FastQCFastQC Report
Thu 26 May 2016
SRR1512199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835759
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT65170.22981501601511273No Hit
GTATCAACGCAGAGTACTTTTTTTT56500.19924119080641195No Hit
CTGTAGGACGTGGAATATGGCAAGA56330.19864170403761394No Hit
GTCCTAAAGTGTGTATTTCTCATTT47030.16584625139160275No Hit
CTTTAGGACGTGAAATATGGCGAGG42780.1508590821716514No Hit
GGTATCAACGCAGAGTACTTTTTTT34690.12233056476237932No Hit
TATCAACGCAGAGTACTTTTTTTTT32640.11510145960922631No Hit
GTCCTACAGTGTGCATTTCTCATTT29350.10349962743660517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG904.7293724E-1116.9837511
AATAGCG604.3791157E-412.5658985
TAGGACC10150.012.4420974
TCCAACG3750.012.06517618
CGGTTTC3050.011.74335513
GGCGAGG14000.011.4457619
GCCGGTT3050.011.43230211
TGCCGGT3150.011.06937210
TGTAGGA30900.011.0476842
AAGACGT1201.4255784E-710.9951615
CCGGTTT3100.010.94584412
AGGACGT33450.010.8754195
GGACGTG33050.010.8074326
CTGTAGG31000.010.71621
TTAGGAC19050.010.6859623
GTAGGAC32050.010.6447483
GGTATCA19250.010.640211
GACGTGA17050.010.6128417
GTCCTAC32700.010.5953681
TAGGACG35400.010.5159534
Produced by FastQC (version 0.11.5)