##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512199_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2835759 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.334248079614664 31.0 28.0 33.0 16.0 34.0 2 25.560861131005844 31.0 25.0 33.0 2.0 34.0 3 27.170110365514137 31.0 25.0 34.0 16.0 34.0 4 31.555234066082484 35.0 32.0 37.0 19.0 37.0 5 31.937594132646673 35.0 32.0 37.0 19.0 37.0 6 32.12529308731807 35.0 33.0 37.0 21.0 37.0 7 32.08756914815399 35.0 33.0 37.0 19.0 37.0 8 32.08064824972785 35.0 33.0 37.0 19.0 37.0 9 33.477875588158234 38.0 33.0 39.0 19.0 39.0 10 33.31902887375126 38.0 33.0 39.0 18.0 39.0 11 33.32763503527627 38.0 33.0 39.0 18.0 39.0 12 33.18586099876612 37.0 33.0 39.0 17.0 39.0 13 33.17749004763804 38.0 33.0 39.0 17.0 39.0 14 34.1165568019003 39.0 33.0 41.0 17.0 41.0 15 34.030726870654384 39.0 33.0 41.0 17.0 41.0 16 33.96070505286239 38.0 33.0 41.0 17.0 41.0 17 33.92964352753531 38.0 33.0 41.0 17.0 41.0 18 33.86198968248007 38.0 33.0 41.0 16.0 41.0 19 33.87104263796747 39.0 33.0 41.0 11.0 41.0 20 33.76686241672864 39.0 33.0 41.0 10.0 41.0 21 33.66755355444521 39.0 33.0 41.0 10.0 41.0 22 33.542073215671714 39.0 33.0 41.0 8.0 41.0 23 33.39687258331897 39.0 33.0 40.0 2.0 41.0 24 33.25241319872387 38.0 33.0 40.0 2.0 41.0 25 33.09539774007594 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0065583958077937 1101 2 4.005822284642125 1101 3 2.529321072569317 1101 4 1.5976740967483387 1101 5 1.160555597915181 1101 6 1.0588797642696406 1101 7 0.9708243509828876 1101 8 0.8969747712428884 1101 9 0.8596341543946195 1101 10 0.8095748866469776 1101 11 0.8293938662144242 1101 12 0.8739574445451197 1101 13 0.8853853935388898 1101 14 0.9331219756655429 1101 15 1.1170275250456143 1101 16 1.298383472785524 1101 17 0.9811341665348294 1101 18 1.025115563506553 1101 19 0.9177403846638796 1101 20 0.9910619016781226 1101 21 0.9314109602163825 1101 22 0.934611337409315 1101 23 1.0941770883881503 1101 24 1.0553541426267472 1101 25 1.0668227290009114 1102 1 1.6539880744426938 1102 2 4.325117590570752 1102 3 2.7643756385308826 1102 4 2.0414022633423663 1102 5 1.5334595874489771 1102 6 1.535904464444343 1102 7 1.5255302503497958 1102 8 1.3518621915261946 1102 9 1.5008601800031656 1102 10 1.3811525480746312 1102 11 1.4344330802376604 1102 12 1.5414701189416462 1102 13 1.5088822847958312 1102 14 1.6385754698558515 1102 15 1.742200456170366 1102 16 1.5196593003223455 1102 17 1.6623177091786872 1102 18 1.7586847487415866 1102 19 1.7333748813789427 1102 20 1.6265942822234294 1102 21 1.9081001030969986 1102 22 1.8083226066710267 1102 23 1.6352569722628303 1102 24 1.6858532336248828 1102 25 1.6558654384650282 1103 1 1.4137312733723846 1103 2 4.128767468724888 1103 3 2.729692468472699 1103 4 1.8707702794899141 1103 5 1.4638276978065612 1103 6 1.2213200841475356 1103 7 1.1870170554740724 1103 8 1.2959509389784571 1103 9 1.406873662446337 1103 10 1.3238014566393872 1103 11 1.1526726229907354 1103 12 1.2643441014132364 1103 13 1.277742639639385 1103 14 1.451870800694536 1103 15 1.4944448834334167 1103 16 1.565992488381852 1103 17 1.671987982070192 1103 18 1.5017958736120463 1103 19 1.6384552041056821 1103 20 1.627363441755172 1103 21 1.724615922509443 1103 22 1.644767317524952 1103 23 1.6062171966801984 1103 24 1.7842195836159576 1103 25 1.7647171249180218 1104 1 1.400214221732309 1104 2 4.163215829435135 1104 3 2.77391959536547 