FastQCFastQC Report
Thu 26 May 2016
SRR1512200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082870
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57060.27394892624119604No Hit
GGTATCAACGCAGAGTACTTTTTTT36300.1742787595961342No Hit
TATCAACGCAGAGTACTTTTTTTTT35570.17077398013318162No Hit
GTCCTACAGTGGACATTTCTAAATT30960.1486410577712483No Hit
CTGTAGGACGTGGAATATGGCAAGA27110.1301569469049917No Hit
TTGTAGAACAGTGTATATCAATGAG26300.12626808202144157No Hit
GATATACACTGTTCTACAAATCCCG21560.10351102085103728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2450.014.34441919
TCCAACG3550.013.64352418
GTGCGAA500.00150346413.29574911
ACCGTGC957.422386E-812.9958458
TAGGACC5250.012.6671784
CCCTTCG752.0806302E-411.39635613
AAACCGG600.005884483511.08138719
GTAGGAC14250.011.0671153
GTCCTAC13250.011.0157551
GATATAC9750.010.9585261
TGTAGGA15250.010.7774852
TGTAGAA11350.010.7140892
TTAGACC909.513822E-510.5559834
ACTGTTC11000.010.5329958
CTGTAGG16300.010.4176831
AGGACGT12650.010.2138525
CGGTTTC2700.010.20044213
GGACGTG12200.010.1975586
GGTATCA15650.010.1798371
GTAGAAC11200.010.1789843
Produced by FastQC (version 0.11.5)