819953065 21 22.827660202018475 26.835756329641487 27.26578296051535 23.07080050782469 22 23.191729467938224 27.945319078905186 27.526406456383928 21.336544996772663 23 22.375961254857035 27.67281214333651 27.905522379765667 22.045704222040786 24 23.17544317108306 26.759112418194487 28.482467651823768 21.58297675889869 25 22.788580701452144 27.322160725331763 28.18868406866695 21.70057450454914 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 317.0 1 317.0 2 420.5 3 524.0 4 524.0 5 524.0 6 1328.5 7 2133.0 8 2133.0 9 2133.0 10 3087.5 11 4042.0 12 4042.0 13 4042.0 14 6683.5 15 9325.0 16 9325.0 17 9325.0 18 17259.5 19 25194.0 20 25194.0 21 25194.0 22 41299.0 23 57404.0 24 57404.0 25 57404.0 26 84025.0 27 110646.0 28 110646.0 29 110646.0 30 145318.5 31 179991.0 32 179991.0 33 179991.0 34 202489.5 35 224988.0 36 224988.0 37 224988.0 38 242723.0 39 260458.0 40 260458.0 41 260458.0 42 278142.5 43 295827.0 44 295827.0 45 295827.0 46 300733.0 47 305639.0 48 305639.0 49 305639.0 50 295033.5 51 284428.0 52 284428.0 53 284428.0 54 253806.0 55 223184.0 56 223184.0 57 223184.0 58 190898.5 59 158613.0 60 158613.0 61 158613.0 62 130019.0 63 101425.0 64 101425.0 65 101425.0 66 78038.5 67 54652.0 68 54652.0 69 54652.0 70 40018.0 71 25384.0 72 25384.0 73 25384.0 74 17745.5 75 10107.0 76 10107.0 77 10107.0 78 6842.5 79 3578.0 80 3578.0 81 3578.0 82 2355.5 83 1133.0 84 1133.0 85 1133.0 86 732.0 87 331.0 88 331.0 89 331.0 90 194.0 91 57.0 92 57.0 93 57.0 94 32.0 95 7.0 96 7.0 97 7.0 98 5.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42412765720978546 2 0.0 3 0.0 4 0.0 5 0.03240160896643997 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0067111511975344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2339390.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.41995108018162 #Duplication Level Percentage of deduplicated Percentage of total 1 83.41053439447876 58.73765751631 2 11.184701681161089 15.75252290467578 3 2.7864871529759205 5.886728669943566 4 1.03784169420039 2.9233904533825714 5 0.5011979150642684 1.76471663301574 6 0.2710535141274102 1.1452545122978126 7 0.1768581899096439 0.8718041557096616 8 0.1182082438401903 0.6659374998800305 9 0.08322172238426048 0.5274422657197271 >10 0.3882096226007683 4.650714024820002 >50 0.022077089327907935 1.0842256122358946 >100 0.015546083125145686 2.1536281156364687 >500 0.002182941566466879 1.061540219074895 >1k 0.0017584807063155124 2.303576435973846 >5k 1.2127453147003532E-4 0.47086098132395876 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6007 0.256776339131141 No Hit GTCCTACAGTGGACATTTCTAAATT 5020 0.2145858535772146 No Hit CTGTAGGACGTGGAATATGGCAAGA 4765 0.2036855761544676 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4039 0.17265184513911747 No Hit GGTATCAACGCAGAGTACTTTTTTT 3967 0.1695741197491654 No Hit CTTTAGGACGTGAAATATGGCGAGG 3946 0.1686764498437627 No Hit TATCAACGCAGAGTACTTTTTTTTT 3945 0.16863370365779112 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.549237194311338E-5 2 0.0 0.0 0.0 0.0 8.549237194311338E-5 3 0.0 0.0 0.0 0.0 8.549237194311338E-5 4 0.0 0.0 0.0 0.0 8.549237194311338E-5 5 0.0 0.0 0.0 0.0 8.549237194311338E-5 6 0.0 0.0 0.0 0.0 8.549237194311338E-5 7 0.0 0.0 0.0 0.0 8.549237194311338E-5 8 0.0 0.0 0.0 0.0 8.549237194311338E-5 9 0.0 0.0 0.0 0.0 8.549237194311338E-5 10 0.0 0.0 0.0 0.0 8.549237194311338E-5 11 0.0 0.0 0.0 0.0 8.549237194311338E-5 12 0.0 0.0 0.0 0.0 8.549237194311338E-5 13 0.0 0.0 0.0 0.0 8.549237194311338E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGAACG 40 0.0052783443 14.250738 4 TCCAACG 235 0.0 12.528009 18 TAGGACC 765 0.0 12.046375 4 AGGGGCG 60 0.0058748988 11.083907 5 GGCGAGG 1170 0.0 10.715296 19 GTAGGAC 2285 0.0 10.477567 3 GGTATCA 1630 0.0 10.475387 1 GTATAGG 275 0.0 10.406225 1 TGTAGGA 2455 0.0 10.293812 2 CCAACGT 195 1.4551915E-11 10.228237 19 TAGGACA 670 0.0 10.209483 4 GTCCTAC 2485 0.0 10.172417 1 ACCTCGT 75 0.0026552826 10.130176 8 CAAGTCG 225 0.0 10.130175 6 GATATAC 760 0.0 10.041094 1 CTGTAGG 2495 0.0 9.978715 1 CCGGTTT 200 2.5465852E-11 9.971891 12 TTAGGAC 1715 0.0 9.971362 3 AGGACGT 2630 0.0 9.970098 5 GAACAGT 635 0.0 9.870939 6 >>END_MODULE