1104 4 1.9197086200346796 1104 5 1.5182637633854803 1104 6 1.185761600847286 1104 7 1.19693013601713 1104 8 1.1565279199581795 1104 9 1.3414795872543834 1104 10 1.3359172403309572 1104 11 1.1850033724962827 1104 12 1.3659467623561454 1104 13 1.336604795403204 1104 14 1.5909673284719474 1104 15 1.3457528644293788 1104 16 1.4166440832143365 1104 17 1.5149998912768297 1104 18 1.3562771295586629 1104 19 1.4051742001359457 1104 20 1.425988251214676 1104 21 1.4539240490780756 1104 22 1.5776491486407451 1104 23 1.5276754997949809 1104 24 1.6867489874644122 1104 25 1.6116979842052714 1105 1 0.8584615150236736 1105 2 3.497752340008187 1105 3 2.2311208261928996 1105 4 1.4080985591221058 1105 5 0.959883970525464 1105 6 0.7643322353411364 1105 7 0.47578538803543324 1105 8 0.6013071904660592 1105 9 0.5501880384028368 1105 10 0.6007478607096317 1105 11 0.6203067104894018 1105 12 0.46555233216716374 1105 13 0.6578762580445101 1105 14 0.7200085275880141 1105 15 0.6696270331002196 1105 16 0.5122369382983436 1105 17 0.6125714367680928 1105 18 0.7492022361141721 1105 19 0.7307944052637936 1105 20 0.7982235048527571 1105 21 0.7010327263913254 1105 22 0.6777542327709298 1105 23 0.49854621915093844 1105 24 0.7245025614063678 1105 25 0.8108170683183502 1106 1 0.5348994067408768 1106 2 3.031304348766003 1106 3 1.6970705472096554 1106 4 0.9765471976853881 1106 5 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17 0.6354100248356431 1107 18 0.5683629864726853 1107 19 0.7139879572910601 1107 20 0.7319125835069187 1107 21 0.7050857142984697 1107 22 0.5828517907107127 1107 23 0.8596123024443543 1107 24 0.7794187676915101 1107 25 0.958135683972472 1108 1 0.7758443637454171 1108 2 3.1270145854482507 1108 3 1.9646826272270452 1108 4 1.1037438643192807 1108 5 0.7592494906613432 1108 6 0.6204851007443892 1108 7 0.6814302408447759 1108 8 0.509485012519427 1108 9 0.6072574747252233 1108 10 0.5597996558011502 1108 11 0.6346487076419649 1108 12 0.6209025525937335 1108 13 0.591288202646659 1108 14 0.710982798088942 1108 15 0.7799588607636636 1108 16 0.7631214038921144 1108 17 0.6676089174557518 1108 18 0.5350112796549666 1108 19 0.6137422496831135 1108 20 0.6703493321481275 1108 21 0.4498661697425419 1108 22 0.7110235686205826 1108 23 0.764498278830132 1108 24 0.8834075791454197 1108 25 0.765802583617706 1109 1 0.5898132890998902 1109 2 3.0855071126623628 1109 3 1.6934008173952293 1109 4 1.000395840124387 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-2.288861743091477 2315 23 -2.2141049859401036 2315 24 -2.172102243111919 2315 25 -2.1568484568848767 2316 1 -1.7735782895640426 2316 2 -0.6122505373728124 2316 3 -0.8453203327029435 2316 4 -1.5885994734491362 2316 5 -1.8015991979100896 2316 6 -1.6251955019653437 2316 7 -1.7098478107452344 2316 8 -1.8098032445756687 2316 9 -1.82625842503737 2316 10 -2.005838154594649 2316 11 -2.4309453291413377 2316 12 -1.9787164085317706 2316 13 -1.954189373879398 2316 14 -2.172278910069661 2316 15 -2.3982164177599756 2316 16 -2.097307474890897 2316 17 -2.2606886225313616 2316 18 -2.654768014752886 2316 19 -2.5756150222042535 2316 20 -2.620987950110216 2316 21 -2.464966414629096 2316 22 -2.418808645358947 2316 23 -2.3148297689597506 2316 24 -2.302123911078315 2316 25 -2.2174710861214493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 161075.0 3 6.0 4 1411.0 5 6897.0 6 8102.0 7 7292.0 8 6719.0 9 6445.0 10 6252.0 11 6273.0 12 5936.0 13 5937.0 14 5965.0 15 6207.0 16 6738.0 17 7098.0 18 7898.0 19 10030.0 20 12913.0 21 16771.0 22 20547.0 23 24061.0 24 28010.0 25 31656.0 26 35174.0 27 39622.0 28 45007.0 29 53479.0 30 65402.0 31 81185.0 32 101994.0 33 129302.0 34 168665.0 35 226009.0 36 307355.0 37 388034.0 38 794292.