FastQCFastQC Report
Thu 26 May 2016
SRR1512200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082870
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43950.21100692794077405No Hit
GTCCTACAGTGGACATTTCTAAATT27170.13044501097043984No Hit
TATCAACGCAGAGTACTTTTTTTTT26370.12660415676446443No Hit
GGTATCAACGCAGAGTACTTTTTTT25100.12050680071247846No Hit
CTGTAGGACGTGGAATATGGCAAGA23620.11340122043142396No Hit
TTGTAGAACAGTGTATATCAATGAG21870.10499935185585274No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG402.932549E-416.49464413
TAGGACC5250.012.0290364
CCAACGA2650.011.73827419
ATCCCGT2100.011.6696810
GAGGCGT908.144942E-611.5203466
TACACCG1157.89114E-811.4748115
TCCAACG3600.011.2590618
GTCCTAC13550.010.821011
GTATTAA2500.010.58956051
TGTAGAA10200.010.5815742
GCCGGTT2500.010.55657111
TGGCGAG13550.010.43489618
GGCGAGG6400.010.309895519
GTTACAA9750.010.24746815
CCGTTTC2400.010.2109713
GTCTATA2203.6379788E-1210.1822691
GTACTAA1404.1008025E-710.1822691
TGAGTTA11200.010.09876212
TCCTACA15000.010.0465062
CTAGACA2450.010.0028234
Produced by FastQC (version 0.11.5)