FastQCFastQC Report
Thu 26 May 2016
SRR1512199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835759
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT74340.2621520376026312No Hit
GTATCAACGCAGAGTACTTTTTTTT68450.2413815842601575No Hit
CTGTAGGACGTGGAATATGGCAAGA66110.2331298252072902No Hit
GTCCTAAAGTGTGTATTTCTCATTT54770.19314053133570236No Hit
CTTTAGGACGTGAAATATGGCGAGG52260.18428928551403698No Hit
TATCAACGCAGAGTACTTTTTTTTT42920.1513527771577204No Hit
GGTATCAACGCAGAGTACTTTTTTT42120.14853166295161188No Hit
GTCCTACAGTGTGCATTTCTCATTT32230.11365563857859572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCT456.781538E-414.77295114
ACCGTGC1257.2759576E-1213.6755318
TAGGACC12500.012.2371814
CATACCG701.0905292E-412.2154655
TCCAACG3900.011.44497818
CGGTTTC1850.011.29360813
TGTAGGA37800.011.1346742
GGCGTAG600.005699872511.1291631
GCACCGT1201.2823875E-711.0797136
GTCCCGT600.00589199311.0797136
CTGTAGG37450.010.9784541
AGGACGT33950.010.9421465
CCAACGA2700.010.904613519
ATACCGC700.001496131410.8535966
GGACGTG33750.010.8334976
GTAGGAC37200.010.8034623
GACGTGA15200.010.6840097
GGTATCA18900.010.548731
TAGGACG36150.010.5127724
AGGACCT23500.010.3499365
Produced by FastQC (version 0.11.5)