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.977523830480656 18.72976511755759 13.297357074419935 24.995353977541814 2 15.737165494535244 20.362753863839572 40.81003845251962 23.090042189105564 3 19.284466698333674 23.68339481599106 29.236229171801973 27.795909313873285 4 12.309297087658013 16.490823091807165 38.013068106281246 33.18681171425357 5 13.319890724141226 37.59963381937605 35.70920518986275 13.371270266619979 6 32.32410038822167 36.85436415256488 17.784742031299643 13.036793427913803 7 28.677413700863987 31.249204305443733 21.47661160548503 18.59677038820725 8 24.875809263057967 38.02079090642047 20.049059176044224 17.054340654477336 9 26.091427374470115 15.925224957409991 20.228235192059692 37.7551124760602 10 15.12107268451841 29.334064969890523 33.91484568628309 21.630016659307984 11 34.50652098093834 22.431941680569796 24.39643501606133 18.66510232243053 12 23.628918446381455 24.346293789940876 31.636806295609567 20.387981468068105 13 29.207171695479055 21.836058705976072 25.77204198241106 23.18472761613381 14 20.72939408290784 22.5147765089389 27.267803132221413 29.488026275931844 15 22.834486287445444 31.530006604933636 23.127705845242843 22.50780126237808 16 21.95838927073845 28.30445041345192 28.21286999353612 21.524290322273508 17 21.548441244042593 28.896678309656508 27.988630893712607 21.56624955258829 18 21.278630026158186 27.678057840032643 30.314296788400817 20.72901534540835 19 23.52002082063782 26.86380679125195 27.352513429029273 22.26365895908096 20 23.360447767246797 27.552306102175823 28.34528604158534 20.741960088992048 21 22.691956519209143 26.977261209549745 27.17122703841335 23.159555232827763 22 22.853293978040735 28.357392606071507 27.49005979112778 21.29925362475998 23 22.28545977489798 27.909166439796707 27.83348826318232 21.971885522122996 24 23.08553649697334 26.731601273581646 28.70090581166848 21.481956417776537 25 22.676707225294702 27.430290813192144 28.314699781278236 21.578302180234914 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 238.0 1 238.0 2 416.5 3 595.0 4 595.0 5 595.0 6 1485.0 7 2375.0 8 2375.0 9 2375.0 10 3598.0 11 4821.0 12 4821.0 13 4821.0 14 8079.0 15 11337.0 16 11337.0 17 11337.0 18 20752.5 19 30168.0 20 30168.0 21 30168.0 22 49671.5 23 69175.0 24 69175.0 25 69175.0 26 101487.0 27 133799.0 28 133799.0 29 133799.0 30 176448.0 31 219097.0 32 219097.0 33 219097.0 34 247610.0 35 276123.0 36 276123.0 37 276123.0 38 297893.5 39 319664.0 40 319664.0 41 319664.0 42 338709.0 43 357754.0 44 357754.0 45 357754.0 46 363949.5 47 370145.0 48 370145.0 49 370145.0 50 356112.0 51 342079.0 52 342079.0 53 342079.0 54 304950.0 55 267821.0 56 267821.0 57 267821.0 58 229384.5 59 190948.0 60 190948.0 61 190948.0 62 156423.0 63 121898.0 64 121898.0 65 121898.0 66 94681.5 67 67465.0 68 67465.0 69 67465.0 70 49494.5 71 31524.0 72 31524.0 73 31524.0 74 21974.5 75 12425.0 76 12425.0 77 12425.0 78 8411.0 79 4397.0 80 4397.0 81 4397.0 82 2876.5 83 1356.0 84 1356.0 85 1356.0 86 884.0 87 412.0 88 412.0 89 412.0 90 259.5 91 107.0 92 107.0 93 107.0 94 66.0 95 25.0 96 25.0 97 25.0 98 18.