FastQCFastQC Report
Thu 26 May 2016
SRR1512198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2682817
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49910.1860357974472355No Hit
GTCCTACAGTGGACATTTCTAAATT41450.15450177928647388No Hit
CTGTAGGACGTGGAATATGGCAAGA38500.14350587460866693No Hit
GTCCTAAAGTGTGTATTTCTCATTT33930.12647154092135243No Hit
CTTTAGGACGTGAAATATGGCGAGG31470.1173020746476558No Hit
TATCAACGCAGAGTACTTTTTTTTT29590.11029451505637546No Hit
GGTATCAACGCAGAGTACTTTTTTT29400.10958630424661839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT955.495167E-913.88781456
TAGGACC6850.012.5193084
CGGTAGG708.2326354E-411.6569911
TGTAGGA22150.011.1438732
CTATGCG600.00624099910.9943169
GCGAACC600.00624099910.99431611
TTCCGCC700.00159579710.76994111
GGCGAGG8900.010.69510719
GTAGGAC23250.010.579063
GTATCAA28650.010.4668791
CTGTAGG22450.010.2225851
GGTATCA14500.010.1998671
GTCCTAA11350.010.1549331
GGACGTG21250.010.1112626
ACACCGT1401.09066605E-710.0970096
AGGACGT21850.010.0923885
GTCCTAC19950.010.0720481
TAAGACG750.002831373410.0521334
GACGTGG12000.09.97367
TAGGACG23550.09.9640974
Produced by FastQC (version 0.11.5)