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.512768186577209 3 0.0 4 0.0 5 0.0 6 2.821114206108488E-4 7 0.006559090529202235 8 0.0 9 0.0 10 0.003773240250670103 11 0.0023274192200395025 12 1.7631963788178052E-4 13 0.0 14 2.821114206108488E-4 15 0.0 16 0.0 17 1.410557103054244E-4 18 0.01121392896928124 19 0.003914295960975527 20 0.0 21 0.0073348969358820685 22 0.0019042520891232296 23 0.0023626831476158587 24 0.010261802924719626 25 0.006805938022236727 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2835759.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.45527469676779 #Duplication Level Percentage of deduplicated Percentage of total 1 85.77367956595093 63.00529194269463 2 9.894893374204841 14.53664221794888 3 2.2102458505345806 4.870626482952264 4 0.8217605096785232 2.4145057589356727 5 0.37653744934260697 1.3829330887540738 6 0.2233212399560232 0.9842473815955486 7 0.14141331470158316 0.7271287714029684 8 0.10019841624095628 0.5888081751328418 9 0.06988382275749774 0.4620001857761007 >10 0.351879925467848 4.3979141600614575 >50 0.018753491860369446 0.9632177655268455 >100 0.014027008114590164 2.0795533574905813 >500 0.001751673183456242 0.9243483602248458 >1k 0.001508385240991839 2.026582490336798 >5k 1.4597276525727472E-4 0.6361998611663817 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6517 0.22981501601511273 No Hit GTATCAACGCAGAGTACTTTTTTTT 5650 0.19924119080641195 No Hit CTGTAGGACGTGGAATATGGCAAGA 5633 0.19864170403761394 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4703 0.16584625139160275 No Hit CTTTAGGACGTGAAATATGGCGAGG 4278 0.1508590821716514 No Hit GGTATCAACGCAGAGTACTTTTTTT 3469 0.12233056476237932 No Hit TATCAACGCAGAGTACTTTTTTTTT 3264 0.11510145960922631 No Hit GTCCTACAGTGTGCATTTCTCATTT 2935 0.10349962743660517 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.52639275763561E-5 2 0.0 0.0 0.0 0.0 3.52639275763561E-5 3 0.0 0.0 0.0 3.52639275763561E-5 3.52639275763561E-5 4 0.0 0.0 0.0 3.52639275763561E-5 3.52639275763561E-5 5 0.0 0.0 0.0 3.52639275763561E-5 3.52639275763561E-5 6 3.52639275763561E-5 0.0 0.0 3.52639275763561E-5 7.05278551527122E-5 7 3.52639275763561E-5 3.52639275763561E-5 0.0 3.52639275763561E-5 7.05278551527122E-5 8 3.52639275763561E-5 3.52639275763561E-5 0.0 3.52639275763561E-5 7.05278551527122E-5 9 3.52639275763561E-5 3.52639275763561E-5 0.0 3.52639275763561E-5 7.05278551527122E-5 10 7.05278551527122E-5 3.52639275763561E-5 0.0 3.52639275763561E-5 7.05278551527122E-5 11 7.05278551527122E-5 3.52639275763561E-5 0.0 3.52639275763561E-5 7.05278551527122E-5 12 1.410557103054244E-4 3.52639275763561E-5 0.0 7.05278551527122E-5 1.410557103054244E-4 13 1.763196378817805E-4 3.52639275763561E-5 0.0 7.05278551527122E-5 1.410557103054244E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 90 4.7293724E-11 16.983751 1 AATAGCG 60 4.3791157E-4 12.565898 5 TAGGACC 1015 0.0 12.442097 4 TCCAACG 375 0.0 12.065176 18 CGGTTTC 305 0.0 11.743355 13 GGCGAGG 1400 0.0 11.44576 19 GCCGGTT 305 0.0 11.432302 11 TGCCGGT 315 0.0 11.069372 10 TGTAGGA 3090 0.0 11.047684 2 AAGACGT 120 1.4255784E-7 10.995161 5 CCGGTTT 310 0.0 10.945844 12 AGGACGT 3345 0.0 10.875419 5 GGACGTG 3305 0.0 10.807432 6 CTGTAGG 3100 0.0 10.7162 1 TTAGGAC 1905 0.0 10.685962 3 GTAGGAC 3205 0.0 10.644748 3 GGTATCA 1925 0.0 10.64021 1 GACGTGA 1705 0.0 10.612841 7 GTCCTAC 3270 0.0 10.595368 1 TAGGACG 3540 0.0 10.515953 4 >>END_MODULE